Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

Abstract

Purpose:To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. Methods: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. Results: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). Conclusion: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.

Remote Ischemic Preconditioning and N-Acetylcysteine in Autologous Ovarian Transplantation in Rats.

“…As our study indicated, the number of follicles in the autografted groups compared with the control groups decreased considerably, which confirms previous reports [23,24]. This could be explained because in the first days of transplantation, granulosa cells and oocytes, particularly in developing follicles, undergo apoptosis as a result of ischemia and reperfusion injury induced by free radicals and lipid peroxidation.…”

“…R-IPC leads to earlier re-initiation of ovarian activity and increases the number of cycles in fresh graft, which can be related to a compensatory effect after I/R injury during the transplant when growth factors are produced to establish graft angiogenesis. The established neovascularization may stimulate the graft to re-initiate its function [23] which justifies the findings.…”

Study on the Estrous Cycle Regularity of Cryopreserved Rat Ovarian Tissues after Heterotopic Transplantation

“…The subsequent transplantation is considered as an alternative treatment to restore fertility in cancer female patients who are at risk of ovarian failure after deleterious effects of cancer therapy [2] [4] [5].…”

“…While earlier studies have considered that the major loss of viable follicles quantity occurred after transplantation rather than freeze-thaw procedure because transplantation directly related to time duration for re-establishing of blood supply to graft tissues [5] [8].…”

Autologous and allogeneic ovarian orthotopic transplantation: morphologic, endocrinologic and natural pregnancy assessment

“…The recovery of reproductive capacity has been long neglected by researchers [7][8][9][10] . Over the last few years, scientific advances have permitted the occurrence of pregnancy in cases of ovariectomy by means of various methods, such as ovarian cryopreservation, ovarian follicle transplantation, ovarian implants and others 11 .…”