Study on water purification effects and microbial community compositions of the estuarine wetland buffer zones during a low-temperature period

Sun, Jiajun; Zhang, Panyue; Liang, Jinsong; Cai, Yajing; Han, Yangyang; Wang, Zhi; Li, Yuping

doi:10.1051/e3sconf/202130002009

Cited by 2 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1% agarose gels and Nano Drop 2000 (Thermo Fisher Scienti c, MA, USA) were performed for the DNA concentration and purity control. Broadspectrum primer sets 338F (5 ' -ACT CCT ACG GGA GGC AGC A-3 ') -806R (5 ' -GGA CTA CHV GGG TWT CTA AT-3 '), and ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') -ITS2 (5'-GCTGCGTTCTTCATCGATGC-3') ampli cation of the 16S rRNA V3 + V4 region and the RNA manipulator ITS region of each sample in combination with adapter sequences and barcode sequences were used to evaluate soil bacterial and fungi community [44,45]. The capacity of each PCR reaction system was 20 µL containing 4 µL 5 × FastPfu Buffer, 2 µL 2.5 mM dNTPs, 0.8 µL 5 µM primers, 0.4 µL FastPfu Polymerase and 10 ng DNA template.…”

Section: Determination Of Ginsenoside Content Treated With Pp-7250 Co...mentioning

confidence: 99%

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Li,

Liu,

Gao

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: The ginseng endophyte Paenibacillus polymyxa Pp-7250 (Pp-7250) have multifaceted roles such as preventing ginseng diseases, promoting growth, increasing ginsenoside accumulation and degrading pesticide residues, these effects still room for improvements. Composite fungicide is an effective means to improve the biocontrol effect of fungicide, but the effect of Pp-7250 in combination with its symbiotic bacteria on ginseng needs to be further researched, and its mechanism of action still remains to be elucidated. In order to elucidate the effect of Paenibacillus polymyxa and Bacillus cereus co-bacterial agent on the yield and quality of understory ginseng, as well as to investigate its mechanism of action. Firstly, the effects of Bacillus cereus (BS), Paenibacillus polymyxa Pp-7250(PS) and Paenibacillus polymyxa and Bacillus cereus co-bacterial agent (PB) treatments on ginseng yield, ginsenoside content, disease prevention and pesticide degradation rate were evaluated by field experiments. Secondly, differences in microbial communities in ginseng rhizosphere soils with different treatments were analyzed using high‐throughput amplicon sequencing. Finally, correlation analysis was performed to elucidate the mechanism of action of PB co-bacterial agent in improving the yield and quality of ginseng. Results: The results indicate that PB treatment improved ginseng yield, ginsenoside accumulation, disease prevention and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, Trechisporaincreased and decreased the abundance of pathogenic microorganisms Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agent enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogen and animal pathogen. This study provided a theoretical reference for indepth research on improving the yield and quality of ginseng. Conclusion: The combined agent of Paenibacillus polymyxa and Bacillus cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease, improve the yield, quality and pesticide degradation.

show abstract

Section: Determination Of Ginsenoside Content Treated With Pp-7250 Co...mentioning

confidence: 99%

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Li,

Liu,

Gao

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Effects of a co-bacterial agent on the growth, disease control, and quality of ginseng based on rhizosphere microbial diversity

Li,

Liu,

Gao

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background The ginseng endophyte Paenibacillus polymyxa Pp-7250 (Pp-7250) has multifaceted roles such as preventing ginseng diseases, promoting growth, increasing ginsenoside accumulation, and degrading pesticide residues, however, these effects still have room for improvements. Composite fungicides are an effective means to improve the biocontrol effect of fungicides, but the effect of Pp-7250 in combination with its symbiotic bacteria on ginseng needs to be further investigated, and its mechanism of action has not been elucidated. In this study, a series of experiments was conducted to elucidate the effect of Paenibacillus polymyxa and Bacillus cereus co-bacterial agent on the yield and quality of understory ginseng, and to investigate their mechanism of action. Results The results indicated that P. polymyxa and B. cereus co-bacterial agent (PB) treatment improved ginseng yield, ginsenoside accumulation, disease prevention, and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms, including Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, and Trechispora, and decreased the abundance of pathogenic microorganisms, including Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agents enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, and terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogens and animal pathogens. Conclusion The combination of P. polymyxa and B. cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease and improve the yield, quality, and pesticide degradation.

show abstract

Study on water purification effects and microbial community compositions of the estuarine wetland buffer zones during a low-temperature period

Cited by 2 publications

References 18 publications

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

Effects of a co-bacterial agent on the growth, disease control, and quality of ginseng based on rhizosphere microbial diversity

Contact Info

Product

Resources

About