Study Some Kinetic Parameters for Purified Arginase from Two Different Sources: Red Blood Cells and Serum of( Healthy and TypeII Diabetic Patient)

Abstract

Kinetics parameters for arginase that purified from RBCs, and serum (control, and diabetic patients) revealed that the Km values are (200, 416, and 333mM), Vmax values are (47, 5.5, and 9 U/min),and The values of catalytic constant Kcat are (174, 45.8, and 81.8 min -1 ). The effect of atrovastatin was also studied on serum arginase, the arginase activity is decreasing by increasing statin concentration, wherein at the lower concentration (0.112 mg/ml) the allosteric regulation percentage is 26% and it is ongoing until reaching its maximum (85%) at the highest concentration (0.9mg/ml). In conclusion affinity of arginase to the L-arginine, and its velocity is more in RBCs, and serum patients compared with serum controls, and statin appears to be an allosteric regulator for arginase.