The selection of tools, especially the use of vacutainer tubes, affects the results of the examination to determine the stability of serum from plain vacutainer with vacutainer separation gel on the quality of uric acid and glucose test results. This study used an experimental design. The study took blood samples of 3 ml each in SST vacutainer and plain vacutainer serum. The mean uric acid levels using plain vacutainer and SST vacutainer were 4.5875±0.59048 and 4.9250±0.56318, respectively. The mean glucose levels using plain vacutainer and SST vacutainer were 90.64±12.33 and 94.12±13.30, respectively. Uric acid and glucose levels using gel-separated vacutainer were higher than uric acid levels using plain vacutainer. There was a significant difference in uric acid levels between regular vacutainers and SST vacutainers, similar to glucose levels (p = 0.000, p = 0.000). Uric acid and glucose levels using SST vacutainer were higher than uric acid levels using plain vacutainer. The following types of vacutainer separator gel and plain vacutainer affect the quality of uric acid and glucose test results.