Study the influence of Mutagenic Treatment on Cell Suspension Culture of Elephantopus scaber L. for the Quantitative enhancement of Bioactive constituents and their Antioxidant potentials

Abstract

In the present study, the impact of the chemical mutagen, Ethyl methanesulfonate (EMS) was evaluated on the quantitative enhancement of sesquiterpene, flavonoids (Quercetin and Rutin) and Stigmasterol compound Elephantopus scaber. The leaves of E. scaber L. were used for the callus induction. MS basal medium supplemented with different hormone combinations of 2,4-D and BAP (1:1 ratio) was used. The cell suspension culture was treated with three concentrations of EMS (0.1%, 0.2% and 0.5%) and treatment durations (1h, 2h and 3h). Mutagenesis is an important strategy to improve production of secondary metabolites using cell suspension culture. The results obtained revealed that the concentrations and treatment durations of EMS were most effective in enhancement in the content of studied metabolites. EMS treatment of 0.1% concentration was effective. The analysis was done using HPTLC technique. The highest content of Deoxyelephantopin recorded in 1h EMS treatment and 0.1% concentration compared to control sample. Quercetin, Rutin content was also high in 1h EMS treatment and 0.1% concentration compared to control sample. The Stigmasterol content was more in 2h EMS treatment and 0.1% concentration compared to control sample. The EMS treated samples showed potential scavenging activity compared to control samples IC50 value. The method was simple, precise, eco-friendly.