Study the prevalence of mecA gene in methicillin resistance staphylococcus aureus (MRSA) isolated from different clinical specimens and their antibiotic resistance profile

Abstract

Methicillin-resistant S aureus has progressed into an important pathogen of humans and is endemic in hospitals worldwide. MRSA strains carry multiple antibiotic-resistant genes. Recent work aim to investigate the prevalence of mecA gene among clinical isolates of MRSA .Fifty isolates of Staphylococci were demonstrated as methicillin resistancestaphylococcus aureus from various clinical specimens ( urine ,blood ,wounds ,and burns )after confirming their identity using morphological and biochemical tests ,as well as diagnosis by VITEK2 system. Also antibiotic sensitivity testing performed for all isolates by disc diffusion methods.Fifty isolates of S. aureus classified as MRSA taken from different clinical cases.mecA gene was detected by PCR technique to amplify 533bp using specific primers for the gene. The results showed that MecA gene was detecting in 39(78%) of Staphylococcus aureus isolates.Most of MRSA isolates were multiresistant to three antibiotic classes beta-lactams and multidrug resistant to other common antibiotics, such as aminoglycosides, therefore this work emphasis that there are other possible resistance mechanism may interact with mec A gene and causes development of methicillin resistance staphylococcus aureus