Studying plant genome variation using molecular markers

Gostimsky, S. A.; Кокаева, З.Г.; Коновалов, Ф. А.

doi:10.1007/s11177-005-0101-1

Cited by 62 publications

(37 citation statements)

References 41 publications

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“…At the molecular level, variations in tissue culturederived plants arise from changes in chromosome number or structure, or from more subtle changes in the DNA (Gostimsky et al 2005). Visible morphological variation is known to occur at a much lower frequency than at the DNA level (Evans et al 1984).…”

Section: Molecular Detectionmentioning

confidence: 99%

“…Usage of random primers eradicated the limitation of prior sequence for PCR analysis (Agarwal et al 2008). PCRbased analytical techniques using various molecular markers provide an essential tool needed to reveal polymorphism at the DNA sequence level and solve the problems of introgression as well as lineage (Gostimsky et al 2005;Simmons et al 2007). …”

Section: Polymerase Chain Reaction Based Techniquesmentioning

confidence: 99%

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Somaclonal variation in plants: causes and detection methods

2010

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Plant tissue culture has become one of the fundamental tools of plant science research. It is extensively employed in the production, conservation and improvement of plant resources. The presence of somaclonal variation in populations derived from tissue culture is affecting the use of tissue culture negatively and has remained a major problem. Conversely, it is a source of new desirable clones/variants with better agronomic traits. In this review, we summarize the possible causes, detection methods and desirability of variants. Somaclonal variation is one of the most researched and reviewed topics. Hence, we restricted ourselves to outlining various examples which may be used as important references for researchers who intend to identify and/or characterize somaclonal variants while using tissue culture for research and production. Emphasis is placed on the negative effects of somaclonal variation. However, this review also includes examples of some useful variants generated as a result of somaclonal variation.

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Section: Molecular Detectionmentioning

confidence: 99%

Section: Polymerase Chain Reaction Based Techniquesmentioning

confidence: 99%

Somaclonal variation in plants: causes and detection methods

2010

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“…At the molecular level, variations in tissue culture-derived plants arise from changes in chromosome number or structure, or from more slight changes in the DNA (Gostimsky et al, 2005). Visible morphological variation is known to occur at a much lower frequency than at the DNA level (Evans et al, 1984;Evans, 1989).…”

Section: Introductionmentioning

confidence: 99%

Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods

Mohamed

El-Hamed

Omar

et al. 2017

HJSC

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Ex vitro experiment was conducted at the greenhouse and nursery facilities of the Plant Tissue Culture Laboratory of Department of Horticulture, Faculty of Agriculture, Suez Canal University during the period 2009-2012. Strawberry ex vitro growth performance of tissue culture-derived plants from different propagation methods (meristem, direct regeneration and callus-derived plants) were examined with standard runner-propagated mother plants based on morphological traits and genetic analysis using RAPD. Ex vitro growth performance examination showed that leaf morphology and serration patterns were almost similar among plants obtained from meristem, direct or callus regeneration. Tissue culture plants have higher number of runners while standard runner plants were higher in flower production compared with in vitro propagated plants. RAPD analysis indicated that plants of cv. 'Tudla' from the different in vitro propagation methods were genetically stable, while in cv. 'Festival', callus-derived plants deviated from normal genotype. The obtained results confirmed the usefulness of RAPD in determining genetic stability of plants clonally propagated in vitro as well as detecting the somaclonal variants, which might be used further in breeding programs.

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“…This technique requires neither genomic DNA sequence information nor large amounts of DNA, and allows rapid and easy identifi cation [17]. However, the ISSR technique is quite limited due to low reproducibility, because they are sensitive to various experimental conditions.…”

Section: Development Of An Issr-derived Scar Marker In Korean Ginsengmentioning

confidence: 99%

Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)

Lee¹,

Kim²,

Jo³

et al. 2011

Journal of Ginseng Research

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Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identifi cation of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-fi ve inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplifi ed ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplifi ed region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplifi ed polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specifi c polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identifi cation of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.

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Studying plant genome variation using molecular markers

Cited by 62 publications

References 41 publications

Somaclonal variation in plants: causes and detection methods

Somaclonal variation in plants: causes and detection methods

Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods

Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)

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