Studying the Content of Phenolic Compounds of Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region With HPLC

Mirovich, V.M.; Petukhova, Svetlana Andreyevna; Дударева, Л. В.

doi:10.12737/article_59f035fe65fc57.52277900

Acta Biomedica Scientifica

2017

DOI: 10.12737/article_59f035fe65fc57.52277900

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Studying the Content of Phenolic Compounds of Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region With HPLC

V.M. Mirovich¹,

Svetlana Andreyevna Petukhova²,

Л. В. Дударева³

Abstract: Kromasil C18 column (4,6 × 250 mm, 5 micron). Detection was carried out using a UV detector "GILSTON" UV/VIS model 151, at a wavelength of 254 nm. In comparison with standard samples we identified 8 flavonoids (prevailing -izoquercetin, hyperoside, izoramnetin-3-glucoside), 5 aromatic acids (predominantly acid -cinnamic, gallic, chicory acids -coffee, chlorogenic, gallic, chicory, cinnamon; flavonoids -vitsenin, lyuteolin-7-glucoside, isoramnetin-3-glucoside, apigenin; epicatechin, catechin, epigallocatechi… Show more

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Metabolomic Profile Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region

Petukhova¹,

Оленников²,

Mirovich³

2019

JCPRM

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In folk medicine in the Baikal region, an infusion from the aerial part of the plant Bupleurum scorzonerifolium (Willd) is widely used as a choleretic and anti-inflammatory agent. The choleretic effect of herbal remedies is mainly due to the content of phenylpropanoids and flavonoids. Using high performance liquid chromatography with a diode-matrix detector and 3Q electrospray ionization detector, we studied the metabolic profile of the phenylpropanoids and flavonoids of the aboveground organs of B. scorzonerifolium (Willd.), which grows in the Baikal region. In the aboveground organs of B. scorzonerifolium, 7 phenylpropanoids and 17 flavonoids were identified, of which 15 compounds for the species were identified for the first time. Quantitative determination of individual components was carried out in the aboveground organs of B. scorzonerifolium and dry extract by microcolumn HPLC with UV detection. The predominant components in the aerial part of the plant are 5-O-caffeic quinic acid, 3,5-di-O-caffeic quinic acid, isoquercitrin, isorhamnetin-3-O-glucoside, kaempferol-3-O-rhamnoside and rutin. The dry extract obtained from the aerial part of B. scorzonerifolium contains the entire complex of phenylpropanoids and flavonoids, which are identified in the plant. The content of active ingredients in the extract is 2.5–3.0 times higher than in the aboveground organs. The aboveground organs and dry extract of B. scorzonerifolium can be used as a source of phenylpropanoids and flavonoids.

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Metabolomic Profile Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region

Petukhova¹,

Оленников²,

Mirovich³

2019

JCPRM

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Dynamic Changes in the Composition of Biologically Active Compounds of Bupleurum Scorzonerifolium Willd. Aerial Part in Different Phenological Phases

Tykheev¹,

Taraskin²,

Жигжитжапова³

et al. 2020

JCPRM

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The article is devoted to investigate the changes in the composition of biologically active substances (essential oils, lipid fraction, phenolic compounds) of the aerial part of the Bupleurum scorzonerifolium Willd. in depending on the phase of plant development. The greatest variety of compounds included in the composition of essential oils is characterized by a flowering phase. Fifteen terpene compounds – p-cymol, β-myrcene, trans-β-ocymene, limonene, (E,E)-α-farnesene, α-copaene, β-elemene, humulene, germacrene D, caryophyllene, γ-muurolene, bicyclogermacrene, Δ-cadinene, t-muurolol, spatulenol – are constant components of essential oils, regardless of phenophase. The quantitative content of these components varies from trace to major at different phenological phases. The coincidence of the main components of the lipid fraction was established. The main saturated acid is 16:0. Its content changes depending on the phase of vegetation – it is approximately at the same level determined in the phases of vegetation and flowering phases (26.78% and 27.26%, respectively) and there is a decrease in the fruit phase (18.17%). The main monounsaturated fatty acid is 18:1n9. Its greatest content was found in the fruiting phase, the smallest – flowering. The predominant polyunsaturated fatty acid is 18:2n9,12. Its greatest content was found in the vegetation phase (26.78%), the lowest - flowering (24.23%). However, the total content of unsaturated fatty acids exceeds the total content of saturated fatty acids in all phases of plant development. Although, their ratio varies from phenophase. There is a general pattern of highest content of phenolic compounds in the flowering phase for all studied samples. It was shown that during the flowering phase, the maximum accumulation of essential oils, lipids and phenolic compounds is observed.

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Studying the Content of Phenolic Compounds of Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region With HPLC

Cited by 2 publications

References 2 publications

Metabolomic Profile Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region

Metabolomic Profile Bupleurum Scorzonerifolium Willd., Growing in the Baikal Region

Dynamic Changes in the Composition of Biologically Active Compounds of Bupleurum Scorzonerifolium Willd. Aerial Part in Different Phenological Phases

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