Studying the correlations among hematological and serum biochemical constituents in cattle theileriosis

Ellah, Mahmoud R. Abd

doi:10.1007/s12639-013-0299-0

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…Leukopenia in the terminal stage could have resulted from large-scale destruction of lymphocytes by schizogony in lymphoid organs and infiltration of these cells into various organs (Sandhu et al 1998). The thrombocytopenia with decreased RBCs and WBCs could also be related to increased TNF-α production (Forsyth et al 1999) or suppression of the bone marrow by the parasite and its products (Abd Ellah 2015; Mbassa et al 1994). In this study, the levels of IL-6, TNF-α and IFN-γ were increased from the onset to the middle course of disease and decreased in the terminal stage of disease in infected sheep.…”

Section: Discussionmentioning

confidence: 99%

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

Razmi

Yaghfoori

Mohri

et al. 2019

Onderstepoort Journal of Veterinary Research

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Malignant ovine theileriosis is caused by Theileria lestoquardi , which is highly pathogenic in sheep. Theileriosis involves different organs in ruminants. Little is known about the role of proinflammatory cytokines in the pathogenesis of T. lestoquardi infection. The aim of this study was to measure concentration changes of proinflammatory cytokines and immunoglobulin G (IgG) during an ovine experimental theileriosis and correlate it with clinical and haematological parameters. During an experimental study, seven healthy Baluchi sheep (four females and three males) about 6–8 months old were infected with T. lestoquardi by feeding of infected unfed ticks on the sheep’s ears. The infected sheep were clinically examined during the study and blood samples were collected on days 0, 2, 5, 7, 10, 12, 14, 17 and 21. The haematological parameters were analysed by an automatic veterinary haematology cell counter and the inflammatory cytokines interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and IgG were measured by enzyme-linked immunosorbent assay. All infected sheep had temperatures above 40 °C on days 3–4 post infection (PI). The maximum temperature was noted on day 7, and it remained high until day 21. The parasitaemia of T. lestoquardi infection increased from 0.01% (day 7 PI) to 3.3% (day 21 PI). The mean white blood cell (WBC), red blood cell (RBC), lymphocyte, neutrophil and platelet values slightly increased on day 2 PI and decreased by day 17 and day 21 PI. The percentage parasitaemia and fever had a negative correlation with the numbers of WBCs, RBCs, lymphocytes, neutrophils and platelets. The serum concentration of IL-6, TNF-α and IFN-γ cytokines increased and peaked on day 12 and thereafter decreased to levels lower than 0. Out of all tested cytokines, the concentration of IL-6 was significantly higher, as early as day 2 PI. No significant changes were observed for the IgG levels during the course of disease. A significant and strong correlation was observed between IL-6, TNF-α and IFN-γ values and a moderate correlation between IL-6 and the numbers of lymphocytes in the present study. A strong correlation was determined between the percentage parasitaemia and haematological parameters in T. lestoquardi -infected sheep. In addition, preliminary results indicate that the measurement of the serum concentrations of IL-6 in combination with haematological parameters could be considered a good marker to estimate the pathogenicity of T. lestoquardi strain.

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Section: Discussionmentioning

confidence: 99%

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

Razmi

Yaghfoori

Mohri

et al. 2019

Onderstepoort Journal of Veterinary Research

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“…Notably, MPV (fL) values in whole blood (3.11 ± 0.06) from the cows used in this study were lower than the same parameter reported in healthy cows in which whole blood (6.30 ± 0.17) was obtained with EDTA [39]. However, PDW (%) values in whole blood (16.47 ± 0.10) from our cows were higher than the same value reported for whole blood (10.70 ± 0.30) of healthy cows using EDTA [39]. …”

Section: Discussionmentioning

confidence: 63%

“…However, the values for these parameters could change as a function of the anticoagulant used for blood collection and the technology of the device used for measurement [15, 38, 40, 41]. Notably, MPV (fL) values in whole blood (3.11 ± 0.06) from the cows used in this study were lower than the same parameter reported in healthy cows in which whole blood (6.30 ± 0.17) was obtained with EDTA [39]. However, PDW (%) values in whole blood (16.47 ± 0.10) from our cows were higher than the same value reported for whole blood (10.70 ± 0.30) of healthy cows using EDTA [39].…”

Section: Discussionmentioning

confidence: 94%

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Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Gutiérrez

López

Giraldo

et al. 2017

Veterinary Medicine International

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There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.

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“…Anaplasma marginale and Anaplasma centrale of the order Rickettsiales are the main causes of anaplasmosis, which was once referred to as gall sickness [Di Domenico et al 2016;Sivajothi and Reddy, 2022]. Sheep, goats, and other ruminants in the wild are susceptible to Anaplasma ovis [Abd Ellah, 2015;Hornok et al 2011]. Some forms of Babesia have been found in the family Cervidae [France, 2004].…”

Section: Introductionmentioning

confidence: 99%

Hemato-Chemical Variations- A Diagnostic Tool for Babesia, Theileria and Anaplasma Infections in Captive Hog Deer (Axis Porcinus)

Azhar,

Saleem,

Safdar

et al. 2024

Preprint

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Hog deer are an essential component of Pakistan’s biodiversity. Their conservation helps maintain integrity of local ecosystems, contributing to overall stability and balance of region’s wildlife populations. In Pakistan, they are classified as an endangered species according to IUCN. Theileria, babesia and anaplasma are among most prevalent blood parasites in ruminants which require diagnosis at early stages of the infection so that treatment protocols can be followed and more severe pathological alterations can be prevented. The changes in hemato-chemical parameters caused by Theileria, babesia and anaplasma were recorded individually and then compared with healthy group of hog deer respectively. Alterations in hemato-chemical parameters produced by these parasite-infected hog deer were assessed as potential diagnostic tools for distinguishing between individual blood parasites. Total leukocyte count, monocytes and eosinophils were observed to be higher than normal in babesia infected hog deer while level was lower than normal hog deer in anaplasma and theileria infected hog deer. The main objective of this study is to promote early diagnosis of parasitic infections in hog deer to facilitate prompt treatment, pathological prevention and allocate resources for further research on hemato-chemical parameters as diagnostic tools, enhancing understanding and management of blood parasite infections in hog deer.

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Studying the correlations among hematological and serum biochemical constituents in cattle theileriosis

Cited by 6 publications

References 20 publications

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Hemato-Chemical Variations- A Diagnostic Tool for Babesia, Theileria and Anaplasma Infections in Captive Hog Deer (Axis Porcinus)

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