Studying the expression rate and methylation of Reprimo gene in the blood of patients suffering from gastric cancer

Abstract

As gastric cancer has no exclusive signals in its initial phases, it is usually diagnosed in advanced phases. Although many researches have been conducted on methylation and diagnosis of cancer’s markers, the methylation and expression of Reprimo gene and its correlation with gastric cancer has not been thoroughly studied. Methylation of Reprimo promoter is a repetitive procedure exclusive to cancer which nullifies its expression and performance. The present research seeks to study the expression and methylation of Reprimo among people suffering with gastric cancer so that it may be used as a biomarker for early diagnosis. Fifty blood samples taken from healthy people (normal samples) and 50 blood samples obtained from gastric cancer patients were analyzed using Real-Time PCR. The methylation status of the promoter of Reprimo was studied using Methylation Specific PCR technique in normal samples and in gastric cancer Iranian patients. We observed reduction in expression rate of Reprimo in the blood samples of patients suffering with gastric cancer in comparison to normal blood samples. A significant correlation was also observed between the expression rate of this gene, age and methylation of its promoter among patients suffering with gastric cancer and various analysis points to a correlation between reduced expressions of Reprimo gene in gastric cancer patients. In conclusion, reduced expression of Reprimo gene and greater levels of methylation of its promoter seems to be promising biomarkers for early diagnosis of gastric cancer.

Perfil de citocinas Th1, Th2, Th17 y otras citocinas pro inflamatorias (IL-1β, IL-6 y TNFα) en el plasma de pacientes con cáncer gástrico

“…Internacionalmente, la investigación se inclina a la búsqueda de biomarcadores que contribuyan a su detección con el propósito de mejorar su manejo. Se han estudiado los niveles de pepsinógenos , la proteína suPAR (Fernández-Botrán et al, 2020), la detección de ARN metilado (Abbasi & Heydari, 2018) y la detección y cuantificación de citocinas, la cual es prominente en el microambiente tumoral. Estudios recientes han identificado que las citocinas pueden ser detectadas a nivel plasmático siendo representativas de los niveles encontrados en el microambiente tumoral, tanto en pacientes con lesiones gástricas pre malignas como malignas.…”

Correlation between polymorphism of TYMS gene and toxicity response to treatment with 5-fluoruracil and capecitabine

“…3 In recent times, numerous studies have attempted to identify predisposition factors of cancer. Genes have been identified whose reduced expression can act as biomarkers for the early diagnosis of tumors, such as Reprimo gene for gastric cancer, 4 or reduced cancer incidence such as biallelic expression of EXITS genes in females explains a portion of the reduced cancer incidence compared to males across a variety of tumor types. 5 The probability that an individual affected by these mutations will develop a tumor is variable and depends on various factors.…”