Sub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)

Hieta, Juha-Pekka; Kopra, Jaakko; Räikkönen, Heikki; Kauppila, Tiina J.; Kostiainen, Risto

doi:10.1021/acs.analchem.0c01597

Cited by 18 publications

(31 citation statements)

References 55 publications

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“…tissue sections). This is consistent with our earlier study showing similar spot-to-spot repeatability (RSD = 16.8%) with frozen tissue sections of rodent brain . The sampling of more geometrically complex trichome regions was more inconsistent.…”

Section: Resultssupporting

confidence: 92%

“…However, we recently showed that IRLA sampling can now ablate 20–40 μm diameter spots by using simple optics . We also demonstrated the use of this focusing method for MS imaging of rodent brain tissue sections with a 70 μm lateral resolution, showing that IR laser ablation APPI-MS (LAAPPI-MS) and laser ablation ESI (LAESI-MS) can now reach a new quality level in imaging rodent tissue . Thus, far, LAAPPI-MS has not been used to measure the contents of a single cell from tissue, whereas LAESI-MS has already shown its potential for single-cell analysis, for example, in a recent study with Allium cepa tissue …”

Section: Introductionmentioning

confidence: 99%

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Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

Hieta

Sipari

Räikkönen

et al. 2021

J. Am. Soc. Mass Spectrom.

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In this study, we show that infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) imaging with 70 μm lateral resolution allows for the analysis of Arabidopsis thaliana (A. thaliana) leaf substructures ranging from single-cell trichomes and the interveinal leaf lamina to primary, secondary, and tertiary veins. The method also showed its potential for depth profiling analysis for the first time by mapping analytes at the different depths of the leaf and spatially resolving the topmost trichomes and cuticular wax layer from the underlying tissues. Negative ion LAAPPI-MS detected many different flavonol glycosides, fatty acids, fatty acid esters, galactolipids, and glycosphingolipids, whose distributions varied significantly between the different substructures of A. thaliana leaves. The results show that LAAPPI-MS provides a highly promising new tool to study the role of metabolites in plants.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

Hieta

Sipari

Räikkönen

et al. 2021

J. Am. Soc. Mass Spectrom.

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“…MAG (18:1), cholesterol, GalCer (d18:1/C24:1), and GalCer (d18:1/ C24:2) were mainly distributed in the corpus callosum, internal callosum, and less abundant in thalamus. [51][52][53][54] Creatine mainly distributed in brain areas except corpus callosum and glutamic acid was found to distribute in brain areas except corpus callosum, internal callosum, and hypothalamus.…”

Section: Imaging Of Pristine Mouse Brain Tissuementioning

confidence: 93%

Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

et al. 2022

J Mass Spectrom

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Desorption electrospray ionization/post‐photoionization (DESI/PI) is a newly developed ionization method by the combination of DESI and post‐photoionization for the simultaneous imaging of polar and nonpolar compounds in biological tissues. Dopants are of great importance in DESI/PI for the enhancement of signal intensities through ion–molecule reactions. In this work, to evaluate the performance of dopants in DESI/PI, an efficient homogenate model was developed, and four kinds of dopants (toluene, chlorobenzene, bromobenzene, and anisole) were tested using homogenate of mouse brain tissue as target sample. The influences of the dopants on the signal enhancements of different compounds were explained reasonably by the ionization mechanism. Then, the dopants with their optimum volume contents were applied to the mass spectrometry imaging (MSI). For a comprehensive imaging of various compounds with different polarities, methanol/toluene/formic acid (7:3:0.1) was chosen as the best choice. Finally, the stronger quantitative ability of DESI/PI with toluene as dopant for a few compounds in mouse brain tissue was demonstrated.

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“…Laser ablation techniques have also been applied to the analysis of murine tissues. LAAPPI and LAESI were recently utilized in the imaging of rodent brains 116 . A new infrared laser focusing technique was coupled with ambient mass spectrometry and applied to the analysis of lipids and other metabolites in dissected mouse brain samples, achieving tissue imaging with sub‐100 μm spatial resolution.…”

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2020: An annual review

Rankin‐Turner

Heaney

2021

Analytical Science Advances

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Recent developments in mass spectrometry (MS) analyses have seen a concerted effort to reduce the complexity of analytical workflows through the simplification (or removal) of sample preparation and the shortening of run‐to‐run analysis times. Ambient ionization mass spectrometry (AIMS) is an exemplar MS‐based technology that has swiftly developed into a popular and powerful tool in analytical science. This increase in interest and demonstrable applications is down to its capacity to enable the rapid analysis of a diverse range of samples, typically in their native state or following a minimalistic sample preparation approach. The field of AIMS is constantly improving and expanding, with developments of powerful and novel techniques, improvements to existing instrumentation, and exciting new applications added with each year that passes. This annual review provides an overview of applications of AIMS techniques over the past year (2020), with a particular focus on the application of AIMS in a number of key fields of research including biomedical sciences, forensics and security, food sciences, the environment, and chemical synthesis. Novel ambient ionization techniques are introduced, including picolitre pressure‐probe electrospray ionization and fiber spray ionization, in addition to modifications and improvements to existing techniques such as hand‐held devices for ease of use, and USB‐powered ion sources for on‐site analysis. In all, the information provided in this review supports the view that AIMS has become a leading approach in MS‐based analyses and that improvements to existing methods, alongside the development of novel approaches, will continue across the foreseeable future.

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Sub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)

Cited by 18 publications

References 55 publications

Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

Applications of ambient ionization mass spectrometry in 2020: An annual review

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