Sub-lethal pharmaceutical hazard tracking in adult zebrafish using untargeted LC–MS environmental metabolomics

Sotto, Ryan De; Medriano, Carl Angelo; Cho, Yunchul; Kim, Hyuk; Chung, In‐Young; Seok, Kwang-Seol; Song, Kyung Guen; Hong, Seok Won; Park, Youngja; Kim, Sungpyo

doi:10.1016/j.jhazmat.2017.06.009

Cited by 59 publications

(16 citation statements)

References 57 publications

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“…According to their biological activity, these compounds can be classified in antibiotics, analgesics, anti-histamines, anti-inflammatories, etc. [ 40 ] Amongst the pharmaceuticals, the antibiotics have been broadly studied. It has to be highlighted that the discovery of antibiotics and their widespread use revolutionized human health after the Second World War.…”

Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

“…Most of the research papers on this topic have reported that the zebrafish health or/and its capacity to respond to environmental changes are affected following both acute and chronic exposure to antibiotics. The toxicological effects of single and mixed antibiotics in zebrafish were studied by proteomic [ 30 ] and transcriptome sequencing [ 31 , 32 ], examination of behavioral activities [ 28 , 33 , 34 ] and biomarkers [ 35 , 36 ], histopathological analysis of various tissues [ 30 , 37 ], fish lethality assays (LC 50 ) [ 22 , 38 , 39 ] and following the fish morphological changes [ 40 ]. Antibiotics were found to interfere with the bacterial communities of zebrafish gut and with fish development, causing malformations, such as hatching delay, shorter body length, yolk sac edema, curved body axis and pericardial edema [ 23 , 25 , 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

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Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

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Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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“…To analyze swimming behavior, zebrafish fed a normal diet were recorded for 5 min. The swimming pattern, active time (min), and distance moved (cm) were analyzed for each individual [ 33 , 34 ]. The distance moved was calculated frame by frame.…”

Section: Methodsmentioning

confidence: 99%

Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (Lepidium meyenii)

Ryu

Jeon

et al. 2021

Cells

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Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced muscle atrophy using zebrafish (Danio rerio), a vertebrate model, and assessed whether administration of Lepidium meyenii (maca) as a dietary supplement can prevent muscle atrophy. Changes in skeletal myofibers in zebrafish were evaluated after exposure to dexamethasone for different periods and at different concentrations. Under optimized conditions, zebrafish pre-fed with maca for 3 days were exposed to 0.01% dexamethasone for 1 h/day for 7 days. Thereafter, myofiber loss, damaged muscle contractile proteins, and abnormal exploratory behavior due to the structural and functional impairment of skeletal muscle associated with muscle atrophy were investigated using hematoxylin–eosin, immunofluorescence staining, and behavioral analyses. Our findings suggest that dexamethasone induces muscle atrophy in zebrafish, inhibiting exploratory behavior by inducing myofiber loss, inhibiting muscle contraction, and causing changes in endurance and velocity. Thus, the zebrafish model can be used to screen pharmaceutical agents and to study muscle atrophy. Furthermore, maca is a potential dietary supplement to prevent muscle atrophy, as it protects muscle fibers.

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“…This technique is very sensitive enabling analysis of small quantity of the sample. LC-MS time of flight (LC-MS-TOF) has been utilized extensively in metabolomics and proteomics approach using zebrafish [12, 71]. Finally, the small size of zebrafish causes difficulties in dissection and thus requires more effort and skill to extract the organs compared to other conventional animal models.…”

Section: Introductionmentioning

confidence: 99%

The promise of zebrafish as a model of metabolic syndrome

et al. 2019

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Metabolic syndrome is a cluster including hyperglycaemia, obesity, hypertension, and hypertriglyceridaemia as a result of biochemical and physiological alterations and can increase the risk of cardiovascular disease and diabetes. Fundamental research on this disease requires validated animal models. One potential animal model that is rapidly gaining in popularity is zebrafish (Danio rerio). The use of zebrafish as an animal model conveys several advantages, including high human genetic homology, transparent embryos and larvae that allow easier visualization. This review discusses how zebrafish models contribute to the development of metabolic syndrome studies. Different diseases in the cluster of metabolic syndrome, such as hyperglycaemia, obesity, diabetes, and hypertriglyceridaemia, have been successfully studied using zebrafish; and the model is promising for hypertension and cardiovascular metabolic-related diseases due to its genetic similarity to mammals. Genetic mutation, chemical induction, and dietary alteration are among the tools used to improve zebrafish models. This field is expanding, and thus, more effective and efficient techniques are currently developed to fulfil the increasing demand for thorough investigations.

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Sub-lethal pharmaceutical hazard tracking in adult zebrafish using untargeted LC–MS environmental metabolomics

Cited by 59 publications

References 57 publications

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (Lepidium meyenii)

The promise of zebrafish as a model of metabolic syndrome

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