Subcellular Disorders Associated with Autism Peripheral Blood

Abdelmegui, Nabila E.; Kourtian, Silva

doi:10.3923/ajbs.2012.221.239

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…Abdelmeguid and Kourtian [14] described for the first time, cellular and subcellular alterations in large number of erythrocytes, leukocytes, as well as, platelets in both autistic candidate and their mothers. The most important subcellular alteration was noticed among nuclear chromatin, nuclear membranes, as well as cellular organelles specifically mitochondria.…”

Section: Discussionmentioning

confidence: 99%

“…They also added that individuals with autism show lower cell energy and deficiency in the capacity of mitochondrial energy reserve that could lead to cognitive impairment, language deficits, and abnormal energy metabolism. Abdelmeguid and Kourtian [14] reported that autistic candidates and their mothers possess altered peripheral blood cells. They also stated that erythrocytes were altered collectively, and related this to a decreased oxygen capacity of erythrocytes in blood circulation including, circulation in epitomes of fetal brain.…”

Section: Introductionmentioning

confidence: 99%

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Serum of Mothers Having Autistic Children Induces Cerebellar Purkinje cell Alterations in Experimental Model: A Possible Cause of Autism

Kourtian¹

2015

Autism Open Access

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Autism is a brain developmental disorder characterized by impaired social interaction. Our aim is to assess the morphology of plasma cells of mothers having autistic children and to evaluate the effect of administration of serum obtained from mothers having autistic children on the cerebellar Purkinje neurons. Two groups were used: control group, where pregnant rats remained intact while others were injected intraperitoneally with serum of mothers with no history of autism. In the experimental group pregnant rats were intraperitoneally injected serum of autistic children's mothers. The Transmission Electron Microscope showed altered plasma cells of autistic children's mothers, where the heterochromatin material had inversed distribution in all nuclei examined in addition to other alterations. Also, modified Purkinje neurons were obtained in neonates of experimental group showing degeneration and atrophied dendrites. Recommended examination of peripheral blood is essential for early intervention.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serum of Mothers Having Autistic Children Induces Cerebellar Purkinje cell Alterations in Experimental Model: A Possible Cause of Autism

Kourtian¹

2015

Autism Open Access

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“…When a light beam illuminates on a label-free cell, its spatial scattered light carries its identity characteristics (volume, structures, components, et al). It has been proved cell's volume change has close relationship with many malignant diseases [16][17][18][19][20][21]. For example, when patients suffer from the acute, chronic, and immature lymphocytic leukemia, the number of immature lymphocytes in blood increases with varying degrees [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Compared with the average diameter 7.5 μ m of mature lymphocytes [ 18 ], immature lymphocytes increase to about 10-16 μ m. On the other hand, nucleus is another important indicator for cytopathologic diagnosis. It has been found that lymphocyte nuclei volumes of autism children decrease to 70% of healthy children [ 19 ], but for leukemia patients they become larger [ 20 , 21 ]. Therefore, finding out the relationships between scattering features and cell’s morphology plays an important role for label-free biological detections, which is rooted in light scattering inversion (LSI) problem.…”

Section: Introductionmentioning

confidence: 99%

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

et al. 2022

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Objective and Impact Statement. Distinguishing malignant lymphocytes from normal ones is vital in pathological examination. We proposed an inverse light scattering (ILS) method for label-free suspended lymphocytes with complex fine structures to identify their volumes for pathological state. Introduction. Light scattering as cell’s “fingerprint” provides valuable morphology information closely related to its biophysical states. However, the detail relationships between the morphology with complex fine structures and its scattering characters are not fully understood. Methods. To quantitatively inverse the volumes of membrane and nucleus as the main scatterers, clinical lymphocyte morphologies were modeled combining the Gaussian random sphere geometry algorithm by 750 reconstructed results after confocal scanning, which allowed the accurate simulation to solve ILS problem. For complex fine structures, the specificity for ILS study was firstly discussed (to our knowledge) considering the differences of not only surface roughness, posture, but also the ratio of nucleus to the cytoplasm and refractive index. Results. The volumes of membrane and nucleus were proved theoretically to have good linear relationship with the effective area and entropy of forward scattering images. Their specificity deviations were less than 3.5%. Then, our experimental results for microsphere and clinical leukocytes showed the Pearson product-moment correlation coefficients (PPMCC) of this linear relationship were up to 0.9830~0.9926. Conclusion. Our scattering inversion method could be effectively applied to identify suspended label-free lymphocytes without destructive sample pretreatments and complex experimental systems.

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Erythrocyte Deformability in Children With Autism Spectrum Disorder: Correlation With Clinical Features

et al. 2019

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Biomechanical properties of erythrocytes play an important role in health and disease. Deformability represents intrinsic property of erythrocytes to undergo deformation that is crucial for their passage through the narrow capillaries. The erythrocyte damage can lead to compromised tissue perfusion and consequently play a role in the pathogenesis of various diseases including neurological ones. Data available in databases indicate that erythrocytes in autism spectrum disorder (ASD) are altered. This may affect the clinical symptoms of ASD. The aim of our study was to determine erythrocyte deformability in 54 children with ASD and correlate it with clinical symptoms. We found significant negative correlation between erythrocyte deformability and score in C domain of the Autism Diagnostic Interview-Revised (ADI-R) diagnostic tool describing the measure of restrictive, repetitive, and stereotyped behaviors and interests, mainly observable in C1 and C2, but not in C3 and C4 subdomains. This supports the findings of other authors and suggest that behavioral domain C comprises of more subcategories with different underlying etiology. Our results also indicate that abnormalities in erythrocyte deformability may be involved in ASD pathomecha-nisms and contribute to its clinical manifestation. Further research is necessary to bring more data and identify erythrocyte deformability as prognostic biomarker in ASD.

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Subcellular Disorders Associated with Autism Peripheral Blood

Cited by 3 publications

References 3 publications

Serum of Mothers Having Autistic Children Induces Cerebellar Purkinje cell Alterations in Experimental Model: A Possible Cause of Autism

Serum of Mothers Having Autistic Children Induces Cerebellar Purkinje cell Alterations in Experimental Model: A Possible Cause of Autism

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

Erythrocyte Deformability in Children With Autism Spectrum Disorder: Correlation With Clinical Features

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