Subcellular distribution of native estrogen receptor α and β subtypes in cultured human lens epithelial cells

Cammarata, Patrick R.; Chu, Shaoyou; Moor, Andrea N.; Wang, Zhaohui; Yang, Shaohua; Simpkins, James W.

doi:10.1016/j.exer.2003.09.027

Cited by 76 publications

(51 citation statements)

References 35 publications

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“…Consistently, extranuclear localization of both ERα and ERβ has been indicated Milner et al, 2005;Herrick et al, 2006). In fact, increasing evidence has demonstrated that ERβ are mainly localized extranuclearly Cammarata et al, 2004;Chen et al, 2004a;Yang et al, 2004;Chen et al, 2005;Herrick et al, 2006). (Gustafsson, 1999).…”

Section: Mitochondrial Localization Of Erβmentioning

confidence: 60%

“…It also controls ion homeostasis and the synthesis of heme, lipids, amino acids, and nucleotide. We and several other laboratories have recently reported the localization of ERβ in mitochondria in varies cells, including rat primary neuron Chen et al, 2005;Mehra et al, 2005), rat primary cardiomyocyte , a murine hippocampal cell line (HT-22), neurons and glia in rat hippocampus (Milner et al, 2005;Herrick et al, 2006), human breast cancer lines (MCF-7, MCF-10F) (Chen et al, 2004a;Chen et al, 2005), immortal human breast epithelial cells (HBEC) (Chen et al, 2005), human lens epithelial cell lines (nHLE and HLE-B3) (Cammarata et al, 2004;Cammarata et al, 2005), human osteosarcoma cells (SaOS-2) (Solakidi et al, 2005b), hepatocarcinoma cells (HepG2) (Solakidi et al, 2005a), human sperm (Solakidi et al, 2005b), and periodontal ligament cells (Jonsson, 2007). Similar perinuclear punctate staining of ERβ has been reported in a murine mammary epithelial cell line (HC11) and human fetal cortical neurons (Fried et al, 2004;Helguero et al, 2005).…”

Section: Mitochondrial Localization Of Erβmentioning

confidence: 99%

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Estrogen actions on mitochondria—Physiological and pathological implications

Simpkins

Yang

Sarkar

et al. 2008

Molecular and Cellular Endocrinology

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Estrogens are potent neuroprotective hormones and mitochondria are the site of cellular life-death decisions. As such, it is not surprising that we and other have shown that estrogens have remarkable effects on mitochondrial function. Herein we provide evidence for a primary effect of estrogens on mitochondrial function, achieved in part by the import of estrogen receptor β (ERβ) into the mitochondria where it mediates a number of estrogen actions on this vital organelle. ERβ is imported into the mitochondria, through tethering to cytosolic chaperone protein and/or through direct interaction with mitochondrial import proteins. In the mitochondria, ERβ can affect transcription of critical mitochondrial genes through the interaction with estrogen response elements (ERE) or through protein-protein interactions with mitochondrially imported transcription factors. The potent effects of estrogens on mitochondrial function, particularly during mitochondrial stress, argues for a role of estrogens in the treatment of mitochondrial defects in chronic neurodegenerative diseases like Alzheimer's disease (AD) and Parkinson's disease (PD) and more acute conditions of mitochondrial compromise, like cerebral ischemia and traumatic brain injury.

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Section: Mitochondrial Localization Of Erβmentioning

confidence: 60%

Section: Mitochondrial Localization Of Erβmentioning

confidence: 99%

Estrogen actions on mitochondria—Physiological and pathological implications

Simpkins

Yang

Sarkar

et al. 2008

Molecular and Cellular Endocrinology

Self Cite

135

105

View full text Add to dashboard Cite

show abstract

“…Furthermore, ER was localized in mitochondria of cardiomyocytes (54), of endothelia (17), of HepG2 hepatocarcinoma and SaOS-2 osteosarcoma cells (43), of human sperm cells (44), and of human periodontal ligament cells (51). Although in some cell mitochondria the presence of both ERa and ERb has been shown (55,57), in most cases the predominant or sole receptor isoform is ERb (16,43). In HepG2 and SaOS-2 cells, ERb is found solely in mitochondria, whereas ERa is confined to the nucleus, enriched in nucleoli (43).…”

Section: The Detection Of Steroid and Thyroid Receptors In Mitochondriamentioning

confidence: 99%

“…Later, using immunotechniques, ER was detected in mitochondria of rat uterine and ovarian cells (53), of MCF-7 breast cancer cells (16,57), of cultured human lens epithelial cells (55), and of rat hippocampus and neuronal cells (56). Furthermore, ER was localized in mitochondria of cardiomyocytes (54), of endothelia (17), of HepG2 hepatocarcinoma and SaOS-2 osteosarcoma cells (43), of human sperm cells (44), and of human periodontal ligament cells (51).…”

Section: The Detection Of Steroid and Thyroid Receptors In Mitochondriamentioning

confidence: 99%

“…The purification of receptor proteins and the availability of respective antibodies permitted the application of immune techniques, that is Western blotting, immunofluorescence, confocal and immunogold electron microscopy, for the identification of glucocorticoid (GR), estrogen (ER), androgen (AR), and thyroid hormone (TR) receptors in mitochondria (Fig. 2, Table 1) (37,(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(66)(67)(68)(69).…”

Section: The Detection Of Steroid and Thyroid Receptors In Mitochondriamentioning

confidence: 99%

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Steroid and thyroid hormone receptors in mitochondria

2008

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SummaryReceptors for glucocorticoids, estrogens, androgens, and thyroid hormones have been detected in mitochondria of various cell types by Western blotting, immunofluorescence labeling, confocal microscopy, and immunogold electron microscopy. A role of these receptors in mitochondrial transcription, OXPHOS biosynthesis, and apoptosis is now being revealed. Steroid and thyroid hormones regulate energy production, inducing nuclear and mitochondrial OXPHOS genes by way of cognate receptors. In addition to the action of the nuclearly localized receptors on nuclear OXPHOS gene transcription, a parallel direct action of the mitochondrially localized receptors on mitochondrial transcription has been demonstrated. The coordination of transcription activation in nuclei and mitochondria by the respective receptors is in part realized by their binding to common trans acting elements in the two genomes. Recent evidence points to a role of the mitochondrial receptors in cell survival and apoptosis, exerted by genomic and nongenomic mechanisms. The identification of additional receptors of the superfamily of nuclear receptors and of other nuclear transcription factors in mitochondria increases their arsenal of regulatory molecules and further underlines the central role of these organelles in the integration of growth, metabolic, and cell survival signals.2008 IUBMB IUBMB Life, 60(4): [210][211][212][213][214][215][216][217][218][219][220][221][222][223] 2008

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Ultrastructural localization of estrogen receptor β immunoreactivity in the rat hippocampal formation

et al. 2005

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Several lines of evidence indicate that estrogen affects hippocampal synaptic plasticity through rapid nongenomic mechanisms, possibly by binding to plasma membrane estrogen receptors (ERs). We have previously shown that ERalpha immunoreactivity (ir) is in select interneuron nuclei and in several extranuclear locations, including dendritic spines and axon terminals, within the rat hippocampal formation (Milner et al., [2001] J Comp Neurol 429:355). The present study sought to determine the cellular and subcellular locations of ERbeta-ir. Coronal hippocampal sections from diestrus rats were immunolabeled with antibodies to ERbeta and examined by light and electron microscopy. By light microscopy, ERbeta-ir was primarily in the perikarya and proximal dendrites of pyramidal and granule cells. ERbeta-ir was also in a few nonprincipal cells and scattered nuclei in the ventral subiculum and CA3 region. Ultrastructural analysis revealed ERbeta-ir at several extranuclear sites in all hippocampal subregions. ERbeta-ir was affiliated with cytoplasmic organelles, especially endomembranes and mitochondria, and with plasma membranes primarily of principal cell perikarya and proximal dendrites. ERbeta-ir was in dendritic spines, many arising from pyramidal and granule cell dendrites. In both dendritic shafts and spines, ERbeta-ir was near the perisynaptic zone adjacent to synapses formed by unlabeled terminals. ERbeta-ir was in preterminal axons and axon terminals, associated with clusters of small, synaptic vesicles. ERbeta-labeled terminals formed both asymmetric and symmetric synapses with dendrites. ERbeta-ir also was detected in glial profiles. The cellular and subcellular localization of ERbeta-ir was generally similar to that of ERalpha, except that ERbeta was more extensively found at extranuclear sites. These results suggest that ERbeta may serve primarily as a nongenomic transducer of estrogen actions in the hippocampal formation.

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Subcellular distribution of native estrogen receptor α and β subtypes in cultured human lens epithelial cells

Cited by 76 publications

References 35 publications

Estrogen actions on mitochondria—Physiological and pathological implications

Estrogen actions on mitochondria—Physiological and pathological implications

Steroid and thyroid hormone receptors in mitochondria

Ultrastructural localization of estrogen receptor β immunoreactivity in the rat hippocampal formation

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