Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt^II Metallacycle

Abstract

Metal-driven self-assembly afforded am ultitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts,h ost-guest, and stimuli-responsive systems.H owever,t he interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in-cell tracking of aP t 2 L 2 metallacycle.W es howt hat our hexagonal supramolecule accumulates in cancer cell nuclei, exerting adistinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4-richr egions. SCC co-localizes with epitopes of the quadruplex-specific antibody BG4 and replaces other well-known G4 stabilizers. Moreover,the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly,allowing its subcellular interaction tracking.

Design and Applications of Water-Soluble Coordination Cages

“…Peinador, Quintela, and co-workers prepared Pd II and Pt II water-soluble metallacycles and 2D receptors using this approach. 83 − 85 Mukherjee and co-workers presented water-soluble “molecular dice” ( 56 ) synthesized via coordination-driven self-assembly of flexible, cationic, tritopic ligand 55 ( Figure 15 a). 86 Likewise, Sun and co-workers reported the self-assembly of photochromic, redox-capable nanocapsule 58 made of four cis -blocked palladium corners and two pyridinium-functionalized tetratopic ligands ( 57 ), which contain two tris(4-pyridyl)-1,3,5-triazine bridged by a p -xylene linker ( Figure 15 b).…”

Ruthenium–arene complexes bearing naphthyl-substituted 1,3-dioxoindan-2-carboxamides ligands for G-quadruplex DNA recognition

“…In recent years, we developed different classes of metalbased compounds to selectively target G-quadruplexes, including Ni(II) salen-type derivatives, 26 Pt(II) supramolecular coordination complexes, 27,28 and Ru(II) N-heterocyclic carbenes. 29,30 The aim of this article is to explore a new strategy for the design of Ru-arene complexes possessing distinct G4 binding abilities that can positively affect their anticancer activity.…”