Subcellular localization and identification of acyl-CoA: lysophosphatidylethanolamine acyltransferase (LPEAT) in the arachidonic acid-rich green microalga, Myrmecia incisa Reisigl

Heterologous expression of MiMLDP, a major lipid droplet protein of the microalga Myrmecia incisa, in Saccharomyces cerevisiae promotes more triacylglycerol storage principally by enlarging lipid droplet size

“…Immunoprecipitation (IP) approach was used to obtain the protein which could be immunologically reacted with the anti-MiMLDP polyclonal antibody from M. incisa as described by Liu et al (2022). Five microliters of the puri ed anti-MiMLDP polyclonal antibody and 150 µL of crude extracts containing native MiMLDP from this microalga were mixed with 200 µL of IP lysis and wash buffer (Pierce ™ Classic IP Kit, Thermo Scienti c) for incubation at 4°C for 12 h to produce immune complex.…”

“…An Easy-nLC 1200 system (Thermo Scienti c) was used to analyze the digested peptide sequences as described in detail by Liu et al (2022). The separated peptides were analyzed using a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scienti c) in data-dependent acquisition mode.…”