Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes

Yonekura, Shinichi; Hirota, Shohei; Miyazaki, Honami; Tokutake, Yukako

doi:10.1080/10495398.2015.1102148

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…We found that FABP4 was expressed in the cytoplasm during the preadipocyte stage, and it gradually transferred from the cytoplasm to the nucleus with the adipocyte differentiation, expressed in both the nucleus and the cytoplasm to some extent ( Figure 3 B,C). This result is consistent with the findings of Yonekura et al and Ayers et al [ 19 , 20 ]. Interestingly, the KDM2A protein was always located in the nucleus, whether in preadipocytes or in mature adipocytes at the stage of terminal differentiation ( Figure 3 B,C).…”

Section: Resultssupporting

confidence: 94%

Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation

Hua

Yue

Zhao

et al. 2021

IJMS

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Epigenetic signals and chromatin-modifying proteins play critical roles in adipogenesis, which determines the risk of obesity and which has recently attracted increasing interest. Histone demethylase 2A (KDM2A) is an important component of histone demethylase; however, its direct effect on fat deposition remains unclear. Here, a KDM2A loss of function was performed using two unbiased methods, small interfering RNA (siRNA) and Cre-Loxp recombinase systems, to reveal its function in adipogenesis. The results show that the knockdown of KDM2A by siRNAs inhibited the proliferation capacity of 3T3-L1 preadipocytes. Furthermore, the promotion of preadipocyte differentiation was observed in siRNA-treated cells, manifested by the increasing content of lipid droplets and the expression level of adipogenic-related genes. Consistently, the genetic deletion of KDM2A by Adipoq-Cre in primary adipocytes exhibited similar phenotypes to those of 3T3-L1 preadipocytes. Interestingly, the knockdown of KDM2A upregulates the expression level of Transportin 1(TNPO1), which in turn may induce the nuclear translocation of PPARγ and the accumulation of lipid droplets. In conclusion, the ablation of KDM2A inhibits preadipocyte proliferation and promotes its adipogenic differentiation. This work provides direct evidence of the exact role of KDM2A in fat deposition and provides theoretical support for obesity therapy that targets KDM2A.

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Section: Resultssupporting

confidence: 94%

Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation

Hua

Yue

Zhao

et al. 2021

IJMS

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“…In mammals, the differentiation of intramuscular preadipocytes has been well studied, especially in bovine and porcine. Previous studies identified FTO [ 5 ], GPR39 [ 7 ], myostatin [ 6 ], microRNA-143 [ 8 ], PPARG [ 9 ], FABP4 [ 10 ], and LIPE [ 11 ] as key roles in the regulation of intramuscular preadipocyte differentiation and intramuscular fat deposition in pig and cattle. However, the mechanism by which intramuscular fat deposition is regulated in chicken is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Analysis of long noncoding RNA and mRNA using RNA sequencing during the differentiation of intramuscular preadipocytes in chicken

Zhang

Han

et al. 2017

PLoS ONE

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Long noncoding RNAs (lncRNAs) regulate metabolic tissue development and function, including adipogenesis. However, little is known about the function and profile of lncRNAs in intramuscular preadipocyte differentiation in chicken. Here, we identified lncRNAs in chicken intramuscular preadipocytes at different differentiation stages using RNA sequencing. A total of 1,311,382,604 clean reads and 25,435 lncRNAs were obtained from 12 samples. In total, 7,433 differentially expressed genes (4,698 lncRNAs and 2,735 mRNAs) were identified by pairwise comparison. These 7,433 differentially expressed genes were grouped into 11 clusters based on their expression patterns by K-means clustering. Using Weighted Gene Coexpression Network Analysis, we identified four stage-specific modules positively related to I0, I2, I4, and I6 stages and two stage-specific modules negatively related to I0 and I2 stages, respectively. Many well-known and novel pathways associated with intramuscular preadipocyte differentiation were identified. We also identified hub genes in each stage-specific module and visualized them in Cytoscape. Our analysis revealed many highly-connected genes, including XLOC_058593, BMP3, MYOD1, and LAMP3. This study provides a valuable resource for chicken lncRNA study and improves our understanding of the biology of preadipocyte differentiation in chicken.

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“…At d 92 of TOC, there was up-regulation of fatty acid binding protein 4 (FABP4, 4-fold change) and fatty acid elongase 6 (ELOVL6; 8-fold change) mRNA expression, and down-regulation of mRNA involved in lipolysis (PLIN1 and PLIN5) and adipogenesis (SCAP) compared to d 0. FABP4 transports intracellular fatty acids to the nucleus where it alters transcription of certain genes (37). FABP4 expression is up-regulated during adipocyte differentiation (38) and serves as a marker of differentiation in bovine adipocytes (37,39).…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNA Transcriptome Involved in Bovine Intramuscular Fat Deposition

Duckett

Greene

2022

Front. Vet. Sci.

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BackgroundIntramuscular fat deposition in beef is a major determinant of carcass quality and value in the USA. The objective of this study was to examine changes in microRNA (miRNA) transcriptome that are involved with intramuscular fat deposition with time-on-concentrates (TOC). Yearling steers were individually fed a high concentrate diet and changes in intramuscular fat deposition were monitored by real-time ultrasound at 28 to 33 d intervals. Longissimus muscle biopsies collected on d 0, 92 and 124 TOC to examine changes in miRNA transcriptome that are involved in intramuscular fat deposition.ResultsSteer body weight increased (P < 0.0001) at each weigh day during TOC. Fat thickness increased (P < 0.005) from d 28 to 124. Ribeye area was larger (P < 0.001) on d 124 than d 61, which was larger than d 0 and 28. Ultrasound intramuscular fat content was greater (P < 0.001) on d 92 and 124 compared to d 0, 28 or 61. Sequencing of the muscle biopsy samples identified one miRNA, bta-miR-122, that was up-regulated (P < 0.005) at d 92 and 124 compared to d 0. At d 92 TOC, mRNA expression levels of fatty acid binding protein 4 (FABP4) and elongase 6 (ELOVL6) were up-regulated (P < 0.01) compared to d 0; whereas at d 124, lipogenic genes involved in de novo fatty acid synthesis, fatty acid transport, elongation and desaturation were highly up-regulated compared to d0.ConclusionsSmall RNA sequencing identified bta-miR-122 as a potential miRNA of interest that may be involved in intramuscular fat deposition with increasing TOC. Increased intramuscular fat content, as measured by real-time ultrasound, combined with differential gene expression suggests that preadipocyte differentiation may be stimulated first, which is followed by a global up-regulation of lipogenic genes involved in de novo fatty acid synthesis that provide fatty acids for subsequent hypertrophy.

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Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes

Cited by 10 publications

References 20 publications

Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation

Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation

Analysis of long noncoding RNA and mRNA using RNA sequencing during the differentiation of intramuscular preadipocytes in chicken

Identification of microRNA Transcriptome Involved in Bovine Intramuscular Fat Deposition

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