Subcellular Organisation in Bacteria

Lewis, Peter J.

doi:10.1007/978-3-540-74921-9_1

Cited by 3 publications

(8 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such a cell model (Fig. 5) is based on recent progress in the understanding of subcellular structuring of prokaryotes, where nucleoid/ribosome/cell envelope connections synchronize gene expression, chromosome orientation, replication, and segregation with cell septation and division during the cell cycle (14,15,24,28,34,70,73,80,98,120,121,193,225,235,255). The nucleoid excrescences and their anchoring at the cytoplasmic side of the cell envelope are qualitatively consistent with the subdiffusive motion (non-Brownian) of chromosomal loci (241).…”

mentioning

confidence: 75%

“…cell in toto (74,87,107,110,120,140,246,247,259) as it progresses through the cell cycleperhaps starting at a 1-s resolution for one division per hour and then increasing the temporal resolution toward milliseconds and microseconds while zooming in on important phenomena and regions of the cell. Such a model could summarize current knowledge of the flow of physicochemical information from the nutrient solution and its transformation through the cell envelope to activate and maintain gene expression and genome replication, leading to cell volume growth and eventual cell fission.…”

Section: Discussionmentioning

confidence: 99%

“…As a common feature, the prokaryotic and eukaryotic cytoskeleton proteins utilize ionic ATP and/or GTP, which points to electrostatic interactions participating in active (energy-requiring) mechanisms to effect structuring functions (28,66); for example, ATP-binding sites are required for proper cell division (5). Thus, at the subcellular and suborganelle levels (ϳ50 to 500 nm) of crowded and interacting biomacromolecules, the distinction between prokaryotic and eukaryotic cells is beginning to be erased (60,120,125,153).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

From Water and Ions to Crowded Biomacromolecules:In VivoStructuring of a Prokaryotic Cell

Spitzer¹

2011

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

SUMMARY The interactions and processes which structure prokaryotic cytoplasm (water, ions, metabolites, and biomacromolecules) and ensure the fidelity of the cell cycle are reviewed from a physicochemical perspective. Recent spectroscopic and biological evidence shows that water has no active structuring role in the cytoplasm, an unnecessary notion still entertained in the literature; water acts only as a normal solvent and biochemical reactant. Subcellular structuring arises from localizations and interactions of biomacromolecules and from the growth and modifications of their surfaces by catalytic reactions. Biomacromolecular crowding is a fundamental physicochemical characteristic of cells in vivo . Though some biochemical and physiological effects of crowding (excluded volume effect) have been documented, crowding assays with polyglycols, dextrans, etc., do not properly mimic the compositional variety of biomacromolecules in vivo . In vitro crowding assays are now being designed with proteins, which better reflect biomacromolecular environments in vivo , allowing for hydrophobic bonding and screened electrostatic interactions. I elaborate further the concept of complex vectorial biochemistry, where crowded biomacromolecules structure the cytosol into electrolyte pathways and nanopools that electrochemically “wire” the cell. Noncovalent attractions between biomacromolecules transiently supercrowd biomacromolecules into vectorial, semiconducting multiplexes with a high (35 to 95%)-volume fraction of biomacromolecules; consequently, reservoirs of less crowded cytosol appear in order to maintain the experimental average crowding of ∼25% volume fraction. This nonuniform crowding model allows for fast diffusion of biomacromolecules in the uncrowded cytosolic reservoirs, while the supercrowded vectorial multiplexes conserve the remarkable repeatability of the cell cycle by preventing confusing cross talk of concurrent biochemical reactions.

show abstract

mentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

From Water and Ions to Crowded Biomacromolecules:In VivoStructuring of a Prokaryotic Cell

Spitzer¹

2011

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…In fast‐growing cells, RNAPs are redistributed with DNA‐binding proteins at oriC region into one to four subregions of the nucleoid called transcription foci where active transcription of rRNA genes preferentially occurs (Cabrera & Jin, 2006). Transcription foci in B. subtilis and E. coli are similar in distribution and appearance (Lewis, 2008).…”

Section: Cell Component Inventorymentioning

confidence: 99%

An inventory of the bacterial macromolecular components and their spatial organization

Vendeville¹,

Larivière²,

Fourmentin³

2011

FEMS Microbiol Rev

View full text Add to dashboard Cite

Formerly regarded as small 'bags' of nucleic acids with randomly diffusing enzymes, bacteria are organized by a sophisticated and tightly regulated molecular machinery. Here, we review qualitative and quantitative data on the intracellular organization of bacteria and provide a detailed inventory of macromolecular structures such as the divisome, the degradosome and the bacterial 'nucleolus'. We discuss how these metabolically active structures manage the spatial organization of the cell and how macromolecular crowding influences them. We present for the first time a visualization program, lifeexplorer, that can be used to study the interplay between metabolism and spatial organization of a prokaryotic cell.

show abstract

“…Semakin banyak total BAL maka semakin tinggi total padatan yang dihasilkan. Lewis (2008) menyatakan bakteri memiliki membran sel yang tersusun protein dan lemak, sehingga semakin banyak jumlah koloni BAL dalam media fermentasi maka total padatan yang diperoleh akan semakin banyak. Selain itu, total padatan juga berkaitan erat dengan pH yang dihasilkan.…”

Section: Total Padatanunclassified

Perbandingan Susu Kambing dan Susu Kedelai dalam Pembuatan Kefir

Rossi

Hamzah

Febriyani

2016

JPI

View full text Add to dashboard Cite

ABSTRAKTujuan dari penelitian ini adalah untuk mendapatkan rasio terbaik susu kambing dengan susu kedelai untuk menghasilkan kualitas kefir tertinggi. Rancangan Acak Lengkap (RAL) digunakan dalam penelitian ini dengan lima perlakuan dan empat ulangan. Perlakuannya adalah MS1 (susu kambing 100: susu kedelai 0), MS2 (susu kambing 75: susu kedelai 25), MS3 (susu kambing 50: susu kedelai 50), MS4 (susu kambing 25: susu kedelai 75), dan MS5 (susu kambing 0: susu kedelai 100). Parameter yang diamati adalah tingkat keasaman (pH), total bakteri asam laktat, padatan total dan kadar alkohol. Hasil penelitian menunjukkan bahwa rasio susu kambing dan susu kedelai berpengaruh nyata (P <0,05) terhadap pH, jumlah bakteri asam laktat, padatan total dan kadar alkohol. Perlakuan terbaik dalam penelitian ini adalah MS2.Kata kunci: pembuatan kefir, susu kambing, susu kedelai ABSTRACT The study investigated the best ratio of goat's milk with soya milk proportion in producing the highest quality of kefir. A Completely Randomized Design (CRD) was followed with five treatments and four replications. The treatments consisted of MS1 (goat

show abstract

Subcellular Organisation in Bacteria

Cited by 3 publications

References 148 publications

From Water and Ions to Crowded Biomacromolecules:In VivoStructuring of a Prokaryotic Cell

From Water and Ions to Crowded Biomacromolecules:In VivoStructuring of a Prokaryotic Cell

An inventory of the bacterial macromolecular components and their spatial organization

Perbandingan Susu Kambing dan Susu Kedelai dalam Pembuatan Kefir

Contact Info

Product

Resources

About