Subchondral bone micro-architectural alterations in osteoarthritis: a synchrotron micro-computed tomography study

Chappard, Christine; Peyrin, Françoise; Bonnassie, A.; Lemineur, G.; Brunet-Imbault, B.; Lespessailles, Éric; Benhamou, Claude Laurent

doi:10.1016/j.joca.2005.09.008

Cited by 144 publications

(132 citation statements)

References 49 publications

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“…These changes are in contrast to those found in human end-stage OA. (4,5,16) However, changes of subchondral bone in experimental guinea pig models at a more advanced stage were found comparable with those in human end-stage OA. (17,18) This suggests that bone remodeling in preclinical animal models of OA is biphasic: an early decrease in trabecular bone volume followed by a phase in which the subchondral bone becomes denser and stiffens.…”

Section: Introductionmentioning

confidence: 66%

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Intema

Sniekers

Weinans

et al. 2010

Journal of Bone and Mineral Research

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In osteoarthritis (OA), cartilage degradation is accompanied by subchondral bone changes. The pathogenesis and physiology of bone changes in OA are still unclear. The changes in subchondral bone architecture and cartilage damage were compared in differently induced experimental models of OA. Experimental OA was induced bilaterally by anterior cruciate ligament transection (ACLT) or by cartilage trauma (Groove model); bilateral sham surgery served as control. Lysylpyridinoline (LP, bone resorption) and C-telopeptide of type II collagen (CTX-II, cartilage breakdown) were measured over time. At 20 weeks after surgery, the subchondral cortical plate and trabecular bone of the tibia were analyzed by micro-computed tomography (mCT) and cartilage degeneration was analyzed histologically and biochemically. In both models, cartilage degeneration and cortical subchondral plate thinning were present. CTX-II levels were elevated over time in both models. Subchondral trabecular bone changes were observed only in the ACLT model, not in the Groove model. Correspondingly, LP levels were elevated over time in the ACLT model and not in the Groove model. Interestingly, the trabecular bone changes in the ACLT model were extended to the metaphyseal area. The early decrease in plate thickness, present in both models, as was cartilage damage, suggests that plate thinning is a phenomenon that is intrinsic to the process of OA independent of the cause/induction of OA. On the other hand, trabecular changes in subchondral and metaphyseal bone are not part of a common pathway of OA development and may be induced biomechanically in the destabilized and less loaded ACLT joint. ß

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Section: Introductionmentioning

confidence: 66%

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Intema

Sniekers

Weinans

et al. 2010

Journal of Bone and Mineral Research

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“…The previous study by Chappard and colleagues investigated 6 hip OA patients. (10) More studies are needed in the future. In addition, all the bone samples in this study were terminal-staged OA, and we could not study early-staged OA or normal controls.…”

Section: Discussionmentioning

confidence: 99%

“…In that study, an increased trabecular thickness and a decreased degree of mineralization was observed in subchondral bone. (10) We hypothesized that bone metabolism changes in OA subchondral bone are caused by bone structural changes such as trabecular bone thickening and bone cyst formation, and that subchondral bone turnover is correlated with subchondral bone structure. Resolution of clinical CT and magnetic resonance imaging (MRI) has improved significantly in recent years, and the subchondral bone structure of OA patients can now be analyzed more finely and quantitatively.…”

Section: Introductionmentioning

confidence: 99%

Relationship between microstructure and degree of mineralization in subchondral bone of osteoarthritis: A synchrotron radiation µCT study

Chiba

Nango²,

Kubota³

et al. 2012

Journal of Bone and Mineral Research

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We analyzed the microstructure and degree of mineralization of the subchondral trabecular bone in hip osteoarthritis (OA) using synchrotron radiation computed tomography (SRCT) to identify the relationship between bone structure and bone turnover. Subchondral bone samples were extracted from femoral heads of 10 terminal-staged hip OA patients. The SRCT scan was performed at 30 keV energy and 5.9 mm voxel size. Trabecular bone structure, bone cyst volume, and the degree of trabecular bone mineralization were measured, and correlations between bone structure and the degree of mineralization were analyzed. In addition, the trabecular bone was divided into the area immediately surrounding the bone cyst and the remaining area, and they were compared. The average cyst volume fraction in the whole region was 31.8%, and the bone volume fraction in the bone region was 55.6%. Cyst volume was the only structural parameter that had a significant correlation with the degree of mineralization. The degree of mineralization was diminished when the bone cyst was larger (r ¼ À0.81, p ¼ 0.004). The trabecular bone immediately surrounding the bone cyst had a lower degree of mineralization when compared with the remaining trabecular bone (p ¼ 0.008). In the bone sclerosis of OA subchondral bone, there are many large and small bone cysts, which are expected to play a significant part in the high bone turnover of OA. ß

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“…Voxel sizes less than 10 m are readily achievable with Micro-CT (Guldberg et al, 2003), which is superior to those currently achievable with ultrasound (30 m) and magnetic resonance imaging (100 m). Micro-CT has mainly been used for visualizing and quantifying bone architecture and development (Guldberg et al, 2004), and several studies have been conducted using this technique to quantify trabecular structures (Chappard et al, 2006). More recent studies using radio-opaque contrast agents have enabled the visualization of microvascular structures, such as those associated with bone fracture healing and coronary artery vasa vasorum (Gossl et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Quantitative volumetric analysis of cardiac morphogenesis assessed through micro‐computed tomography

Butcher

Sedmera

Guldberg

et al. 2006

Developmental Dynamics

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We present a method to generate quantitative embryonic cardiovascular volumes at extremely high resolution without tissue shrinkage using micro-computed tomography (Micro-CT). A CT dense polymer (Microfil, Flow Tech, Inc.) was used to perfuse avian embryonic hearts from Hamburger and Hamilton stage (HH) 15 through HH36, which solidified to create a cast within the luminal space. Hearts were then scanned at 10.5 m 3 voxel resolution using a VivaCT scanner, digital slices were contoured for regions of interest, and computational analysis was conducted to quantify morphogenetic parameters. The three-dimensional morphology was compared with that of scanning electron microscopy (SEM) images and serial section reconstruction of similarly staged hearts. We report that Microfil-perfused hearts swelled to maximum end-diastolic volume with negligible shrinking after polymerization. Comparison to SEM revealed good agreement of cardiac chamber proportions and intracardiac tissue structures (i.e., valves and septa) at the stages of development assessed. Quantification of changes in chamber volume over development revealed several notable results that confirm earlier hypotheses. Heart chamber volumes grow over two orders of magnitude during the 1-week developmental period analyzed. The atrioventricular canal comprised a significant proportion of the early heart volume. While left atrium/left ventricular volume ratios approached 1 in later development, right atrium/right ventricle ratios increase to over 2.5. Quantification of trabeculation patterns confirmed that the right and left ventricles are similarly trabeculated before HH27, after which the right ventricle became quantitatively coarser than that of the left ventricle. These results demonstrate that Micro-CT can be used to image and quantify cardiovascular structures during development. Developmental Dynamics 236:802-809, 2007.

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Subchondral bone micro-architectural alterations in osteoarthritis: a synchrotron micro-computed tomography study

Cited by 144 publications

References 49 publications

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Relationship between microstructure and degree of mineralization in subchondral bone of osteoarthritis: A synchrotron radiation µCT study

Quantitative volumetric analysis of cardiac morphogenesis assessed through micro‐computed tomography

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