Subpicosecond dynamics in DNA from leaves ofin vitro-grown apple plants: A SERS study

Abstract

Abstract. In this work the SERS total half bandwidths of five genomic DNAs from in vitro-grown apple leaf tissues (Malus domestica Borkh., Fam Rosaceae, cvs. Florina, Rebra, Goldrush, Romus 3 and Romus 4) have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure, on the type of genomic apple plant DNA and on time, are reported. An example of the time-dependence of SERS band parameters of DNA, in the proximity of silver nanoparticles, has been given. It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs from in vitro-grown apple leaf tissues can be monitored with surface-enhanced Raman spectroscopy.Particularly O)] of genomic DNAs from apple leaves are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs from in vitro-grown apple leaf tissues are typically in the wavenumber range from 14 to 52 cm −1 . Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.76 ps and larger than 0.20 ps.A comparison between different ranges of FT-Raman and SERS band parameters of DNA extracted from leaf tissues, respectively, is given.We have found that the bands of DNA from Romus 3 and Rebra cultivars are suitable for studying the dynamical behaviour of molecular subgroups, in genomic nucleic acids extracted from in vitro-grown apple plants.

Molecular relaxation processes in genomic DNA from leaf tissues: A surface-enhanced Raman spectroscopic study

“…A confocal Raman microspectroscopic study into the vibrational half bandwidths of molecular subgroups in calf-thymus DNA at pH 7 and pH 3, in the presence of Na + , Ca 2+ and Mg 2+ ions, respectively, was previously reported by us [5]. The bandwidths in the Raman spectra were sensitive to a dynamics active on a time scale ranging from 0.34 to 1.44 ps [5,[7][8][9].…”

“…Studies of relaxation processes in liquids are valuable in providing information about the intermolecular interactions in condensed matter [3]. Raman and infrared spectroscopy offer the most significant information concerning the behaviour of a condensed system on the picosecond timescale [2,9]. Among the techniques available for the study of molecular motions in liquids, Raman scattering has the distinct advantage, that it enables simultaneous analyses of both reorientational and vibrational processes ( [2,3,7,8] and references therein).…”

“…The macromolecular motion in fluids is generally too slow to be observed in the Raman time window that is accessible in the frequency domain. On contrary, the motion of molecular subgroups can be fast enough [8,9,15].…”

“…Molecular relaxation processes in calf-thymus DNA, in the presence of different concentrations of Mn 2+ and Na + ions, respectively, were investigated by Raman spectroscopy [6,9,13,14]. The full widths at half-maximum (FWHMs) for the bands of DNA were typically in the wavenumber range from 9 to 33.5 cm −1 .…”

Analysis of Biomolecules Based on the Surface Enhanced Raman Spectroscopy

“…Results showed that the SERS method could be applied to studying the dynamics of DNA approaching the surface of the metallic substrate, with good perspectives of analyzing interactions of DNA-ligand or changes of DNA structure under environmental stress conditions [ 120 , 121 ]. They extended the application of this SERS method to other plant leaves, such as chrysanthemum, common sundew, edelweiss and so on [ 122 ]. The SERS spectra of genomic DNAs from tomato plants was also collected and the structural changes of DNAs undergoing cryopreservation were discussed [ 123 ].…”