Substance P and 5-hydroxytryptamine immunoreactive presynaptic boutons on presumed .ALPHA.-motoneurons in the chicken ventral horn.

Atsumi, Saoko; Sakamoto, Hiroshi; Yokota, Sadaki; Fujiwara, Toshiyuki

doi:10.1679/aohc.48.159

Cited by 31 publications

(5 citation statements)

References 34 publications

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“…All three neurotransmitters have been localised, by electronmicroscopy, within boutons making close synaptic contact with ventral horn motoneurones (Atsumi et al, 1985;Ulfhake et al, 1987) consistent with the proposal that these neurones regulate spinal motor output. When applied iontophoretically to spinal motoneurones TRH has similar electrophysiological effects to substance P and 5-HT, facilitating the activation of motoneurones produced by the excitatory amino acids glutamate or aspartate (White, 1985).…”

supporting

confidence: 79%

Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Fone

Johnson

Bennett

et al. 1989

British J Pharmacology

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1 The behavioural effects of the intrathecal injection of a thyrotrophin-releasing hormone (TRH) analogue L-orotyl-L-histidyl-prolineamide (CG 3509, 0.5 /g), the non-selective 5-HT, and 5-HT2 receptor agonist 5-methoxy-N,N'-dimethyltryptamine (5-MeODMT, 2-100pg) and the selective 5-HT2 receptor agonist 2,5-dimethoxy-a,4-dimethyl-benzene ethamine hydrochloride (DOM, 2-25 ug) were compared with the response of systemically administered 5-MeODMT (2 mg kg-1, i.p.) in rats, to establish whether the agonist-induced behaviours were mediated by bulbospinal 5-HT, or 5-HT2 receptors. 2 Intrathecal injection of 5-MeODMT or DOM produced dose-related back muscle contractions (a previously undocumented behaviour) and wet-dog shakes which were both markedly attenuated by ritanserin pretreatment (1 mg kg 1, i.p.) indicating the involvement of 5-HT2 receptors. In contrast, reciprocal forepaw treading, flat body posture and Straub-tail were evoked by 5-MeODMT but not by DOM indicating that these behaviours were not produced by 5-HT2 receptor activation alone. However, as ritanserin pretreatment reduced the reciprocal forepaw treading induced by intrathecal 5-MeODMT, this behaviour may be facilitated by 5-HT2 receptor activation.3 Intrathecal 5,7-dihydroxytryptamine (5,7-DHT, 2 x 150pg) treatment decreased thoraco-lumbar spinal cord 5-HT (-95%) and potentiated the back muscle contractions produced by intrathecal DOM injection without altering the wet-dog shake behaviour. None of the components of the 5-HT syndrome produced by 5-MeODMT (2mgkg-1, i.p.), with the exception of a small increase in wet-dog shakes, was significantly altered by intrathecal 5,7-DHT (which reduced thoraco-lumbar spinal cord 5-HT by 84%). Taken together these data suggest that the only 5-HT agonist-induced behaviour mediated by the activation of 5-HT2 receptors located postsynaptic to bulbospinal 5-hydroxytryptaminergic (5-HTergic) neurones was back muscle contractions. 4 The wet-dog shake and forepaw licking behaviors produced by intrathecal CG 3509 (0.5 Mg) were attenuated when ritanserin was administered intrathecally 30 min before, but not when it was given at the same time as CG 3509 and neither behaviour was altered by intrathecal 5,7-DHT. This suggests that bulbospinal 5-HTergic neurones are not involved in the production of these TRH analogue-induced behaviours and that the 5-HT2 receptors which mediate these behaviours are not located in the spinal cord.

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supporting

confidence: 79%

Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Fone

Johnson

Bennett

et al. 1989

British J Pharmacology

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“…By contrast, in the ventral horn, SPLI was not detected in cell bodies but only in fibers. Electronmicroscopic studies in various species have revealed that these fibers make synaptic contacts with spinal motoneurones and regulate their functions (de Lanerolle and LaMotte, 1982;Vacca etal., 1982;Atsumi et al, 1985;Ulfhake etal., 1987;Wang etal., 1989).…”

Section: Discussionmentioning

confidence: 99%

Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Taquet

Plachot

Pohl

et al. 1992

J. Neural Transmission

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Possible changes in neuropeptides within the ventral horn of the spinal cord were investigated after unilateral dorsal rhizotomy at the lumbar level (L1-L6) in adult rats. Ten days after the surgery, immunohistochemical observations and radioimmunological determinations confirmed a marked loss of calcitonin gene-related peptide (CGRP)- and substance P (SP)-like immunoreactivities within the superficial layers of the deafferented dorsal horn, as expected from the degeneration of primary afferent fibres containing these peptides. A concomitant increase in immunohistochemical staining and levels of CGRP (+296%) and CCK (+71%)-like immunoreactivities was observed in the ipsilateral ventral horn where both peptides are located in motoneurones. In contrast, substance P-like immunoreactivity that is confined to fibres and terminals within the ventral horn, was not altered by dorsal rhizotomy. These data indicate that the expression of neuropeptides in spinal motoneurones can be influenced by primary afferent inputs.

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“…It has been shown that 5-HT-containing nerve terminals are present in the ventral horn of the spinal cord (Dahlstr6m & Fuxe, 1965;Steinbusch, 1981) in close contact with motoneurones (Atsumi, Sakamoto, Yokota & Fujiwara, 1985). The serotonergic projection derives from the raphe nuclei and the medial reticular formation of the medulla (Dahlstr6m & Fuxe, 1965).…”

Section: Introductionmentioning

confidence: 99%

Direct excitation of rat spinal motoneurones by serotonin.

Takahashi

Berger

1990

The Journal of Physiology

192

105

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SUMMARY1. The effects of serotonin (5-HT) on visually identified motoneurones were investigated using the whole-cell recording technique in a neonatal rat spinal cord slice preparation.2. In current-clamp recordings, bath application of 5-HT depolarized motoneurones. This effect was observed after synaptic inputs were abolished by replacing external Ca2+ with Mg2+. 7. Various K+ channel blockers including tetraethylammonium chloride (30 mM), 4-aminopyridine (4 mM) and apamin (100 nM) did not abolish I5-HT. Application of extracellular Cs+ (10 mM) blocked I5-HT-8. Peak inward I5-HT became larger with increasing extracellular K+. With low Clpipette solution (< 1 mM), or in low extracellular Na+ solution (26 mM), the inward I5-HT was not abolished.9. The current-voltage relation of I5-HT displayed inward rectification. In high external K+ concentration (20 mM), the reversal potential was about -29 mV, which is close to that of the inward rectifier evoked in motoneurones by membrane hyperpolarization.

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Substance P and 5-hydroxytryptamine immunoreactive presynaptic boutons on presumed .ALPHA.-motoneurons in the chicken ventral horn.

Cited by 31 publications

References 34 publications

Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Direct excitation of rat spinal motoneurones by serotonin.

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Substance P and 5-hydroxytryptamine immunoreactive presynaptic boutons on presumed .ALPHA.-motoneurons in the chicken ventral horn.

Cited by 31 publications

References 34 publications

Involvement of 5‐HT2 receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Involvement of 5‐HT2 receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Direct excitation of rat spinal motoneurones by serotonin.

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Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats

Involvement of 5‐HT₂ receptors in the behaviours produced by intrathecal administration of selected 5‐HT agonists and the TRH analogue (CG 3509) to rats