Substance p-containing primary sensory neurons projecting to the inferior mesenteric ganglion: Evidence from combined retrograde tracing and immunohistochemistry

Dalsgaard, C.‐J.; ̈kfelt, T. Ho; Elfvin, L.-G.; Skirboll, L.R.; Emson, P.C.

doi:10.1016/0306-4522(82)90070-7

Cited by 146 publications

(37 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently a slow excitatory potential has been described in neurones of the inferior mesenteric ganglia (Neild, 1978); its mediator has been tentatively identified as substance P or substance P-like peptide which may be released from collateral branches of sensory fibres arising from the gastrointestinal tract, thus providing a functional connexion between sensory and autonomic neurones (Dalsgaard, H6kfelt, Elfvin, Skirboll & Emson, 1982;Jiang, Dun & Karczmar, 1982;Matthews & Cuello, 1982;Tsunoo, Konishi & Otsuka, 1982;Dun & Kiraly, 1983). Synaptic transmission in neurones of the guinea-pig coeliac superior mesenteric plexus, on the other hand, has been studied only with respect to the fast excitatory post-synaptic potential (e.p.s.p.…”

mentioning

confidence: 99%

Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Dun¹,

C²

1984

The Journal of Physiology

View full text Add to dashboard Cite

mentioning

confidence: 99%

Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Dun¹,

C²

1984

The Journal of Physiology

View full text Add to dashboard Cite

“…Because the distension-induced depolarizations are transmitted to the IMG via the lumbar colonic nerves, the hypogastric nerves were stimulated in these preparations to preserve the integrity of the IMG with the colon and to avoid altering colonic contractility. Previous studies have shown that both the lumbar colonic nerves and the hypogastric nerves contain SP of central origin (19,20). Each of these depolarizations is also associated with an increase in membrane resistance (not shown).…”

Section: Resultsmentioning

confidence: 90%

“…Furthermore, there is no cross-desensitization between SP and vasopressin (6), another putative peptide neurotransmitter in sympathetic ganglia, and not all slow EPSPs are attenuated by substance P desensitization. SP in the IMG is localized in collateral terminals of primary sensory neurons of the C-fiber type, which originate in the dorsal root ganglia (19,20,29 In addition to those recorded in guinea pig prevertebral ganglia, fast and slow synaptic potentials have been recorded in other ganglia by using nerve-stimulation techniques. In both the rabbit superior cervical ganglion (33) and amphibian paravertebral sympathetic ganglion (34,35), preganglionic nerve stimulation elicits a fast EPSP, followed by a slow inhibitory potential (IPSP), a slow EPSP, and, finally, a late slow EPSP.…”

Section: Discussionmentioning

confidence: 99%

Fast and slow synaptic potentials produced in a mammalian sympathetic ganglion by colon distension.

Peters¹,

Kreulen²

1986

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Radial distension of the large intestine produced a slow depolarization in a population of neurons in the inferior mesenteric ganglion of the guinea pig. The slow potentials often occurred simultaneously with cholinergic fast potentials [(excitatory postsynaptic potentials (EPSPs)] yet persisted in the presence of nicotinic and muscarinic cholinergic antagonists when all fast EPSPs were absent. The amplitude of the distension-induced noncholinergic slow depolarization increased with increasing distension pressure. For distensions of 1-min duration at pressures of 10-20 cm of water, the mean depolarization amplitude was 3.4 mV. The slow depolarization was associated with an increase in membrane resistance, and prolonged periods ofcolon distension resulted in a tachyphylaxis of the depolarization. Desensitization of ganglion cells to the peptide substance P attenuated the distension-induced slow potential by an average of 49% ± 17%. Thus, two colonic mechanosensory afferent pathways converge on principal ganglion cells in the inferior mesenteric ganglion: one was previously described to be mediated by acetylcholine, and the other is described here, whose transmitter remains to be determined but which preliminary evidence suggests is mediated in part by substance P. The noncholinergic afferent pathway may enhance the intestinal inhibitory reflex mediated by cholinergic mechanosensory afferent input to the abdominal prevertebral sympathetic ganglia.In the prevertebral sympathetic ganglia, two general types of postsynaptic potential are recorded intracellularly. These potentials can be differentiated by their time course and the transmitters that mediate them. Fast excitatory postsynaptic potentials (EPSPs) can be evoked with single nerve shocks and are mediated by acetylcholine acting at nicotinic receptors. Slow EPSPs, elicited by repetitive nerve stimulation, are not mediated by acetylcholine (1); rather, other putative neurotransmitters have been implicated for the slow EPSP, including serotonin (2), substance P (SP) (3)(4)(5), and vasopressin (6, 7). Both fast and slow potentials can be evoked by stimulation of preganglionic or postganglionic nerves. The fibers activated by stimulation of the latter could be of two kinds: (i) branches of primary sensory neurons that traverse the ganglia (8, 9) and (ii) afferent neurons located in the gastrointestinal tract that project to the ganglia (8,(10)(11)(12). A cholinergic mechanosensory pathway to the prevertebral ganglia from the colon of the guinea pig has been described (13-17). Activation ofthis pathway by distension of the colon results in an increase in the frequency and amplitude of cholinergic EPSPs in ganglionic neurons. This pathway has been shown to mediate the afferent limb of a mechanosensitive intestinal reflex (16), in which distension of a segment of colon inhibits motility in an adjacent segment (10). It is not known whether the gastrointestinal mechanosensory pathway also comprises noncholinergic fibers; indeed, it remains to be determined whet...

show abstract

“…The coupling of these receptors to physiological responses involves G proteins, usually linked to the inhibition of one or more potassium conductances (Nakajima et al, 1988;Stanfield et al, 1985;Nowak & Macdonald, 1982). In sympathetic ganglia substance P is localized in ganglionic cell bodies and fibres of intrinsic interneurones (Hokfelt et al, 1977;Robinson et al, 1980;Dalsgaard et al, 1982). When released by either electrical or chemical stimulation this peptide elicits a postsynaptic depolarization caused partly by the inhibition of postsynaptic potassium conductances which includes, in some ganglia, the voltage-dependent M-current (Adams et al, 1983;Konishi et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Differences in neurokinin receptor pharmacology between rat and guinea‐pig superior cervical ganglia

Seabrook

Main

Bowery

et al. 1992

British J Pharmacology

View full text Add to dashboard Cite

1 The depolarizations elicited by seven neurokinin receptor agonists were examined in both rat and guinea-pig superior cervical ganglia by use of grease-gap methodology in the presence of tetrodotoxin (0.1 pM). Responses were normalised with respect to 1 AM eledoisin.2 The rank order of agonist potency in the rat ganglia was senktide > substance P > substance P methyl ester = eledoisin = Sar-Met-substance P > neurokinin B > neurokinin A, whereas in guinea-pig superior cervical ganglion (SCG) the rank order was senktide > Sar-Met-substance P > neurokinin B = eledoisin = substance P methyl ester. The concentration-effect curves for substance P and neurokinin A in guinea-pig ganglia were biphasic which precluded the determination of meaningful potency values. 3 The maximal depolarization achieved by subtype selective ligands was different between these two species. On rat and guinea-pig SCG, the NK3-selective ligand, senktide, produced a maximal depolarization of 27% and 274% respectively, whereas the NK,-selective ligand, substance P methyl ester, produced depolarizations of 77% and 64% respectively. 4 The depolarizations induced by substance P methyl ester and senktide in either species were unaffected by atropine (1 pM), suggesting a lack of involvement of presynaptic neurokinin receptors in the generation of the response. 5 The potency of substance P methyl ester, senktide, and neurokinin A were unaffected by pretreating ganglia with the peptidase inhibitors bacitracin (40pgmlm'), leupeptin (4fpgmhl'), and chymostatin (2 g ml-1). Similarly, these peptidase inhibitors had no effect on the maximal depolarizations achieved by any of these agonists. 6 It is evident that rat and guinea-pig superior cervical ganglia possess both NK, and NK3 receptors, but that their net contribution to depolarizations are different between the two species. The depolarizations in guinea-pig SCG are mediated predominantly by an NK3 subtype and in rat SCG by an NK, receptor subtype.

show abstract

Substance p-containing primary sensory neurons projecting to the inferior mesenteric ganglion: Evidence from combined retrograde tracing and immunohistochemistry

Cited by 146 publications

References 35 publications

Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Fast and slow synaptic potentials produced in a mammalian sympathetic ganglion by colon distension.

Differences in neurokinin receptor pharmacology between rat and guinea‐pig superior cervical ganglia

Contact Info

Product

Resources

About