Substance P receptor expression by inhibitory interneurons of the rat hippocampus: Enhanced detection using improved immunocytochemical methods for the preservation and colocalization of GABA and other neuronal markers

Sloviter, Robert S.; Ali-Akbarian, Leila; Horvath, Kathryn D.; Menkens, Kent A.

doi:10.1002/1096-9861(20010212)430:3<283::aid-cne1031>3.0.co;2-v

Cited by 85 publications

(39 citation statements)

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“…The pattern of distributions of the different markers in CA1 and CA3 presented in this study resembled that observed in previous studies (Kosaka et al, 1985; Sloviter and Nilaver, 1987; Sloviter, 1989; Freund and Buzsaki, 1996; Sloviter et al, 2001). The CA2 region contains interneurons that express, from the most abundant to the least abundant, Reelin, NPY, SOM, CR, CB, CCK, PV, and VIP (Figure 2) and their distribution in CA2 is layer-specific.…”

Section: Discussionsupporting

confidence: 91%

Distribution of interneurons in the CA2 region of the rat hippocampus

et al. 2014

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The CA2 region of the mammalian hippocampus is a unique region with its own distinctive properties, inputs and pathologies. Disruption of inhibitory circuits in this region appears to be linked with the pathology of specific psychiatric disorders, promoting interest in its local circuitry, its role in hippocampal function and its dysfunction in disease. In previous studies, CA2 interneurons, including a novel subclass of CA2 dendrite-preferring interneurons that has not been identified in other CA regions, have been shown to display physiological, synaptic and morphological properties unique to this sub-field and may therefore play a crucial role in the hippocampal circuitry. The distributions of immuno-labeled interneurons in dorsal CA2 were studied and compared with those of interneurons in CA1 and CA3. Like those in CA1 and CA3, the somata of CA2 parvalbumin-immunoperoxidase-labeled interneurons were located primarily in Stratum Pyramidale (SP) and Stratum Oriens (SO), with very few cells in Stratum Radiatum (SR) and none in Stratum Lacunosum Moleculare (SLM). There was, however, a greater proportion of GAD-positive cells were immunopositive for PV in SP in CA2 than in CA1 or CA3. CA2 SP also contained a larger density of somatostatin-, calbindin-, and VIP-immunopositive somata than CA1 and/or CA3. Like those in CA1 and CA3, CCK-immunopositive somata in CA2 were mostly located in SR. Reelin- and NPY- immunolabeled cell bodies were located in all layers of the three CA regions. However, a higher density of Reelin-positive somata was found in SP and SR of CA2 than in CA1 or CA3.

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Section: Discussionsupporting

confidence: 91%

Distribution of interneurons in the CA2 region of the rat hippocampus

et al. 2014

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“…While there are reports of reductions in other interneuronal markers, including somatostatin (Morris et al, 2008; Fung et al, 2010; Konradi et al, 2011) and cholecystokinin (Curley and Lewis, 2012), in schizophrenia patients, corresponding experiments in the MAM model have not been conducted. In addition, PV-expressing interneurons represent the largest population of interneurons in the HPC along with previously reported high co-localization with the SP-receptor (Sloviter et al, 2001). Reductions in PV have previously been reported in the MAM model in both the mPFC and ventral subiculum (Lodge et al, 2009).…”

Section: Discussionsupporting

confidence: 56%

“…Unlike the PFC where there is a low-level of co-expression of PV and SP-receptors (Matute et al, 1993), the HPC is especially amenable for the quantification of both proteins. Consequently, in the present study we assessed changes in PV and SP-receptor expression in HPC subregions known to exhibit a high-degree of co-expression of the two proteins, the dentate and both ventral and dorsal CA3 subfields (Leranth and Nitsch, 1994; Sloviter et al, 2001) of MAM (N=8) and SAL (N=8) rats (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

“…The co-expression of SP receptors and PV has previously been reported for the dentate, CA1, and CA3 subregions of the dorsal HPC (Acsady et al, 1997; Sloviter et al, 2001) and alterations in the co-expression has been used to quantify changes in HPC interneuron number in animal models of epilepsy (Sloviter, 1991; Sloviter et al, 2001; Sloviter et al, 2003), in that substance P receptors are continuously expressed even when activity-dependent PV content is lost. Verifying the extent of PV-expressing interneuron cell survival by measuring the co-expression of SP and PV in the HPC in the MAM model would be consistent with the pathological findings reported in schizophrenia patients.…”

Section: Introductionmentioning

confidence: 95%

“…The substance P (SP) receptor protein is expressed exclusively on nonprincipal, GABAergic interneurons in the hippocampus (Sloviter et al, 2001). The co-expression of SP receptors and PV has previously been reported for the dentate, CA1, and CA3 subregions of the dorsal HPC (Acsady et al, 1997; Sloviter et al, 2001) and alterations in the co-expression has been used to quantify changes in HPC interneuron number in animal models of epilepsy (Sloviter, 1991; Sloviter et al, 2001; Sloviter et al, 2003), in that substance P receptors are continuously expressed even when activity-dependent PV content is lost.…”

Section: Introductionmentioning

confidence: 99%

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Corresponding decrease in neuronal markers signals progressive parvalbumin neuron loss in MAM schizophrenia model

Gill

Grace

2014

Int. J. Neuropsychopharm.

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Alteration in normal hippocampal (HPC) function attributed to reduced parvalbumin (PV) expression has been consistently reported in schizophrenia patients and in animal models of schizophrenia. However, it is unclear whether there is an overall loss of interneurons as opposed to a reduction in activity-dependent PV content. Co-expression of PV and the constitutively-expressed substance P (SP)-receptor protein has been utilized in other models to ascertain the degree of cell survival, as opposed to reduction in activity-dependent PV content, in the HPC. The present study measured the co-expression of PV and SP-receptors in the dentate and dorsal and ventral CA3 subregions of the HPC in the methylazoymethanol acetate (MAM) rat neurodevelopmental model of schizophrenia. In addition, these changes were compared at the postnatal day 27 (PND27) and post-natal day 240 (PND240) time points. Brains from PND27 and PND240 MAM (n=8) and saline (SAL, n=8) treated offspring were immunohistochemically processed for the co-expression of PV and SP-receptors. The dorsal dentate, dorsal CA3 and ventral CA3 subregions of PND27 and PND>240 MAM rats demonstrated significant reductions in PV but not SP-receptor expression, signifying a loss of PV-content. In contrast, in the ventral dentate the co-expression of PV and SP-receptors was significantly reduced only in PND>240 MAM animals, suggesting a reduction in cell number. While MAM-induced reduction of PV content occurs in CA3 of dorsal and ventral HPC, the most substantial loss of interneuron number is localized to the ventral dentate of PND>240 animals. The disparate loss of PV in HPC subregions likely impacts intra-HPC network activity in MAM rats.

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Correlation between extracellular glucose and seizure susceptibility in adult rats

Schwechter

Velı́šková

Velı́šek

2002

Annals of Neurology

107

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In adult diabetic patients, periods of hyperglycemia may be associated with exacerbation of focal seizures. Our objective was to determine in the adult rats the correlation between seizure susceptibility and extracellular glucose concentration in two models of seizures. Male rats were injected with two doses of streptozocin (40 mg/kg IP) on 2 consecutive days to induce diabetic hyperglycemia. Controls either received vehicle or were not injected. After 2 weeks, blood glucose concentration was measured, and the rats were subjected to flurothyl seizure test. Another group of rats received glucose solution (20%, 5 ml IP) 30 minutes before testing to induce nondiabetic hyperglycemia. Thresholds for flurothyl-induced clonic and tonic-clonic seizures were determined. Finally, in vitro epileptiform activity was induced in the entorhinal cortex-hippocampal slices from naive rats by perfusing with magnesium-free medium with various glucose concentrations. In additional slices, paired-pulse paradigm was determined in the perforant path. Susceptibility to clonic and tonic-clonic flurothyl-induced seizures positively correlated with blood glucose concentrations as the increased glucose concentration was associated with proconvulsant effects. Similarly, in the in vitro experiments, epileptiform activity was promoted by increased and suppressed by decreased glucose concentrations. Data indicate that, in the adult rats, high glucose concentrations are associated with proconvulsant effects.

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Substance P receptor expression by inhibitory interneurons of the rat hippocampus: Enhanced detection using improved immunocytochemical methods for the preservation and colocalization of GABA and other neuronal markers

Cited by 85 publications

References 40 publications

Distribution of interneurons in the CA2 region of the rat hippocampus

Distribution of interneurons in the CA2 region of the rat hippocampus

Corresponding decrease in neuronal markers signals progressive parvalbumin neuron loss in MAM schizophrenia model

Correlation between extracellular glucose and seizure susceptibility in adult rats

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