Substitute for Benzidine in Myeloperoxidase Stains

Kaplow, Leonard S.

doi:10.1093/ajcp/63.3.451

Cited by 175 publications

(63 citation statements)

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“…Biotinylated horse anti-mouse Ig antibodies, heavy and light chain-specific (Vector), were used to detect B cells/plasma cells and immunoglobulin deposits. Binding of biotin-labeled primary or secondary antibodies was detected by stepwise incubation with avidinbiotin-peroxidase complexes (19) and 3-amino-9-ethylcarbazole containing H 2 0 2 (20). All sections were counterstained with Mayer's hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical characterization of synovial cells in arthritic mrl‐lpr/lpr mice

Tarkowski

Jönsson

Holmdahl

et al. 1987

Arthritis & Rheumatism

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical characterization of synovial cells in arthritic mrl‐lpr/lpr mice

Tarkowski

Jönsson

Holmdahl

et al. 1987

Arthritis & Rheumatism

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“…The staining specificity was checked on sections of the normal rat spleens. The peroxidase activity was then visualized according to Kaplow [12].…”

Section: Preparation Of Splenic Mononuclear Cells (Mnc)mentioning

confidence: 99%

Interferon‐γ and Interleukin‐12 Genes are Preferentially Expressed During Early Experimental African Trypanosomiasis and Suppressed by Denervation of the Spleen

Liu

Ragaa

et al. 1999

Scand J Immunol

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The cross talk between the central nervous system (CNS) and the immune system includes among others, the modulation of immune responses by the autonomic nervous system. Here, we investigated the effects of a splenic denervation on cytokine induction in early experimental African trypanosomiasis. Profiles of the cytokine mRNA expression for interleukin (IL)‐4, interleukin (IL)‐6, interleukin (IL)‐10, interleukin (IL)‐12, tumour necrosis factor (TNF)‐α, tumour necrosis factor (TNF)‐β, transforming growth factor (TGF)‐β and interferon (IFN)‐γ were examined at 4 h, 8 h and 12 h postinfection (p.i.), and in noninfected controls. Only IFN‐γ and IL‐12 were significantly expressed over noninfected controls. Already at 4 h p.i. both cytokines were expressed and showed more increased levels at 12 h. Sympathetic denervation of the spleen markedly reduced the mRNA expression for both IFN‐γ and IL‐12. Con A was used as a positive control and showed an enhanced mRNA expression, which was also suppressed by a splenic denervation. To demonstrate that the mRNA expression had resulted in a cytokine production, we looked for the protein level of IFN‐γ at 4 h p.i. by immunohistochemistry and found increased levels of IFN‐γ, which was also inhibited by the denervation. Sham‐operated animals exhibited similar responses as the nondenervated controls. Our data present for the first time very early kinetics for a cytokine gene expression during an experimental African trypanosomiasis. Furthermore, the data suggest a regulatory role for the autonomic nervous system on cytokine responses at both the mRNA and the protein levels.

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“…fragments of sheep anti-rabbit IgG and rabbit anti-mouse IgG, respectively. After 2 additional washes, cells were centrifuged in a cytocentrifuge and the peroxidase reaction carried out as described through the use of 3-amino-9-ethyl carbazole (Sigma, St. Louis, MO) (21).…”

Section: Sources Of Tissuementioning

confidence: 99%

Immune functions of human synovial cells. phenotypic and t cell regulatory properties of macrophage‐like cells that express hla–dr

Klareskog

Forsum

Kabelitz

et al. 1982

Arthritis & Rheumatism

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Substitute for Benzidine in Myeloperoxidase Stains

Cited by 175 publications

References 0 publications

Immunohistochemical characterization of synovial cells in arthritic mrl‐lpr/lpr mice

Immunohistochemical characterization of synovial cells in arthritic mrl‐lpr/lpr mice

Interferon‐γ and Interleukin‐12 Genes are Preferentially Expressed During Early Experimental African Trypanosomiasis and Suppressed by Denervation of the Spleen

Immune functions of human synovial cells. phenotypic and t cell regulatory properties of macrophage‐like cells that express hla–dr

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