SUBSTRATE SPECIFICITY AND INHIBITION STUDIES ON AFRICAN CATFISH (<i>Clarias gariepinus</i>) LIVER GLUTATHIONE S-TRANSFERASE

Tithonia diversifolia, Cyperus rotundus and Hyptis suaveloensis ethanol extracts combinatorially and competitively inhibit affinity purified cowpea storage bruchid (Callosobrochus maculatus) glutathione S-transferase

“…The whole organism was homogenized in a buffer (10 mM Tris-HCl, 250 mM sucrose, 1 mM phenylmethanesulfonyl fluoride, 0.1 mM b-mecrapoethanol, pH 7.4) and centrifuged at 30,000g for 40 min to remove cell debris using Eppendorf cold centrifuge 5810 R. The supernatant was rapidly purified on GSH-Sepharose 4B column as earlier described by Kolawole and Ajele (2004). The GST was ultra filtrated with 5,000 M.W.…”