Substrate versatility of polyhydroxyalkanoate producing glycerol grown bacterial enrichment culture

Moralejo-Gárate, Helena; Kleerebezem, Robbert; Mosquera-Corral, Anuska; Campos, José Luis; Palmeiro-Sánchez, Tania; Loosdrecht, M.C.M. van

doi:10.1016/j.watres.2014.07.044

Cited by 30 publications

(15 citation statements)

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“…The previously produced VFA are used under dynamic feeding strategies (e.g., by imposing feast/famine and presence/absence of electron donor) to enrich the seed sludge with microbes that possess a high PHA storing capacity. Finally, the third step consists in the PHA accumulation in batch systems, where the biopolymer content of the previously enriched community is maximized (Albuquerque et al, 2013; Moralejo-Garate et al, 2014). …”

Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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Limits in resource availability are driving a change in current societal production systems, changing the focus from residues treatment, such as wastewater treatment, toward resource recovery. Biotechnological processes offer an economic and versatile way to concentrate and transform resources from waste/wastewater into valuable products, which is a prerequisite for the technological development of a cradle-to-cradle bio-based economy. This review identifies emerging technologies that enable resource recovery across the wastewater treatment cycle. As such, bioenergy in the form of biohydrogen (by photo and dark fermentation processes) and biogas (during anaerobic digestion processes) have been classic targets, whereby, direct transformation of lipidic biomass into biodiesel also gained attention. This concept is similar to previous biofuel concepts, but more sustainable, as third generation biofuels and other resources can be produced from waste biomass. The production of high value biopolymers (e.g., for bioplastics manufacturing) from organic acids, hydrogen, and methane is another option for carbon recovery. The recovery of carbon and nutrients can be achieved by organic fertilizer production, or single cell protein generation (depending on the source) which may be utilized as feed, feed additives, next generation fertilizers, or even as probiotics. Additionlly, chemical oxidation-reduction and bioelectrochemical systems can recover inorganics or synthesize organic products beyond the natural microbial metabolism. Anticipating the next generation of wastewater treatment plants driven by biological recovery technologies, this review is focused on the generation and re-synthesis of energetic resources and key resources to be recycled as raw materials in a cradle-to-cradle economy concept.

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Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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“…The results clearly suggest that the sludge enriched at the lowest feast/famine ratio (0.04) had the maximum capacity to accumulate PHA up to 21.8 wt%. Nonetheless, the maximum PHA content achieved in this study is still low compared to that achieved in SBRs with glycerol as a substrate (37.2–80 wt%) and in two‐stage CSTR AS systems fed with fermented molasses (61 wt%) or fermented paper mill wastewater (48 wt%) containing mixed volatile fatty acids, which are favored substrates for PHA production.…”

Section: Resultsmentioning

confidence: 58%

“…The PHA compositions obtained in the fed‐batch reactors using the sludge from the systems operated at feast/famine ratios of 0.04, 0.15, and 0.33 were 77:23, 98:2, and 100:0 as % HB:HV by mole, respectively. The obtained PHA was dominated by HB, which is a common monomer of PHA when glycerol is used as a carbon source . Nevertheless, HV might be produced through other substrates formed during glycerol degradation, which could be possible in mixed microbial cultures.…”

Section: Resultsmentioning

confidence: 99%

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Polyhydroxyalkanoate production using two‐stage continuous stirred tank activated sludge systems with glycerol as a carbon source

Heepkaew

Suwannasilp

2020

J of Chemical Tech & Biotech

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BACKGROUND Polyhydroxyalkanoate (PHA) is a biopolymer that can be used as a biodegradable plastic. The incorporation of PHA production into wastewater treatment processes is a promising strategy for resource recovery. This study investigated PHA production in two‐stage continuous stirred tank reactor (CSTR)‐activated sludge (AS) systems fed with synthetic wastewater containing glycerol. The effects of the hydraulic retention time (HRT) ratios of the first and the second stages, which reflects the feast/famine ratio, at 0.04, 0.15, and 0.33 were examined. RESULTS The PHA accumulation and PHA consumption patterns under feast/famine conditions were successfully achieved in systems operated at HRT ratios of 0.04, 0.15, and 0.33 with influent glycerol concentrations of 3000 mg COD/L. Then, the sludge from these systems was tested for PHA accumulation in fed‐batch reactors. Maximum PHA contents of 21.8 wt%, 13.1 wt%, and 8.7 wt% were obtained for the sludge from the systems operated at HRT ratios of 0.04, 0.15, and 0.33, respectively. From the microbial community analysis via 16S rRNA gene amplicon sequencing (MiSeq, Illumina), the relative abundance of betaproteobacteria decreased, whereas the relative abundances of alphaproteobacteria and gammaproteobacteria increased after cultivation in the two‐stage CSTR AS systems. CONCLUSION The highest PHA accumulation was achieved at the lowest HRT ratio of the first and the second stages in the two‐stage CSTR AS systems. This study serves as an example of integrating PHA production into wastewater treatment systems, which enables us to apply the concept of resource recovery from wastewater. © 2019 Society of Chemical Industry

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“…These results seemed to indicate that glycerol was the main carbon source for PG accumulation. Moralejo‐Gárate et al . reported a yield of 0.40 Cmmol PG /Cmmol gly using synthetic glycerol, slightly higher than the value obtained in the present study (0.33 Cmmol PG /Cmmol gly ).…”

Section: Resultsmentioning

confidence: 99%

Feasible microbial accumulation of triacylglycerides from crude glycerol

Fra-Vázquez

Pedrouso

Palmeiro-Sánchez

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Crude glycerol, a by‐product of the biodiesel production industry, was used to produce intracellular storage polymers for waste valorization. The enrichment of a mixed microbial culture (MMC) in microorganisms with the ability to accumulate intracellular polymers was performed in a sequencing batch reactor (SBR) submitted to feast–famine conditions. The effect of different carbon sources in the accumulation of biopolymers was investigated. RESULTS A MMC enriched in yeast and bacteria was obtained using crude glycerol as feedstock. Accumulation experiments performed with crude glycerol, synthetic glycerol and synthetic methanol showed the feasibility of the MMC to produce different biopolymers. Triacylglyceride (TAG) accumulation up to 46 wt% in yeast cells was promoted by the presence of residual lipids in crude glycerol. However, bacteria from class Betaproteobacteria used glycerol mainly to accumulate 28 wt% of polyglucose (PG) and methanol as carbon source for cell growth. CONCLUSIONS As waste valorization, a possible advantage which comes out of the present study is the use of open, non‐sterile and non‐defined systems to produce TAGs. These TAGs can potentially re‐enter the biodiesel production process helping on the maximisation of the feedstock used in this process. © 2018 Society of Chemical Industry

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Substrate versatility of polyhydroxyalkanoate producing glycerol grown bacterial enrichment culture

Cited by 30 publications

References 32 publications

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Polyhydroxyalkanoate production using two‐stage continuous stirred tank activated sludge systems with glycerol as a carbon source

Feasible microbial accumulation of triacylglycerides from crude glycerol

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