Successful delivery derived from cryopreserved rare human spermatozoa with novel cryopiece

Abstract

Herein, we report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with cryopiece cryopreserved rare human spermatozoa from severe male factor infertility patients. We established a novel cryopiece system on the basis of previous studies. In this study, 126 spermatozoa from four patients with non-obstructiveazoospermia (NOA) or severe oligozoospermia were stored in cryopiece and then thawed on the day of the oocyte retrieval, 88 (83%) spermatozoa were recovered with a 47.5% (38 of 80) motile rate. Routine ICSI were performed in enrolled 30 MII oocytes from their spouse with their own spermatozoa, respectively. Twenty-two (73%) fertilization and 19 (86%) zygote cleavage were observed. Finally, a total of 11 embryos were achieved and each female patient received a transplantation of two fresh embryos. Four healthy babies were born at term. In conclusion, our novel cryopiece can be applied in assisted reproduction through ICSI with an effective motile spermatozoa recovery rate, high fertilization rate, and successful pregnancy result.

Cryopreservation of single-sperm: where are we today?

“…3f) has been commercialized in China. Using this method, Sun et al [40] from the Shanghai First People's Hospital reported the birth of the first single-sperm cryotube baby in China in 2016. They applied this technique clinically in four patients with severe oligozoospermia or NOA.…”

“…Following ICSI, fertilization rate was 73%, and 19 (86%) zygote cleavage events were observed. Four healthy babies were born at term [40].…”

“…(e) (Adapted from Peng et al[39]) A cryoleaf bound to a handle by cotton thread is placed to a protective casing containing cotton wool at the bottom. (f) (Adapted from Sun et al[40]) Droplets and polypropylene piece of cryopiece system. (g) (Adapted from Ma et al[41]) The closed slice frozen carrier comprised self-made non-toxic polypropylene flakes and conventional sperm cryovials.…”

Sperm dondurma: Güncel gelişmeler

“…Bu çalışmada ICSI ile fertilizasyon %73 olarak bildirilirken termde doğum 4 olguda bildirilmiştir. [70] Gebelik elde etmede tekrarlayan donma ve çözünmeler, akrozom reaksiyonu bozukluğu, globozoospermi, anne yaşı ve başlangıç sperm parametrelerinin son derece önemli olduğu bilinse de bu parametrelere sahip olan ya da olmayan olgularda bir kere gebelik elde edildikten sonra oluşan embriyo kalitesinin benzer olduğu bildirilmektedir. [5,71] Taze ya da donmuş sperm ile yapılan yardımcı üreme yöntemlerinde elde edilen gebeliklerin başarısı da üzerinde çalışılan konulardan biridir.…”

Modified stepwise mini-incision microdissection testicular sperm extraction: a useful technique for patients with a history of orchidopexy affected by non-obstructive azoospermia

“…At the completion of the procedure, the tunica albuginea and skin were closed with 5-0 suture (Ethicon, Puerto Rico, USA). If sperm were present, they were cryopreserved using either the LSL straw (Cryo BioSystem, Paris, France) (Liu et al, 2017) or the cryopiece system (Sun et al, 2017), according to the quantity and quality of the spermatozoa available for ICSI.…”