Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia

Endo, Yuji; Fujii, Yoshitaka; Kurotsuchi, Shozo; Motoyama, Hiroaki; Funahashi, Hiroaki

doi:10.1016/j.fertnstert.2012.07.1128

Cited by 37 publications

(48 citation statements)

References 16 publications

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“…The sperm ultra‐rapid cryopreservation was performed using Cryotop as previously described (Endo et al ., ,b). Briefly, a droplet of 0.5 μl freezing medium was placed on the polypropylene strip of a Cryotop (Kitazato, Tokyo, Japan) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Chen

Qian

et al. 2014

Andrologia

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Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermatozoa from 21 Normozoospermic patients were collected and vitrified without CPAs or with sucrose in small volume using Cryotop. The sperm recovery rate, motility, viability, chromatin damage and DNA fragmentation were assessed. No significant difference was observed in the sperm recovery rate and motility rate between the spermatozoa cryopreserved without CPAs and with sucrose. The post-thawed spermatozoa cryopreserved without CPAs had a higher viability and lower damage to sperm chromatin and DNA than those cryopreserved with sucrose. These results suggest that small numbers of human spermatozoa can be successfully vitrified without CPAs using Cryotop.

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Section: Methodsmentioning

confidence: 99%

“…Until recently, Endo et al . (, ,b) reported that a small number of human spermatozoa can be successful vitrified using Cryotop. This indicated that Cryotop may be also a suitable carrier to cryopreserve human spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Chen

Qian

et al. 2014

Andrologia

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“…Such changes in culture conditions have been shown to be involved in the lipid peroxidation of cell membranes, nuclear DNA fragmentation, and delay of embryo development [5][6][7][8]. Several groups have reported the cryopreservation of a small number of sperm using devices for embryo vitrification [9,10] by embedding them into an alginate gel [11] and by injecting them into an empty zona pellucida (ZP) [12][13][14]. Sperm injection into an empty ZP is preferable to other methods because of low reproducibility in the vitrification device and reduced survival in the alginate gel.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Tomita

Sakai

Khanmohammadi

et al. 2016

J Assist Reprod Genet

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Purpose We investigated whether enzymatically fabricated hyaluronan (HA) microcapsules were feasible for use in the cryopreservation of a small number of sperm. Methods HA microcapsules were fabricated using a system of water-immiscible fluid under laminar flow. Three sperm were injected into a hollow HA microcapsule using a micromanipulator. Capsules containing injected sperm were incubated in a freezing medium composed of sucrose as the cryoprotectant and then placed in a Cryotop® device and plunged into liquid nitrogen. After thawing, the capsule was degraded by hyaluronidase, and the recovery rate of sperm and their motility were investigated. Results The HA microcapsule measuring 200 μm in diameter and with a 30-μm thick membrane was handled using a conventional intracytoplasmic sperm injection (ICSI) system, and the procedure involved the injection of sperm into the capsule. The HA microcapsules containing sperm were cryopreserved in a Cryotop® device and decomposed by the addition of hyaluronidase. The recovery rate of sperm after cryopreservation and degradation of HA microcapsules was sufficient for use in clinical practice (90 %). Conclusions Hollow HA microcapsules can be used for the cryopreservation of a small number of sperm without producing adverse effects on sperm quality.

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“…This device is suitable to handle small cells and has become popular in assisted reproductive clinics. In addition, it was recently successful for sperm cryopreservation by the CryoTop (9). Although the CryoTop is superior for cryopreservation of few spermatozoa, the spermatozoa were loaded into a small freezing drop on the top of the sheet by a manipulator in air.…”

Section: Discussionmentioning

confidence: 99%

“…Some investigators have reported that spermatozoa have been cryopreserved in a very small drop on a tip of CryoLoop or CryoTop. These devices had been developed to freeze embryos (7)(8)(9). However, it is difficult to find all spermatozoa immediately after thawing using these methods because the spermatozoa are very small compared with embryos.…”

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confidence: 99%

Single human sperm cryopreservation method using hollow-core agarose capsules

Yao

Asayama

Matsuhisa

et al. 2015

Fertility and Sterility

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Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia

Cited by 37 publications

References 16 publications

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Single human sperm cryopreservation method using hollow-core agarose capsules

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