Successful Endothelialization of Porcine Glutaraldehyde-Fixed Aortic Valves in a Heterotopic Sheep Model

Gulbins, H; Pritisanac, A; Pieper, Korbinian; Goldemund, Angelika; Meiser, Bruno; Reichart, Bruno; Daebritz, Sabine

doi:10.1016/j.athoracsur.2005.11.011

Cited by 22 publications

(24 citation statements)

References 21 publications

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“…Due to the complexity of an aortic valve conduit implantation in large animals, all available studies to date, describe heterotrophic valve transplantations while some groups have implanted the TE aortic valve in the descending aorta [10,12]. These transplantations resulted in a non-physiological flow pattern and a permanently open valve, potentially promoting valvular degeneration [13].…”

Section: Discussionmentioning

confidence: 99%

Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model

et al. 2009

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“…As demonstrated in Figure 5, with the procedure used by Gulbins et al, 13,15 the concentration of free aldehyde groups ranged between 68% and 76% compared with the concentration of the untreated glutaraldehyde-fixed pericardium as a control with 100%. After partial detoxification, most of the extremely vulnerable endothelial cells did not survive ( Figure 6).…”

Section: Detoxification Processesmentioning

confidence: 99%

“…Such preseeded layers on porcine heart valves were stable in a sheep model. 14,15 Because of the fibroblasts, however, the valves showed a thickening of the leaflets that resulted in an impairment of function. In the present investigations, we sought to develop detoxification strategies and apply nanotechnological methods such as ultrathin titanium surface coating at temperatures between 30°C and 35°C 16 -18 on glutaraldehyde-fixed bovine pericardium as the base material for prosthetic heart valves.…”

Section: Clinical Perspective P 1660mentioning

confidence: 99%

“…The disadvantage of an additional fibroblast layer is that it induced a thickening and shrinking of these valves. 15 An initial nanotechnological procedure of plasma deposition with titanium using a high-frequency current for detoxification of glutaraldehyde-treated collagen dismissed most of the free aldehyde ligands. A reduced number of aldehyde groups should result in fewer chemical processes between free aldehyde groups and phospholipids, which are able to create calcification in combination with calcium ions from the circulation.…”

Section: Detoxification Processesmentioning

confidence: 99%

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Detoxification and Endothelialization of Glutaraldehyde-Fixed Bovine Pericardium With Titanium Coating

et al. 2009

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Background-Endothelial cell seeding of glutardialdehyde-fixed biological heart valves is hypothesized to improve biocompatibility and durability; however, the toxicity of glutardialdehyde prevents its use as a biological coating. Therefore, different detoxification strategies are applied, including surface coating with titanium, before in vitro endothelialization of glutaraldehyde-fixed bovine pericardium as the base material for prosthetic heart valves. Methods and Results-Bovine pericardium was fixed with 0.25% glutardialdehyde. Detoxification was performed with citric acid, aldehyde dehydrogenase, and plasma deposition with titanium at low temperatures of 30°C to 35°C. Toxic glutaraldehyde ligands were quantified photometrically, and the vitality of seeded cells was tested to validate detoxification methods. Detoxification agents and titanium coating were applied before seeding with human endothelial cells. Endothelial cells were visualized by electron microscopic surface scanning. To evaluate cell adhesion, shear stress was applied by a flow of 5 L/min over 24 hours. Compared with untreated glutaraldehyde-fixed samples, treatment with the different agents reduced free aldehyde groups gradually (citric acid 5% Ͻ citric acid 10% Ͻ titanium Ͻ aldehyde dehydrogenase). A combination of citric acid 10%, aldehyde dehydrogenase, and titanium coating resulted in a reduction of free aldehyde ligands to 17.3Ϯ4.6% (PՅ0.05) and demonstrated a vitality of seeded cells of 94Ϯ6.7% (PՅ0.05). This procedure yielded a completely confluent layer of regular human endothelial cells (nϭ5). After application of shear stress for 24 hours on these endothelial layers, cell vitality was 81%. Conclusions-Titanium coating combined with chemical procedures yielded significant detoxification and complete endothelialization of conventional glutaraldehyde-fixed pericardium. This new technique might improve glutardialdehyde-fixed cardiovascular bioimplants for better biocompatibility and longer durability.

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“…Há dificuldade de trabalhar com tais modelos, haja vista a dificuldade expressa na literatura devido ao número reduzido de animais utilizados nos vários trabalhos publicados (82)(83)(84)(85) .…”

Section: Discussionunclassified

Estudo experimental comparativo do enxerto homólogo pulmonar tratado pelo processo L-Hydro com o homoenxerto pulmonar a fresco

Paulo¹

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Successful Endothelialization of Porcine Glutaraldehyde-Fixed Aortic Valves in a Heterotopic Sheep Model

Cited by 22 publications

References 21 publications

Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model

Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model

Detoxification and Endothelialization of Glutaraldehyde-Fixed Bovine Pericardium With Titanium Coating

Estudo experimental comparativo do enxerto homólogo pulmonar tratado pelo processo L-Hydro com o homoenxerto pulmonar a fresco

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