Successful human in vitro fertilization using a modified human tubal fluid medium lacking glucose and phosphate ions

Quinn, Patrick; Moinipanah, Roya; Steinberg, Jeffrey M.; Weathersbee, Paul S.

doi:10.1016/s0015-0282(16)57504-9

Cited by 82 publications

(42 citation statements)

References 4 publications

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“…Their observations were a major influence on the design of medium CZB, which does not contain glucose and inorganic phosphate (24). Subsequently, a modification of Quinn's medium HTF for the culture of human embryos was formulated in which glucose and phosphate were omitted and EDTA and glutamine added (25). Additional changes resulted in medium P1 which is, in fact, a modified form of Quinn's glucose-free and phosphate-free modified HTF medium in which taurine and citrate are substituted for glutamine and EDTA, respectively (26).…”

Section: The Same Reviewers Comment Further Thatmentioning

confidence: 99%

Choosing a culture medium: making informed choices

Biggers¹,

Summers²

2008

Fertility and Sterility

134

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Section: The Same Reviewers Comment Further Thatmentioning

confidence: 99%

Choosing a culture medium: making informed choices

Biggers¹,

Summers²

2008

Fertility and Sterility

134

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“…These differences are of profound importance when handling gametes in vitro. For example, glucose will inhibit capacitation in bull sperm, but is required for capacitation in mouse sperm.The role of glucose in sperm metabolism of other species remains under investigation (Quinn et al 1995;Mahadevan et al 1997;Barak et al 1998;Williams and Ford 2001).…”

Section: Fuel Support and More: The Fibrous Sheathmentioning

confidence: 99%

Moving to the beat: a review of mammalian sperm motility regulation

Turner¹

2006

Reprod. Fertil. Dev.

248

184

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Abstract. Because it is generally accepted that a high percentage of poorly motile or immotile sperm will adversely affect male fertility, analysis of sperm motility is a central part of the evaluation of male fertility. In spite of its importance to fertility, poor sperm motility remains only a description of a pathology whose underlying cause is typically poorly understood. The present review is designed to bring the clinician up to date with the most current understanding of the mechanisms that regulate sperm motility and to raise questions about how aberrations in these mechanisms could be the underlying causes of this pathology.

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“…For mature oocyte collection, the female mice were further injected intraperitoneally with 5 IU of recombinant human chorionic gonadotropin (rec-hCG; Serono Canada) after 48 h of rec-FSH injection. Post 14 hours of rechCG injection, the mice were sacrificed and the oviducts were dissected and placed into a Petri-dish containing modified human tubal fluid-HEPES buffered medium (mHTF-HEPES) [Quinn et al 1995] supplemented with 1.0 mg/ml bovine serum albumin (BSA; Sigma, St. Louis, MO, USA). Cumulusoocyte complexes (COCs) were released by tearing the ampullae of the oviducts, COCs were denuded from cumulus cells by mechanically pipetting with a fine diameter pipette in mHTF-HEPES supplemented with 85 unit/ml hyaluronidase for assessment of maturity, and then mature oocytes were used for experiments.…”

Section: Mature and Immature Oocytes From Ovaries Stimulated By Gonadmentioning

confidence: 99%

“…Oocytes were transferred to 20 ml of mHTF-HEPES medium [Quinn et al 1995] supplemented with 1.0 mg/ml bovine serum albumin (BSA; Sigma) covered with warm paraffin oil in a Bioptechs dTC3 Culture Dish System (Bioptechs Inc., Butler, PA, USA). The system consists of a temperature controller, a stage adapter and a dTC3 Culture Dish, which has a specially coated clear glass bottom that is 0.15 mm thick.…”

Section: Analysis Of Spindle View With Polscopementioning

confidence: 99%

Cytogenetic Analysis ofIn VivoandIn VitroMatured Oocytes Derived from Naturally Cycling and Stimulated Mice

Wang

Zhou

et al. 2008

Systems Biology in Reproductive Medicine

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The objective of the study was to analyze the potential role of follicle stimulating hormone (FSH) in cytogenetic changes of in vivo and in vitro matured mouse oocytes and to determine whether the lower developmental potential of immature oocytes is due to a higher incidence of abnormalities in meiotic spindle organization and chromosome alignment as well as aneuploidy. In vivo matured oocytes were collected from naturally ovulated and superovulated (5.0 I U of recombinant follicle-stimulating hormone [rec-FSH] þ recombinant human chorionic gonadotropin [rec-HCG]) mice. Immature oocytes were retrieved from naturally cycling mice and from mice primed with rec-FSH for 48 h. The immature oocytes were cultured 18 h for in vitro maturation (IVM). In vivo and in vitro matured oocytes were assessed for the meiotic spindle organization and chromosome alignment as well as aneuploidy. There was no significant difference of meiotic spindle organization, chromosomal alignment and aneuploidy between in vivo and in vitro matured oocytes derived from naturally cycling and stimulated mice. Therefore, the lower developmental potential of immature oocytes does not appear to be directly related to the incidence of abnormal meiotic spindle organization and chromosome alignment or to aneuploidy.

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Successful human in vitro fertilization using a modified human tubal fluid medium lacking glucose and phosphate ions

Cited by 82 publications

References 4 publications

Choosing a culture medium: making informed choices

Choosing a culture medium: making informed choices

Moving to the beat: a review of mammalian sperm motility regulation

Cytogenetic Analysis ofIn VivoandIn VitroMatured Oocytes Derived from Naturally Cycling and Stimulated Mice

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