Successive ratio subtraction as a novel manipulation of ratio spectra for quantitative determination of a mixture of furosemide, spironolactone and canrenone

Smart Spectrophotometric Methods for Concurrent Determination of Furosemide and Spironolactone Mixture in Their Pharmaceutical Dosage Forms

“…Various analytical methods were established for the analysis of each of these of them, either alone or in combination with other drugs. Various methods were established for analysis of FUR, including polarographic and electrochemical methods (Semaan et al, 2008, Santini et al, 2009, Malode et al, 2012Kor, Zarei, 2016;Medeiros et al, 2016), spectrophotometric methods (Bosch et al, 2013;Sawant Ramesh et al, 2015;Emam et al, 2018) and chromatographic methods. These were to include also HPLC (Bosch et al, 2013)and HPTLC methods (Kher et al, 2013).…”

“…Various methods were established for analysis of SPR, including polarographic and electrochemical methods (Al-Ghamdi, Al-Ghamdi, Al-Omar, 2008;El-Shahawi et al, 2013;Smajdor, Piech, Paczosa-Bator, 2018), spectrophotometric methods (Dinç, Üstündağ, 2003;Tekerek, Şukuroglu, Okan, 2008;Hegazy et al, 2010;Golher, Kapse, Singh, 2011;, Kundu et al, 2017Emam et al, 2018) and various chromatographic methods. These included HPLC (Vaidya, Khanolkar, Gadre, 2002;Baranowska, Markowski, Baranowski, 2009;Ma et al, 2010;Vlase et al, 2011;Ram, Dave, Joshi, 2012;Walash et al, 2013;Woo et al, 2013;Lee et al, 2015;Rajalakshmi et al, 2018), HPTLC (Sharma et al, 2010;Kher et al, 2013).…”

Validated spectral manipulations for determination of an anti-neoplastic drug and its related impurities including its hazardous degradation product

“…Dual ratio subtraction (DRS) method depends on coupling of ratio subtraction and dual wavelength spectrophotometric methods, where the interference from the most extended component is eliminated using ratio subtraction method, then dual wavelength on the obtained spectra was applied by choosing two wavelengths where the component to be measured shows significant absorbance difference while the absorbance of the other interfering component is the same. First, ratio subtraction method steps were followed; 14 where division of the mixtures spectra ( X + Y ) by a certain concentration of X (the most extended component) was done, where new curves resulted ( Y / X ′ + [ X / X ′ = constant 1 ]). The constant values were then subtracted from the resulted ratio spectra, and the new obtained curves were multiplied by X ′ (the divisor).…”

“…Successive ratio subtraction method was recently developed for separation and quantitative determination of ternary mixtures. 14 For determination of the most un extended component [Z], the following steps were followed. First ratio subtraction method was applied as mentioned before in DRS, then the developed spectra were divided by Y ′, where the resulted curves represent ( X / Y ′ + [ Y / Y ′ = constant 2 ]).…”

Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma

“…For determination of SP and CN, different methods were reported using HPLC (Dong, Xu, Zhang, Tian, & Chen, ; Politi, Morini, & Polettini, 2014). The ternary mixture of SP, FR and CN was recently determined via a novel spectrophotometric method (Emam, Abdelaleem, Naguib, Abdallah, & Ali, ). The ternary mixture was also determined via HPLC MS/MS (Sora, Udrescu, Albu, David, & Medvedovici, ).…”