Succinate Dehydrogenase Subunit C Contributes to Mycelial Growth and Development, Stress Response, and Virulence in the Insect Parasitic Fungus Beauveria bassiana

Ding, Jin‐Li; Li, Xiuhui; Lei, Jiahui; Feng, Ming‐Guang; Ying, Sheng‐Hua

doi:10.1128/spectrum.02891-22

Cited by 21 publications

(16 citation statements)

References 38 publications

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“…Previous studies have indicated that SdhC was essential in maintaining SQR function and SdhC deletion mutants were either unsuccessful to obtained or resulted in mutants with a significant decrease in fitness. , To validate the function of FgSdhC and FgSdhC 1 , targeted deletions of two genes were performed in the isolates PH-1, F1405, and Fg131 (Figure S3). PH-1 and Fg131 were F.…”

Section: Resultsmentioning

confidence: 99%

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FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum

Sun,

Cai,

Liu

et al. 2023

J. Agric. Food Chem.

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Fusarium graminearum exhibited natural resistance to a majority of succinate dehydrogenase inhibitor fungicides (SDHIs) and the molecular mechanisms responsible for the natural resistance were still unknown. Succinate dehydrogenase subunit C (SdhC) is an essential gene for maintaining succinate-ubiquinone oxidoreductase (SQR) function in fungi. In F. graminearum, a paralog of FgSdhC named as FgSdhC 1 was identified. Based on RNA-Seq and qRT-PCR assay, we found that the expression level of FgSdhC 1 was very low but upregulated by SDHIs treatment. Based on reverse genetics, we demonstrated that FgSdhC 1 was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard F. graminearum isolate PH-1 had high sensitivity to a majority of SDHIs. A single nucleotide variation (C to T) in the FgSdhC 1 of isolate PH-1, resulting in a premature termination codon (TAA) replacing the fourth amino acid glutamine (Q), led to the failure of FgSdhC 1 to perform functions of conferring nature resistance. These results established that a dispensable paralogous gene determined SDHIs resistance in natural populations of F. graminearum.

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Section: Resultsmentioning

confidence: 99%

“…Similarly, in Beauveria bassiana, two SdhC species ( BbSdhC 1 and BbSdhC 2 ) were identified. BbSdhC 1 played a role in maintaining SQR function but that BbSdhC 2 was not sufficient …”

Section: Discussionmentioning

confidence: 99%

FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum

Sun,

Cai,

Liu

et al. 2023

J. Agric. Food Chem.

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“…41 Loss of the BbSdhC 1 in Beauveria bassiana leads to heightened intracellular ROS levels and impaired mycelial growth under oxidative stress conditions. 42 Mutations in SdhC of embryonic fibroblasts have been shown to increase ROS production and enhance tumorigenesis in human cells. 43,44 SDH also plays a significant role in plants regarding ROS production, regulation of plant development, and stress responses.…”

Section: ■ Discussionmentioning

confidence: 99%

Functional Differentiation of the Succinate Dehydrogenase Subunit SdhC Governs the Sensitivity to SDHI Fungicides, ROS Homeostasis, and Pathogenicity in Fusarium asiaticum

Chen,

Li,

Wei

et al. 2024

J. Agric. Food Chem.

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Succinate dehydrogenase (SDH) is an integral component of the tricarboxylic acid cycle (TCA) and respiratory electron transport chain (ETC), targeted by succinate dehydrogenase inhibitors (SDHIs). Fusarium asiaticum is a prominent phytopathogen causing Fusarium head blight (FHB) on wheat. Here, we characterized the functions of the FaSdhA, FaSdhB, FaSdhC 1 , FaSdhC 2 , and FaSdhD subunits. Deletion of FaSdhA, FaSdhB, or FaSdhD resulted in significant growth defects in F. asiaticum. The FaSdhC 1 or FaSdhC 2 deletion mutants exhibited substantial reductions in fungal growth, conidiation, virulence, and reactive oxygen species (ROS). The FaSdhC 1 expression was significantly induced by pydiflumetofen (PYD). The ΔFaSdhC 1 mutant displayed hypersensitivity to SDHIs, whereas the ΔFaSdhC 2 mutant exhibited resistance against most SDHIs. The transmembrane domains of FaSdhC 1 are essential for regulating mycelial growth, virulence, and sensitivity to SDHIs. These findings provided valuable insights into how the two SdhC paralogues regulated the functional integrity of SDH, ROS homeostasis, and the sensitivity to SDHIs in phytopathogenic fungi.

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“…The amplified fragments and the linearized pRS426 plasmid were cotransformed into S. cerevisiae (FY834) to obtain the correct knockout vector pRS426-AoSte12-hph, and the disruption sequences were amplified from it using Ste12LF1 and Ste12RR1 and then transformed into A. oligospora protoplasts, as previously described ( 67 , 68 ). The positive transformants were confirmed via PCR and Southern blotting, as described previously ( 45 , 69 ).…”

Section: Methodsmentioning

confidence: 99%

AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora

et al. 2023

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Nematode-trapping (NT) fungi are an important natural enemy of nematodes and can capture their prey by producing traps. Hyphal anastomosis and fusion are important for mycelial growth and the colony morphological development of filamentous fungi and are also crucial for the trap morphogenesis of NT fungi. Arthrobotrys oligospora can form complex three-dimensional networks (traps) when sensing the presence of nematodes.

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Succinate Dehydrogenase Subunit C Contributes to Mycelial Growth and Development, Stress Response, and Virulence in the Insect Parasitic Fungus Beauveria bassiana

Cited by 21 publications

References 38 publications

FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum

FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum

Functional Differentiation of the Succinate Dehydrogenase Subunit SdhC Governs the Sensitivity to SDHI Fungicides, ROS Homeostasis, and Pathogenicity in Fusarium asiaticum

AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora

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