Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater

Yang, Huameng; He, Peipei; Yin, Youcheng; Mao, Zhili; Zhang, Jing; Zhong, Chongquan; Xie, Tian; Wang, Anming

doi:10.1007/s00449-021-02583-x

Cited by 15 publications

(8 citation statements)

References 71 publications

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“…Figure C shows the thermogravimetric analysis (TGA) data for Cu 3 (PO 4 ) 2 and FBS-Cu 3 (PO 4 ) 2 . The initial weight loss of Cu 3 (PO 4 ) 2 and FBS-Cu 3 (PO 4 ) 2 below 150 °C is associated with the loss of physically adsorbed water. , FBS-Cu 3 (PO 4 ) 2 showed a weight loss at 150–350 °C, corresponding to the loss of low-molecular-weight compounds . Above 350 °C, higher weight loss denoting the degradation of proteins originating from FBS and structurally bound water molecules occurred, , suggesting the presence of protein in FBS-Cu 3 (PO 4 ) 2 .…”

Section: Resultsmentioning

confidence: 97%

“… 12 , 20 FBS-Cu 3 (PO 4 ) 2 showed a weight loss at 150–350 °C, corresponding to the loss of low-molecular-weight compounds. 21 Above 350 °C, higher weight loss denoting the degradation of proteins originating from FBS and structurally bound water molecules occurred, 12 , 20 suggesting the presence of protein in FBS-Cu 3 (PO 4 ) 2 .…”

Section: Resultsmentioning

confidence: 99%

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Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Mochizuki

Nakamura

2022

ACS Omega

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Fetal bovine serum (FBS) particles, which mainly consist of bovine serum albumin, have the potential for biological and medical applications as drug carriers. The coacervation of albumin is a common technique for preparing albumin-based particles. The replacement of salt with novel metal salts such as Cu is an affordable way to embed the metal ion in the albumin-based particles. Further, increased Cu distribution is prevalent in many cancers. Here, we prepared adhesive cell-like FBS–copper phosphate hybrid particles [FBS-Cu 3 (PO 4 ) 2 ], which exhibited toxicity toward cancer cells, with a narrow size distribution under cell culture conditions for preventing tumor progression. FBS-Cu 3 (PO 4 ) 2 showed peroxidase-like activity. In addition, FBS-Cu 3 (PO 4 ) 2 was successfully loaded with rhodamine B and conjugated with fluorescein isothiocyanate as models of drugs by coincubation. Thus, we designed a simple preparation method for optimizing FBS-Cu 3 (PO 4 ) 2 synthesis under cell culture conditions. FBS-Cu 3 (PO 4 ) 2 has significant potential as an efficient reactive oxygen species generator and drug-delivery agent against cancer cells. Furthermore, the RhoB-loaded FBS-Cu 3 (PO 4 ) 2 successfully interacted with 4T1 mouse mammary tumor cells and were confirmed to exhibit toxicity.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Mochizuki

Nakamura

2022

ACS Omega

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“…According to Somturk et al ., the optimal CuSO 4 concentration for synthesizing urease hybrid nanoflowers was 0.8 mM 32 . During the synthesis of nanoflowers, coordination interactions between the functional moieties of the enzyme and metal ions strengthens structural rigidity, thus reducing the extent of conformational shift and avoiding enzyme denaturation in harsh conditions 36,37 …”

Section: Resultsmentioning

confidence: 99%

“…43 Furthermore, the laccase-Cu 3 (PO 4 ) 2 nanoflowers retained 85% of their activity during 10 cycles of reuse. 36 The decline in CA activity after multiple reuse cycles could be due to the enzyme's leaching during separation.…”

Section: Kinetic Analysismentioning

confidence: 99%

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Sharma

Nadda

2023

J of Chemical Tech & Biotech

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BACKGROUND: Carbonic anhydrase (CA) is one of the most widely distributed enzymes among plants, animals, and microorganisms, and it has an enormous ability to capture carbon dioxide (CO 2 ). However, the real-time application of CA is still a challenge due to its low operational stability, its difficulty in recovery from the reaction medium, and its poor durability.RESULTS: The synthesis of insoluble protein inorganic hybrid structures at nanoscale was proven as quite useful to catalyze enzymatic biotransformation. Here, CA nanoflowers (CANF) were synthesized with the self-assembly of metal phosphate and CA. The synthesis of CANF was performed using 0.2 mg mL −1 protein and 2.0 mM CuSO 4 at 4 °C under mild shaking conditions. The CANF exhibited optimum activity at pH 7.5 and a temperature of 40°C. The synthesized CANF were used for CO 2 conversion under optimized conditions and their kinetic parameters were studied using p-NPA hydrolysis. The V max and K m of CANF were 185.18 ∼mol min −1 mL −1 and 4.72 mM, compared with those of free CA, 166.66 ∼mol min −1 mL −1 and 5.12 mM, respectively. The stability of CANF has improved remarkably. The CANF made of metal ions and protein showed higher stability and enzyme activity than free enzymes. Furthermore, the CANF showed good reusability due to their mechanical properties and monodispersity. The production of CaCO 3 by CANF was 1.71-fold higher than that by free CA. CONCLUSION:The newly formed CANF showed flower-like morphology with good catalytic activity. This study demonstrated that CANF technology has a bright future in the conversion of CO 2 into CaCO 3 .

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“…12 The enzyme activity is the key to the sensitivity of colorimetric detection. 13 Natural enzymes including horseradish peroxidase 14 and laccase 15 have become a the choice for colorimetric detection because of their excellent activities. However, natural enzymes are easy to inactivate and costly.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of dual function Fe₃O₄@MnO₂@HKUST-1 magnetic micromotors for efficient colorimetric detection and degradation of hydroquinone

Luo

Han

et al. 2023

New J. Chem.

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Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater

Cited by 15 publications

References 71 publications

Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Synthesis of dual function Fe₃O₄@MnO₂@HKUST-1 magnetic micromotors for efficient colorimetric detection and degradation of hydroquinone

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Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater

Cited by 15 publications

References 71 publications

Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Preparation of Fetal Bovine Serum–Copper Phosphate Hybrid Particles under Cell Culture Conditions for Cancer Cell Treatment

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO2 into value added product

Synthesis of dual function Fe3O4@MnO2@HKUST-1 magnetic micromotors for efficient colorimetric detection and degradation of hydroquinone

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Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Synthesis of dual function Fe₃O₄@MnO₂@HKUST-1 magnetic micromotors for efficient colorimetric detection and degradation of hydroquinone