Succinyl-β-cyclodextrin modified gold biochip improved seroimmunological detection sensitivity for Lyme disease

Ye, Lei; Huang, Na-Li; Du, Yang; Schneider, Marion; Du, Wei‐Dong

doi:10.1016/j.aca.2016.11.050

Cited by 3 publications

(6 citation statements)

References 34 publications

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“…Other CDs derivatives for more specific electrochemical applications are represented by succinyl-CDs [17], per-6-deoxy-per-6-(2-carboxy-methyl)thio-β-cyclodextrin (AcSCD) [18], lipoyl-CDs [19] or disulfides bridged two adjacent β-CDs (DB-β-CDs) [20].…”

Section: Chemical Modifications Of Cds For Electrochemical Devicesmentioning

confidence: 99%

Integration Methods of Cyclodextrins on Gold and Carbon Electrodes for Electrochemical Sensors

Casulli

Taurino

Carrara

et al. 2019

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Cyclodextrins (CDs) are oligosaccharides composed of six (α), seven (β) or eight (γ) glucose units. Their inner hydrophobic cavity and hydrophilic external surface enable the formation of the “host-guest inclusion complex” with different organic or inorganic molecules showing high molecular selectivity. For these characteristics, CDs have many potential applications in electrochemical sensing. To enable CDs immobilization on the electrode surfaces, different chemical modifications are needed depending of the electrode material, while nanomaterials have been exploited to enhance the sensing signal. The CDs binding onto gold nanoparticles or carbon nanotubes, as an electron-transfer mediator to the electrode surface, is a typical example of it, while also graphene is largely used. The aim of the present review is to give an overview of CDs properties and their applications to electrochemical sensors for medical diagnostics. Different kinds for the functionalization of CDs onto electrode surfaces will be reviewed as well as their performance in presence of nanomaterials. Finally, CDs-based devices for sensing biomedical molecules of biomedical interest will be briefly presented and discussed.

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Section: Chemical Modifications Of Cds For Electrochemical Devicesmentioning

confidence: 99%

Integration Methods of Cyclodextrins on Gold and Carbon Electrodes for Electrochemical Sensors

Casulli

Taurino

Carrara

et al. 2019

View full text Add to dashboard Cite

show abstract

“…More recently, gold-coated glass or silicon wafers are increasingly used for immobilising nucleic acids and proteins [125][126][127] . These surfaces are stable in laboratory conditions and can be readily functionalised by forming a self-assembled monolayer (SAM) of alkanethiolates using crosslinkers such as succinimidyl undecanoate, 16-amino-1-hexadecanethiol, or succinimidyl 3-(2-pyridyldithio)propionate (SPDP) [126][127][128][129] . Sometimes a hydrophobic (e.g.…”

Section: Protein Microarray Technology For Lyme Disease Diagnosismentioning

confidence: 99%

“…Various studies found that immobilised flagellar antigen and recombinant OspC peptides were able to detect IgG and IgM antibodies in LD patients with higher sensitivity than the two-tier algorithm 131,136 . Another microarray assay with a functionalised surface containing cyclic oligosaccharides was able to capture and immobilise a recombinant form of VlsE antigen via hydrophobic interactions 129 . Captured antigen binds noncovalently with the -OH groups of the cone structure, which was extended from the gold surface by 16-amino-1-hexadecanethiol to reduce steric hindrance effects of neighbouring probes and biomolecules 129 .…”

Section: Protein Microarray Technology For Lyme Disease Diagnosismentioning

confidence: 99%

“…Another microarray assay with a functionalised surface containing cyclic oligosaccharides was able to capture and immobilise a recombinant form of VlsE antigen via hydrophobic interactions 129 . Captured antigen binds noncovalently with the -OH groups of the cone structure, which was extended from the gold surface by 16-amino-1-hexadecanethiol to reduce steric hindrance effects of neighbouring probes and biomolecules 129 . Interestingly, the researchers found that the capture density was higher compared to covalently immobilised antigen 128,129 .…”

Section: Protein Microarray Technology For Lyme Disease Diagnosismentioning

confidence: 99%

“…Captured antigen binds noncovalently with the -OH groups of the cone structure, which was extended from the gold surface by 16-amino-1-hexadecanethiol to reduce steric hindrance effects of neighbouring probes and biomolecules 129 . Interestingly, the researchers found that the capture density was higher compared to covalently immobilised antigen 128,129 . The immobilised recombinant VlsE in this study was further used to detect serum antibodies in LD patients, with similar results as the VlsE ELISA.…”

Section: Protein Microarray Technology For Lyme Disease Diagnosismentioning

confidence: 99%

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