Sucrose is involved in the regulation of iron deficiency responses in rice (Oryza sativa L.)

Abstract

Sucrose signaling pathways were rapidly induced in response to early iron deficiency in rice plants, and the change of sucrose contents in plants was essential for the activation of iron deficiency responses. Sucrose is the main product of photosynthesis in plants, and it functions not only as an energy metabolite but also a signal molecule. However, a few studies have examined the involvement of sucrose in mediating iron deficiency responses in rice. In this study, we found that the decrease in photosynthesis and total chlorophyll concentration (SPAD values) in leaves occurred at a very early stage under iron deficiency. In addition, the sucrose was increased in leaves but decreased in roots of rice plants under iron deficiency, and also the sucrose transporter (SUT) encoded genes' expression levels in leaves were all inhibited, including OsSUT1, OsSUT2, OsSUT3, OsSUT4, and OsSUT5. The carbohydrate distribution was changed under iron deficiency and sucrose might be involved in the iron deficiency responses of rice plants. Furthermore, exogenous application of sucrose or dark treatment experiments were used to test the hypothesis; we found that the increased endogenous sucrose would cause the repression of iron acquisition-related genes in roots, while further stimulated iron transport-related genes in leaves. Compared to the exogenous application of sucrose, the dark treatment had the opposite effects. All the above results highlighted the important role of sucrose in regulating the responses of rice plants to iron deficiency.

Integration of embryo-endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly grains

“…A gene was declared as embryo or endosperm specific if it had a Z-score above 2 in at least 1 one of the embryo or endosperm samples as compared to all the same kind samples. 1 Chemical analysis for metabolites 1 Sucrose, fructose, glucose, and starch: Sugar contents were measured by ultra-high 1 performance liquid chromatography system (UHPLC) as described by Chen et al (2018a).…”

The Molecular Mechanisms Underlying Iron Deficiency Responses in Rice

“…Additionally, the exogenous application of sucrose and a reduction in endogenous sucrose concentrations by dark treatment led to opposite expression patterns for Fe-related genes in roots and leaves under Fe deficiency conditions. These results highlight that sucrose functions as a shoot-generated signaling substance mediating shoot-root communication in the responses of rice plants to Fe deficiency [94].…”

“…As the main product of plant photosynthesis, sucrose acts as an energy resource for growth and development, as well as a signaling substance that participates in the responses of plants to biotic and abiotic stresses, such as mineral starvation [94,95]. In rice, sucrose is found to take part in modulating Fe deficiency responses [94]. A greater amount of sucrose tends to accumulate in the leaves rather than in the roots of rice plants when grown under Fe-deficient conditions by suppressing the transcript abundance of SUT (sucrose transporter) genes in leaves.…”

Integrative Transcriptome and Metabolome Profiles Reveal Common and Unique Pathways Involved in Seed Initial Imbibition Under Artificial and Natural Salt Stresses During Germination of Halophyte Quinoa

“…It consists of glucose and fructose, which helps plants to answer abiotic stress ( Nemati et al, 2018 ). Sucrose synthase (SUS) and sucrose phosphate synthase (SPS) are two key enzymes involved in sucrose metabolism by catalyzing the production of UDP-glucose and sucrose-6-phosphate, respectively ( Chen et al, 2018 ). Alpha-glucosidase (AGLU), as a carbohydrate digestive enzyme, catalyzes the hydrolysis of alpha-glucopyranoside bonds in oligosaccharides and disaccharides to yield glucose.…”