Sucrose Phosphate Synthase and Sucrose Accumulation at Low Temperature

Abstract

The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 C for 3 weeks and then transferred to a constant 5 C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14-to 20-fold over the levels present at the outset of the low-temperature treatment. Sucrose was the most abundant free sugar before, during, and after exposure to 5°C. Leaf sucrose phosphate synthase activity was significantly increased by the low-temperature treatment, whereas sucrose synthase and invertases were not. Synthesis of the sucrose phosphate synthase subunit was increased during and after lowtemperature exposure and paralleled an increase in the steadystate level of the subunit. The increases in sucrose and its primary biosynthetic enzyme, sucrose phosphate synthase, are discussed in relation to adjustment of metabolism to low nonfreezing temperature and freezing stress tolerance.

In situ effects of elevated CO2 on the carbon and nitrogen status of alpine plants

“…In all species except Trifolium, the rise in leaf TNC found was primarily the result of an increase in sugars that, however, was moderate compared to the high sugar levels already observed under ambient CO 2 . Such high natural sugar concentrations in leaf tissue have often been found in cold-adapted plants (Mooney & Billings 1965;Pollock & Lloyd 1987) and are known to be correlated with frost resistance (Sakai & Larcher 1987;Guy, Huber & Huber 1992). The moderate rise in sugar concentration under elevated CO 2 may still increase cold hardiness.…”

Effects of drought on non‐mycorrhizal and mycorrhizal maize: changes in the pools of non‐structural carbohydrates, in the activities of invertase and trehalase, and in the pools of amino acids and imino acids

“…Proteins were extracted by grinding lyophilized material (1 g) in 5 ml of ice-cold 3-(N-morpholino) propane sulphonic acid (K + ) buffer (0n05  ; pH 7n5), containing 10 m MgCl # , 1 m EDTA, 5 m DTT, 0n1% (v\v) Triton-X2 100 (Boehringer, Mannheim, Germany) and 1 % (w\v) insoluble polyvinylpyrrolidone (Guy, Huber & Huber, 1992). The homogenate was centrifuged (13 000 g, 10 min) and the proteins in the resulting supernatant were precipitated with ammonium sulphate (70 % saturation) according to Mu$ ller, Boller & Wiemken (1996).…”

Changes in the sugar content of sweet sorghum stems under natural conditions during winter in saline soil of the Yellow River Delta

“…As the temperature decreases, the activity of SS and SPS is reduced, but it is estimated that it will maintain a high activity under natural conditions from the beginning of November 2016 to the end of February 2017. Previous studies have shown that the enzyme activities involving sucrose metabolism maintain a certain activity at low temperatures (Guy et al, 1992). of different varieties of sweet sorghum straw was not exactly the same (Lingle, 1987).…”