Suction‐modified Bergström muscle biopsy technique: Experience with 13,500 procedures

Abstract

The suction-modified Bergström needle muscle biopsy technique is safe and provides an adequate sample size for histologic, ultrastructural, DNA, and enzyme analysis.

Elevated SOCS3 and altered IL-6 signaling is associated with age-related human muscle stem cell dysfunction

“…A total of four percutaneous needle biopsies were obtained from the mid-portion of the vastus lateralis under local anesthetic (1% lidocaine) using manual suction (55). One muscle biopsy was obtained from the nonworking leg for baseline analysis (PRE).…”

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique

“…Twenty years later, Evans et al 5 modified the technique by applying suction through the cutting trocar of the Bergström needle. This modification can enhance the yield of tissue 3-to 5-fold 6,7 and is employed in clinical and biomedical research settings. This technique has and will continue to foster the diagnosis of myopathies and our understanding of the structure and function of skeletal muscle.…”

Generation and Use of Cultured Human Primary Myotubes

“…A number of techniques can be used to obtain a skeletal muscle biopsy including needle biopsy and surgical excision (Dietrichson et al, 1987;Tarnopolsky et al, 2011). Successful isolation of myogenic satellite cells can be undertaken from small samples of muscle biopsies ranging in size from 50-100 mg (wet weight) (McAinch et al, 2007).…”

“…Successful isolation of myogenic satellite cells can be undertaken from small samples of muscle biopsies ranging in size from 50-100 mg (wet weight) (McAinch et al, 2007). This suggests that the typical muscle yield from suction-enhanced needle biopsies is sufficient to culture viable primary myotubes (Melendez et al, 2007;Tarnopolsky et al, 2011). The use of needle biopsy has additional benefits in the relative ease of obtaining muscle biopsies, enabling sampling from a number of accessible skeletal muscles with only the use of local anaesthetic (Dietrichson et al, 1987).…”

“…The use of needle biopsy has additional benefits in the relative ease of obtaining muscle biopsies, enabling sampling from a number of accessible skeletal muscles with only the use of local anaesthetic (Dietrichson et al, 1987). The suction-enhanced method described by Tarnopolsky et al, (2011) is described briefly as follows.…”