Sugar and starch dynamics in the medium-root-leaf system indicate possibilities to optimize plant tissue culture

Lembrechts, Ruth; Ceusters, Nathalie; Proft, M.P. De; Ceusters, Johan

doi:10.1016/j.scienta.2017.06.015

Cited by 24 publications

(11 citation statements)

References 25 publications

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“…Previously, 3.4% sucrose in the acclimated plantlets of Rindera umbellata has been reported to promote survival rate in the greenhouse (71.43%) and field conditions (42.86%), leading to greater leaf length, fresh weight, dry weight, number of roots and root length than without hardening process (Perić et al 2012). Shoot dry weight of bromeliad Guzmania 'Hilda' plants grown in 4.5% sucrose exhibited positive relation to sucrose uptake (Lembrechts et al 2017). Moreover, survival percentage, number of shoots and vigorous growth with green plant of acclimatized pineapple plantlets under 3% table sugar and sucrose were validated as optimum dose when compared with glucose and fructose (Mengesha et al 2020).…”

Section: Discussionmentioning

confidence: 94%

“…Increased sugar level in root and leaf tissues of acclimatized plantlets depends on the types of sugar and a degree of exogenous application. For example, sucrose was rapidly taken up by root organs and then translocated to leaf tissues of acclimatized bromeliad 'Hilda' plantlets in relation to an initial sucrose concentration in the medium (15-117 µmol g −1 medium) within 4 weeks (Lembrechts et al 2017). Total soluble sugar (fructose, glucose and sucrose) in leaves of 17 day ex vitro adapted tobacco plants (acclimatized under 3% sucrose) was peaked, whereas it was undetected in sugar-free plantlets (Haisel et al 2001).…”

Section: Discussionmentioning

confidence: 99%

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In vitro acclimatization of Curcuma longa under controlled iso-osmotic conditions

Chintakovid

Tisarum

Samphumphuang

et al. 2021

Plant Biotechnology

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In vitro acclimatization has been validated as the successful key to harden the plantlets before transplanting to ex vitro conditions. In the present study, we investigated the potential of different sugar types (glucose, fructose, galactose, sucrose) in regulating morphological, physiological and biochemical strategies, survival percentage and growth performance, and rhizome traits of turmeric under iso-osmotic potential. Leaf greenness (SPAD value) in acclimatized plantlets (4% glucose; −1.355 MPa osmotic potential) of 'ST018' was retained and greater than in 'PB009' by 1.69-fold, leading to maintain high F v /F m (maximum quantum yield of PSII), Φ PSII (photon yield of PSII) and P n (net photosynthetic rate) levels, and retained shoot height, leaf length, leaf width, shoot fresh weight and shoot dry weight after one month upon transplanting to ex vitro conditions. In addition, P n , C i (intracellular CO 2), g s (stomatal conductance) and E (transpiration rate) in acclimatized plantlets (6% sucrose; −1.355 MPa osmotic potential) of 'PB009' were stabilized as physiological adapted strategies, regulating the shoot and root growth and fresh and dry weights of mini-rhizome. Interestingly, the accumulation of total curcuminoids in mini-rhizome derived from 6% sucrose acclimatized plantlets of 'ST018' was greater than in 'PB009' by 3.76-fold. The study concludes that in vitro acclimation of turmeric 'PB009' and 'ST018' using 6% sucrose and 4% glucose, respectively, promoted percent survival, physiological adaptations, and overall growth performances under greenhouse conditions.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

In vitro acclimatization of Curcuma longa under controlled iso-osmotic conditions

Chintakovid

Tisarum

Samphumphuang

et al. 2021

Plant Biotechnology

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“…2). Lembrechts et al (2017) com a bromélia Guzmania "Hilda" crescida in vitro com 5, 12,5, 25 e 40 g.L -1 de sacarose demonstraram que, durante a etapa de aclimatização, as plantas oriundas dos meios nutritivos com maiores concentrações deste carboidrato dependem da quebra dos açúcares e amido acumulados em suas folhas para a sua sobrevivência e crescimento. Entretanto, as plantas obtidas dos meios com menores adição de sacarose, e consequentemente menor acúmulo de açúcares e amido nas folhas, tiveram a fotossíntese favorecida, já que esta não é inibida por feedback mediado pelos açúcares.…”

Section: Resultsunclassified

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

et al. 2020

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Resumo A deiscência não simultânea dos frutos de bromélias deve ser considerada para fins de propagação por sementes. O objetivo deste estudo foi avaliar o melhor método para produção de plantas da bromélia imperial considerando a colheita simultânea das sementes de diferentes posições do escapo floral no período da deiscência dos frutos da base e seu armazenamento para posterior uso na obtenção de plantas. As sementes foram colhidas da base, meio e topo do escapo floral e armazenadas por 26, 61, 154 e 213 dias a 10 °C e a 25 °C. Foram avaliadas a emergência de plantas em gerbox com papel de filtro e a germinação e crescimento in vitro com 10, 20 e 30 g.L-1 de sacarose. As sementes colhidas da base e armazenadas por até dois meses a 10 °C foram as que apresentaram maior porcentagem de emergência de plantas (57%) e a melhor concentração de sacarose para germinação in vitro foi de 10 g.L-1 e para o crescimento, 20 g.L-1. Considerando-se a vulnerabilidade desta bromélia, o aproveitamento, ao mesmo tempo, das sementes de todo o escapo floral pode ser uma estratégia de conservação.

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“…Glucose also has a major role in metabolism, as it may serve as a bridge between carbohydrate and phytohormone signaling (Hartig & Beck, 2006). In addition, glucose has been highlighted as a signaling molecule in various processes associated with growth and development, such as germination, hypocotyl elongation, cotyledon expansion and leaf development (Lembrechts, Ceusters, Proft, & Ceusters, 2017). Hexoses and sucrose are generally associated with different seed development stages.…”

Section: Sugar Contentsmentioning

confidence: 99%

Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard

Conti

Corredor-Prado

Roecker

et al. 2018

Acta Sci. Biol. Sci.

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Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indoleacetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones and leaf primordia formation. Sucrose, fructose and glucose presence in this study is related to cell signaling. Thus, hormonal signals and carbohydrates alter metabolism, triggering PLBs initiation and development in C. tigrina.

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Sugar and starch dynamics in the medium-root-leaf system indicate possibilities to optimize plant tissue culture

Cited by 24 publications

References 25 publications

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

<b>Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of <i>Cattleya tigrina</i> A. Richard

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