Sugar Composition of Lipopolysaccharides of Family <i>Vibrionaceae</i>

Hisatsune, Kazuhito; Kondo, Seiichi; Iguchi, Takehiro; Machida, Masaaki; Asou, Shinobu; Inaguma, Makoto; Yamamoto, Fumihiro

doi:10.1111/j.1348-0421.1982.tb00209.x

Cited by 49 publications

(7 citation statements)

References 45 publications

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“…The sugar composition of the O-antigenic lipopolysaccharides (LPS) isolated from all 12 has been determined (5)(6)(7)18). On the basis of the sugar composition, LPS of the 12 of V. parahaemolyticus was divided into nine chemotypes, because O3, O5 and O11 LPS belong to the same chemotype and O7 and 012 LPS to another chemotype (5,9).…”

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confidence: 99%

Lipopolysaccharide Isolated from a New O‐Antigenic Form (O13) of Vibrio parahaemolyticus

Hisatsune

Iguchi

Haishima

et al. 1993

Microbiology and Immunology

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: The chemical properties of a lipopolysaccharide (LPS) isolated from a new O-antigenic form (O13) of Vibrio parahaemolyticus were investigated. The LPS contained glucose, galactose, L-glycero-D-manno-heptose and glucosamine. 2-Keto-3-deoxy-octonate (KDO) was not detected in the LPS by the periodate-thiobarbituric acid test (Weissbach's reaction) under conventional hydrolysis conditions. Instead, phosphorylated KDO (X1 and X2) was found in its strong-acid hydrolysate. This sugar composition was identical to that of V. parahaemolyticus O3, O5and O11 LPS, indicating that, based on the sugar comnosition. O13 LPS belongs to Chemotvne 111 to which and 011 belong.In addition, structural study demonstrated the presence of KDO 4-phosphate in its inner-core region.

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confidence: 99%

Lipopolysaccharide Isolated from a New O‐Antigenic Form (O13) of Vibrio parahaemolyticus

Hisatsune

Iguchi

Haishima

et al. 1993

Microbiology and Immunology

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“…Although most LPSs reported so far contain KDO it is known that Vibrio cholerae LPS lacks KDO (10 , 11). Recently, it was reported that the LPSs of most of the family Vibrionaceae also lack KDO (9). We also reported that LPSs from several marine photobacteria belonging to the family Vibrionaceae had undetectable or only small amounts of KDO in their constituents (12,13).…”

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confidence: 75%

Chemical Properties of Thiobarbituric Acid‐Positive Substances Released from Photobacterial Lipopolysaccharides during Acid Hydrolysis

Kuwae

Kurata

Sakagishi

1985

Microbiology and Immunology

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Lipopolysaccharides (LPSs) are located in the outer membranes of gramnegative bacterial cell walls and exhibit various biological activities (7,14) . Many LPSs have been isolated from various gram-negative bacteria and studied extensively with respect to their structure and biological activities. Most of these LPSs consist of three regions, namely, 1) an O-side chain of repeated sugar units which are specific for the bacterial species or strain, 2) a core region, and 3) lipid A which plays a role in most of their biological activities. Polysaccharides (containing the O-side chain and the core region) and lipid A in their LPSs are linked through 2-keto-3-deoxyoctonate (KDO) (6, 16). The LPS core region contains two or three residues of KDO , a unique acidic sugar, as one of its essential components. Although most LPSs reported so far contain KDO it is known that Vibrio cholerae LPS lacks KDO (10 , 11). Recently, it was reported that the LPSs of most of the family Vibrionaceae also lack KDO (9). We also reported that LPSs from several marine photobacteria belonging to the family Vibrionaceae had undetectable or only small amounts of KDO in their constituents (12, 13). More recently, Brade and coworkers reported that both V. cholerae Ogawa and Inaba LPSs released thiobarbituric acid (TBA)-positive substances under strong acid hydrolysis (3) and that these substances were identified as .In the present study, we reexamined marine photobacterial LPSs to clarify whether they contain TBA-positive substances, and our results indicate that TBApositive substances are released from all photobacterial LPSs tested under strong acid hydrolysis. The chemical properties of these TBA-positive substances , which were partially characterized, are described.The source, species and tentative designation of the marine photobacteria used in this study are listed in Table 1.Each LPS was isolated from the corresponding photobacterium by Westphal's hot phenol-water method (21), dialyzed, purified by repeated ultracentrifugation and lyophilized, and the chemical composition of each LPS was determined as described previously (13). These purified LPSs consisted of 35 % to 65 % sugar , 112 1

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“…KDO was estimated by the method of Weissbach & Hurwitz (1959). Degraded polysaccharide (DPS) was prepared from the supernatant of a weak-acid hydrolysate (S% acetic acid, 100 "C, 3 h) of LPS (Hisatsune et al, 1985). Gel chromatography was performed with Sephadex G-50 and G-10 columns (1.6 x 100 cm) using pyridine/acetic acid/water (10 :4: 1000, by vol.)…”

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confidence: 99%

“…In the elution profiles of 'DPS' of either Ogawa or Inaba LPS in Sephadex G-50 gel chromatography using pyridine/acetate buffer (pH 7.0) as eluent (Hisatsune et al, 1985), typically three peaks are observed : the first peak (fraction I) corresponds to the polymeric 0-polysaccharide side chain, namely an a( 1 +2)-linked homopolymer of perosamine, with the attached core stubs, the second peak (fraction 11) to the core oligosaccharide, and the third peak (fraction 111) to low-molecular-mass fragments, namely fructose and inorganic phosphate. Sephadex G-50 gel filtration of 'DPS' prepared from either Ogawa or Inaba 'oxidized LPS' was carried out.…”

Section: Efect Of Periodate Oxidation On Chemical Structure Of Lpsmentioning

confidence: 99%

Relationship between Structure and Antigenicity of O1 Vibrio cholerae Lipopolysaccharides

Hisatsune

Hayashi²,

Haishima³

et al. 1989

Microbiology

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The relationship between the release of fructose from 0 1 Vibrio cholerae lipopolysaccharides (LPS) by dilute acetic acid hydrolysis and the decrease in their antigenicity was examined. Decrease in the antigenicity of LPS was not parallel with the release of fructose, and occurred very much later than the latter. Periodate oxidation of LPS resulted in the total elimination of the fructose and glucose, and two-thirds of the heptose constituents, but no difference in the antigenicity of LPS was observed before and after oxidation. These findings indicate that the fructose present in 01 V . cholerae LPS is not substantially involved in their specific antigenicity. In the 01 V. cholerae LPS, the fructose is in the branch structure, most probably in the core region. I N T R O D U C T I O NA large variety of neutral and amino sugars are present as components in lipopolysaccharides (LPS) of Gram-negative bacteria. However, the neutral sugar fructose occurs only rarely as an LPS component (Wilkinson, 1577;Kenne & Lindberg, 1983). Recently, a fructose-containing polysaccharide was reported to exist in the capsular K4 antigen of Escherichia coli 0 5 : K4 : H4 (Rodriguez et al., 1988). In a previous study, we demonstrated that, among the vibrios studied, 01 Vibrio cholerae was the only group for which all strains contained fructose in LPS (Kondo et al., 1988).The role of fructose in the serology of LPS of 0 1 V . cholerae is of interest. Since heating of 0 1 V . cholerae LPS in dilute acetic acid at 100°C results in release of fructose from LPS and concomitant loss of their antigenicity, it has been suspected that fructose is essentially involved in the antigenicity of LPS of this vibrio (Redmond & Korsch, 1973). On heating of LPS of most Gram-negative bacteria, free 2-keto-3-deoxyoctonate (KDO), a characteristic sugar constituent of LPS, is released. However, when 01 V . cholerae LPS is treated in this way, release of KDO does not occur; instead, fructose is released. In the LPS molecules of most Gram-negative bacteria, KDO connects the polysaccharide portion and lipid A, and it has been suggested that in the LPS molecule of 0 1 V . cholerae, KDO in this position may be replaced by fructose (Jann et al., 1973). However, results obtained by Kaca et al. (1986), studying the effect of removal of D-fructose on the antigenicity of LPS from a rough mutant of V . cholerae Ogawa, indicated that D-fructose does not link the polysaccharide and lipid A portion, but that fructose is present as a branch. It was further shown by passive haemolysis inhibition that the release of D-fructose paralleled the exposure of a new common antigenic determinant (Brade & Galanos, 1983a, b) cryptic in LPS.In the present study, the relationship between the release of fructose from 0 1 V . cholerae LPS by dilute acetic acid hydrolysis and the decrease in their antigenicity was examined using LPS Abbreviations : DPS, degraded polysaccharide ; KDO, 2-keto-3-deoxyoctonate (3-deoxy-~-manno-2-octulosonic acid; PHI, passive haemolysis inhibition.0001-5280 ...

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Sugar Composition of Lipopolysaccharides of Family Vibrionaceae

Cited by 49 publications

References 45 publications

Lipopolysaccharide Isolated from a New O‐Antigenic Form (O13) of Vibrio parahaemolyticus

Lipopolysaccharide Isolated from a New O‐Antigenic Form (O13) of Vibrio parahaemolyticus

Chemical Properties of Thiobarbituric Acid‐Positive Substances Released from Photobacterial Lipopolysaccharides during Acid Hydrolysis

Relationship between Structure and Antigenicity of O1 Vibrio cholerae Lipopolysaccharides

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