Sugar homeostasis mediated by cell wall invertase <scp>GRAIN INCOMPLETE FILLING</scp> 1 (<scp>GIF1</scp>) plays a role in pre‐existing and induced defence in rice

Sun, Li; Yang, Donglei; Kong, Yu; Chen, Ying; Li, Xiaozun; Zeng, Longjun; Li, Qun; Wang, Ertao; He, Zhonghu

doi:10.1111/mpp.12078

Cited by 70 publications

(54 citation statements)

References 63 publications

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“…Moreover, we observed upregulation of invertase (cwINV2), whose function is to irreversibly hydrolyze sucrose into glucose and fructose. It has been described that upregulation of cwINV during infection with pathogens allows the induction of several PR genes [109–113]. Particularly, loss of function of a rice cwINV ortholog gene (GIF1) caused hyper susceptibility to postharvest fungal pathogens, while constitutive expression of rice GIF1 increased resistance to fungi and bacteria [113].…”

Section: Resultsmentioning

confidence: 99%

“…It has been described that upregulation of cwINV during infection with pathogens allows the induction of several PR genes [109–113]. Particularly, loss of function of a rice cwINV ortholog gene (GIF1) caused hyper susceptibility to postharvest fungal pathogens, while constitutive expression of rice GIF1 increased resistance to fungi and bacteria [113]. In addition, the metabolic changes in sugar species and concentration provided by an invertase and repression of photosynthesis lead to transition from source to sink tissues.…”

Section: Resultsmentioning

confidence: 99%

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Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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Sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. Crop productivity is negatively affected by Acidovorax avenae subsp avenae (Aaa), which causes the red stripe disease. Little is known about the molecular mechanisms triggered in response to the infection. We have investigated the molecular mechanism activated in sugarcane using a RNA-seq approach. We have produced a de novo transcriptome assembly (TR7) from sugarcane RNA-seq libraries submitted to drought and infection with Aaa. Together, these libraries present 247 million of raw reads and resulted in 168,767 reference transcripts. Mapping in TR7 of reads obtained from infected libraries, revealed 798 differentially expressed transcripts, of which 723 were annotated, corresponding to 467 genes. GO and KEGG enrichment analysis showed that several metabolic pathways, such as code for proteins response to stress, metabolism of carbohydrates, processes of transcription and translation of proteins, amino acid metabolism and biosynthesis of secondary metabolites were significantly regulated in sugarcane. Differential analysis revealed that genes in the biosynthetic pathways of ET and JA PRRs, oxidative burst genes, NBS-LRR genes, cell wall fortification genes, SAR induced genes and pathogenesis-related genes (PR) were upregulated. In addition, 20 genes were validated by RT-qPCR. Together, these data contribute to a better understanding of the molecular mechanisms triggered by the Aaa in sugarcane and opens the opportunity for the development of molecular markers associated with disease tolerance in breeding programs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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“…The roles of CWIN in plant responses to biotic stresses have been widely acknowledged largely based on transgenic studies (Benhamou et al, 1991;Herbers et al, 1996;Essmann et al, 2008;Sun et al, 2014). Its potential role in abiotic stress responses, however, remains less certain.…”

Section: Discussionmentioning

confidence: 99%

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

2016

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Reduced cell wall invertase (CWIN) activity has been shown to be associated with poor seed and fruit set under abiotic stress. Here, we examined whether genetically increasing native CWIN activity would sustain fruit set under long-term moderate heat stress (LMHS), an important factor limiting crop production, by using transgenic tomato (Solanum lycopersicum) with its CWIN inhibitor gene silenced and focusing on ovaries and fruits at 2 d before and after pollination, respectively. We found that the increase of CWIN activity suppressed LMHS-induced programmed cell death in fruits. Surprisingly, measurement of the contents of H 2 O 2 and malondialdehyde and the activities of a cohort of antioxidant enzymes revealed that the CWINmediated inhibition on programmed cell death is exerted in a reactive oxygen species-independent manner. Elevation of CWIN activity sustained Suc import into fruits and increased activities of hexokinase and fructokinase in the ovaries in response to LMHS. Compared to the wild type, the CWIN-elevated transgenic plants exhibited higher transcript levels of heat shock protein genes Hsp90 and Hsp100 in ovaries and HspII17.6 in fruits under LMHS, which corresponded to a lower transcript level of a negative auxin responsive factor IAA9 but a higher expression of the auxin biosynthesis gene ToFZY6 in fruits at 2 d after pollination. Collectively, the data indicate that CWIN enhances fruit set under LMHS through suppression of programmed cell death in a reactive oxygen species-independent manner that could involve enhanced Suc import and catabolism, HSP expression, and auxin response and biosynthesis.

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“…In rice, constitutive overexpression of the cell wall invertase gene GRAIN INCOMPLETE FILLING 1 enhances resistance against both the bacteria Xanthomonas oryzae pv. Oryzae and the fungus Magnaporthe oryzae (Sun et al, 2014). In tobacco, RNA interference of CWIN genes LIN6 and LIN8 results in an increased susceptibility to the oomycete Phytophtora nicotianae (Essmann et al, 2008a).…”

Section: Introductionmentioning

confidence: 99%

Targeting the AtCWIN1 Gene to Explore the Role of Invertases in Sucrose Transport in Roots and during Botrytis cinerea Infection

et al. 2016

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Cell wall invertases (CWIN) cleave sucrose into glucose and fructose in the apoplast. CWINs are key regulators of carbon partitioning and source/sink relationships during growth, development and under biotic stresses. In this report, we monitored the expression/activity of Arabidopsis cell wall invertases in organs behaving as source, sink, or subjected to a source/sink transition after infection with the necrotrophic fungus Botrytis cinerea. We showed that organs with different source/sink status displayed differential CWIN activities, depending on carbohydrate needs or availabilities in the surrounding environment, through a transcriptional and posttranslational regulation. Loss-of-function mutation of the Arabidopsis cell wall invertase 1 gene, AtCWIN1, showed that the corresponding protein was the main contributor to the apoplastic sucrose cleaving activity in both leaves and roots. The CWIN-deficient mutant cwin1-1 exhibited a reduced capacity to actively take up external sucrose in roots, indicating that this process is mainly dependent on the sucrolytic activity of AtCWIN1. Using T-DNA and CRISPR/Cas9 mutants impaired in hexose transport, we demonstrated that external sucrose is actively absorbed in the form of hexoses by a sugar/H+ symport system involving the coordinated activity of AtCWIN1 with several Sugar Transporter Proteins (STP) of the plasma membrane, i.e., STP1 and STP13. Part of external sucrose was imported without apoplastic cleavage into cwin1-1 seedling roots, highlighting an alternative AtCWIN1-independent pathway for the assimilation of external sucrose. Accordingly, we showed that several genes encoding sucrose transporters of the plasma membrane were expressed. We also detected transcript accumulation of vacuolar invertase (VIN)-encoding genes and high VIN activities. Upon infection, AtCWIN1 was responsible for all the Botrytis-induced apoplastic invertase activity. We detected a transcriptional activation of several AtSUC and AtVIN genes accompanied with an enhanced vacuolar invertase activity, suggesting that the AtCWIN1-independent pathway is efficient upon infection. In absence of AtCWIN1, we postulate that intracellular sucrose hydrolysis is sufficient to provide intracellular hexoses to maintain sugar homeostasis in host cells and to fuel plant defenses. Finally, we demonstrated that Botrytis cinerea possesses its own functional sucrolytic machinery and hexose uptake system, and does not rely on the host apoplastic invertases.

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Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 (GIF1) plays a role in pre‐existing and induced defence in rice

Cited by 70 publications

References 63 publications

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

Targeting the AtCWIN1 Gene to Explore the Role of Invertases in Sucrose Transport in Roots and during Botrytis cinerea Infection

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