Sugar-Induced Disintegration of Layer-by-Layer Assemblies Composed of Concanavalin A and Glycogen

Sato, Katsuhiko; Imoto, Yoko; Sugama, Jun; Seki, Shunsuke; Inoue, Hiroyuki; Odagiri, Tsuyoshi; Hoshi, Tomonori; Anzai, Jun Ichi

doi:10.1021/la048059x

Cited by 128 publications

(113 citation statements)

References 29 publications

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“…11 The quartz slide was first treated in diclorodimethylsilane (10% solution in toluene) overnight at room temperature, to make the surface hydrophobic, and was then washed with toluene, acetone, and distilled water.…”

Section: Preparation Of Con A-polymer Multilayer Filmsmentioning

confidence: 99%

“…The loading of Con A in the film is nearly one third of the value observed for the Con A/glycogen multilayer film. 11 This may originate from the different molecular geometry between PV-MA and glycogen; glycogen is a branched polysaccharide composed of D-glucose units, while PV-MA consists of a linear polymer chain without branching.…”

Section: Preparation Of Con A-polymer Layered Filmsmentioning

confidence: 99%

“…It is noted that not only glycogen, 10,11 but also the synthetic polymer (i.e., PV-MA) can also be used for constructing the sugar-sensitive Con A assembly. This in turn suggests that the performance characteristics of the sugar-sensitive Con A assembly could be further improved by suitably modiying the chemistry of the polymer.…”

Section: Sugar-induced Disintegration Of Con A/pv-ma Filmmentioning

confidence: 99%

“…9 We have already reported on the preparation of stimuli-sensitive multilayer films that can be disintegrated by being exposed to sugars. 10,11 Stimuli-sensitive multilayer films were fabricated using concanavalin A (Con A) and glycogen. Con A is a lectin protein (molecular weight; 104000) found in Jack bean and is known to contain four identical binding sites to sugars, such as D-mannose and D-glucose (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…2. 10,11 The present paper reports on sugar-sensitivity of Con A multilayer films prepared by a layer-by-layer deposition technique using sugar-substituted synthetic polymers in place of glycogen.…”

Section: Introductionmentioning

confidence: 99%

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Sugar-Sensitive Thin Films Composed of Concanavalin A and Sugar-Bearing Polymers

2005

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Layered thin films composed of concanavalin A (Con A) and sugar-bearing polymers were prepared by a layer-by-layer deposition of Con A and the polymer on a solid surface. The sugar-induced disintegration was studied. Con A-polymer layered films could be successfully prepared using a maltose-bearing polymer (PV-MA), while melibiose-and glucosebearing polymers (PV-MEA and PV-G) did not afford a layered film, due to a weak affinity of PV-MEA and PV-G to Con A. The Con A/PV-MA layered film was stable in pH 7 and 8 solutions, while in a pH 6 medium the film was slightly unstable. The Con A/PV-MA film was disintegrated upon the addition of sugars in solution owing to a preferential binding of the sugars to the binding site of Con A in the film. The disintegration rate was dependent on the type of sugar and its concentration. The Con A/PV-MA film was disintegrated rapidly upon the addition of methyl α-Dmannopyranoside, while the rate was slower upon the addition of the same concentration of D-mannose, D-glucose and methyl α-D-glucopyranoside. The present system may be useful for constructing sensitive devices that can release a drug or other functional molecules in response to sugars.

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Section: Preparation Of Con A-polymer Multilayer Filmsmentioning

confidence: 99%

Section: Preparation Of Con A-polymer Layered Filmsmentioning

confidence: 99%

Section: Sugar-induced Disintegration Of Con A/pv-ma Filmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sugar-Sensitive Thin Films Composed of Concanavalin A and Sugar-Bearing Polymers

2005

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Microgels in Drug Delivery

Malmsten

2011

Microgel Suspensions

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Layer‐by‐Layer Self‐Assembled Multilayer Stimuli‐Responsive Polymeric Films

Zhai

2011

Handbook of Stimuli‐Responsive Materials

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IntroductionMultilayer polymeric films fabricated through the layer-by-layer (LBL) self-assembly technique are able to integrate stimuli-responsive materials into the films through various intermolecular interactions. The ease of the fabrication process and the excellent control of the film composition and morphology, combined with the capability of coating substrates conformally, offers a wide range of multilayer films that change film properties such as permeability, wettability, color, and solubility upon external stimuli. This chapter reviews the methods of building LBL multilayer films and the approaches to control the structures of the films. These smart films provide numerous applications in controlled drug release, sensing, energy generation, and flow regulation with their unique response to external stimuli including pH and temperature change, photo irradiation, application of electrical fields, and specific molecular interactions.Stimuli-responsive coatings, also known as smart coatings, are able to change their properties upon external stimuli including temperature, pH, salt concentration, light, presence of specific molecules, and other factors. The capability of changing surface behavior in an accurate and predictable manner has generated numerous applications ranging from chemical sensing, separations, and drug delivery to various bioapplications. To name a few, smart coatings with tunable hydrophilic/hydrophobic wettability have been used in the development of microand nanofluidic devices, self-cleaning and antifog surfaces, and sensor devices [1-3]. Photochemical-responsive coatings have been applied to build a surface that releases nitric oxide [4] over nanometer length scales for photochemotherapeutic applications, and a surface that can be photoactivated for spatiotemporal control of cell adhesion during cell cultivation [5,6]. The control of the properties of these smart coatings can be achieved through the stimuli-responsive materials forming self-assembled monolayers (SAMs) and polymer films. Among various approaches to fabricate responsive polymer films, multilayered polymer films, generally referred as polyelectrolyte multilayer (PEM) films, fabricated through the LBL assembly technique, have been proven to be effective smart coatings with Handbook of Stimuli-Responsive Materials. Edited by Marek W. Urban

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Sugar-Induced Disintegration of Layer-by-Layer Assemblies Composed of Concanavalin A and Glycogen

Cited by 128 publications

References 29 publications

Sugar-Sensitive Thin Films Composed of Concanavalin A and Sugar-Bearing Polymers

Sugar-Sensitive Thin Films Composed of Concanavalin A and Sugar-Bearing Polymers

Microgels in Drug Delivery

Layer‐by‐Layer Self‐Assembled Multilayer Stimuli‐Responsive Polymeric Films

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