Sugar sensing and Ca2+–calmodulin requirement in Vitis vinifera cells producing anthocyanins

Vitrac, Xavier; Larronde, Fabienne; Krisa, Stéphanie; Décendit, Alain; Deffieux, Gérard; Mérillon, Jean‐Michel

doi:10.1016/s0031-9422(99)00620-2

Cited by 177 publications

(135 citation statements)

References 34 publications

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“…Sugar signaling associated with anthocyanin accumulation is linked to several signaling pathways such as those triggered by light, CK, ethylene, JA, and ABA, which might be interconnected with Ca 2+ signaling by as yet unknown mechanisms (Vitrac et al, 2000). Although light is known to be a crucial factor for the hormonal regulation of anthocyanin biosynthesis, the details of the interactions between sugar, light and hormones remain to be elucidated.…”

Section: Resultsmentioning

confidence: 99%

Sugar-Hormone Cross-Talk in Anthocyanin Biosynthesis

Das

Shin

Choi

et al. 2012

Molecules and Cells

173

116

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Section: Resultsmentioning

confidence: 99%

Sugar-Hormone Cross-Talk in Anthocyanin Biosynthesis

Das

Shin

Choi

et al. 2012

Molecules and Cells

173

116

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“…Several cis-acting elements in the promoter of key genes regulating anthocyanin biosynthesis are activated by a series of signal transduction processes under the light and temperature receptors (Vitrac et al, 2000;Dela et al, 2003;Zhang et al, 2003). Transposon insertions and deletions in the promoter region of grape 1 This work was supported by the Doctoral Program Special Fund of the Ministry of Education in China (grant no.…”

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confidence: 99%

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

et al. 2013

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Varieties of the European pear (Pyrus communis) can produce trees with both red-and green-skinned fruits, such as the Max Red Bartlett (MRB) variety, although little is known about the mechanism behind this differential pigmentation. In this study, we investigated the pigmentation of MRB and its green-skinned sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes the MRB-G sport. Transcript levels of PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), the key structural gene in anthocyanin biosynthesis, paralleled the change of anthocyanin concentration in both MRB and MRB-G fruit. We cloned the PcMYB10 gene, a transcription factor associated with the promoter of PcUFGT. An investigation of the 2-kb region upstream of the ATG translation start site of PcMYB10 showed the regions 2604 to 2911 bp and 21,218 to 21,649 bp to be highly methylated. A comparison of the PcMYB10 promoter methylation level between the MRB and MRB-G forms indicated a correlation between hypermethylation and the green-skin phenotype. An Agrobacterium tumefaciens infiltration assay was conducted on young MRB fruits by using a plasmid constructed to silence endogenous PcMYB10 via DNA methylation. The infiltrated fruits showed blocked anthocyanin biosynthesis, higher methylation of the PcMYB10 promoter, and lower expression of PcMYB10 and PcUFGT. We suggest that the methylation level of PcMYB10 is associated with the formation of the greenskinned sport in the MRB pear. The potential mechanism behind the regulation of anthocyanin biosynthesis is discussed.

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“…The idea that CDPKs could be involved in the regulation of secondary metabolite biosynthesis was initially developed by the use of common protein kinase inhibitors and CDPK antagonists that decreased accumulation of phytoalexins in different plants (Choi and Bostock, 1994;Vitrac et al, 2000). Identification of specific CDPK isoforms in French bean and Arabidopsis that could phosphorylate phenylalanine ammonia lyase (PAL), a key enzyme in defense responses, provided an apparent link between secondary metabolism and stress perception via CDPK (Allwood et al, 1999(Allwood et al, , 2002Cheng et al, 2001).…”

mentioning

confidence: 99%

Induction of anthraquinone biosynthesis in Rubia cordifolia cells by heterologous expression of a calcium‐dependent protein kinase gene

Shkryl

Veremeichik

Bulgakov

et al. 2011

Biotech & Bioengineering

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Calcium-dependent protein kinases (CDPKs) play an important role in plant cell responses to stress and pathogenic attack. In this study, we investigated the effect of heterologous expression of the Arabidopsis CDPK gene, AtCPK1, on anthraquinone production in transgenic Rubia cordifolia cells. AtCPK1 variants (a constitutively active, Ca 2þ -independent form and a non-active form used as a negative control) were transferred to callus cells by agrobacterial transformation. Overexpression of the constitutively active, Ca 2þ -independent form in R. cordifolia cells caused a 10-fold increase in anthraquinone content compared with non-transformed control cells, while the nonactive form of AtCPK1 had no effect on anthraquinone production. Real-time PCR measurements showed that the activation of anthraquinone biosynthesis in transgenic calli correlated with the activation of isochorismate synthase gene expression. The activator effect of AtCPK1 was stable during prolonged periods of transgenic cell cultivation (more than 3 years) and the transgenic cultures exhibited high growth. Our results provide the first evidence that a CDPK gene can be used for the engineering of secondary metabolism in plant cells.

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Sugar sensing and Ca2+–calmodulin requirement in Vitis vinifera cells producing anthocyanins

Cited by 177 publications

References 34 publications

Sugar-Hormone Cross-Talk in Anthocyanin Biosynthesis

Sugar-Hormone Cross-Talk in Anthocyanin Biosynthesis

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

Induction of anthraquinone biosynthesis in Rubia cordifolia cells by heterologous expression of a calcium‐dependent protein kinase gene

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