SUGARS MEDIATED GERMINATION IN SPORES OF Bacillus megaterium

Tehri, Nimisha; Kumar, Naresh; Yadav, Avinash; Raghu, H. V.; Singh, Namita Ashish

doi:10.9735/0975-5276.10.3.1058-1061

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…In this regard, it was earlier demonstrated that the difference in outgrowth time of two single spores is proportional to their difference in germination time. Depending on the nutrient of the germination medium or the origin of spores, germination times can vary considerably [17,18]. These differences explain why colonies from outgrowth of spores in a population appear at different times during incubation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sporulation Medium Content in Carbohydrates, Lipids, and Proteins on Outgrowth of Bacillus spp Spores Activated with Heat and Ethanol Solution

Tong

Kamdem²,

Ngougue³

et al. 2022

JAMB

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Aims: evaluate the effect of the nutrient content of the medium on the sporulation percentage and the efficiency of ethanolic activation on the outgrowth of Bacillus spp. Place and Duration: Department of Microbiology of the University of Yaoundé I between june 2020 and September 2021. Methodology: In order to contribute to the understanding of the effect of the environment of spore origin on the ability to sporulate and outgrow in foods, spores of Bacillus cereus and Bacillus subtilis were produced in different media differenciated by the carbohydrates, lipids and proteins composition. Mathematical models predicting sporulation rates and germination yields of heat- and ethanol-activated spores were evaluated as a function of the factors selected. The reliability of these models was assessed by comparing the predicted values with those obtained in real food. Results: It was found that spores originating from high carbohydrates and proteins media had high sporulating and germination rates. It was found that the origin of the spores, mainly the carbohydrate and protein content, resulted in high sporulation rates and that spores from nutrient-rich media germinated faster than those from nutrient-poor media. Germination efficiency varied from strain to strain depending on the method of activation and the composition of the medium in which the spores were produced. The mathematical models obtained were validated in food matrices with correlation of predicted and observed values higher than 0.85. Conclusion: The ethanolic activation of spore varies depending on the strain and the nutrient content especially the fat content in sporulating medium. The proteins and carbohydrates have significant content for thermal activation.

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Section: Discussionmentioning

confidence: 99%

Effect of Sporulation Medium Content in Carbohydrates, Lipids, and Proteins on Outgrowth of Bacillus spp Spores Activated with Heat and Ethanol Solution

Tong

Kamdem²,

Ngougue³

et al. 2022

JAMB

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“…[30][31][32][33][34] Bacterial spores are robust, vegetative, and metabolically dormant, and are produced to survive the severe and adverse climatic conditions of starvation and stress. 132,133 Exposure to germinants induces the germination of spores, which are highly resistant to varying ranges of temperature, pressure, desiccation, ultraviolet radiation, pH extremes, and noxious chemicals, including hypochlorite, aldehydes, ethylene oxide, and several other extreme conditions. 132,134 In the presence of specific small molecule germinants such as L-Ala or other nutrients, spores interact via germinant-specific receptors to reactivate their metabolism and allow for vegetative growth in Bacillus spp.…”

Section: Prokaryote Production Of D-aasmentioning

confidence: 99%

Promising applications of D-amino acids in periprosthetic joint infection

et al. 2023

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Due to the rise in our aging population, a disproportionate demand for total joint arthroplasty (TJA) in the elderly is forecast. Periprosthetic joint infection (PJI) represents one of the most challenging complications that can occur following TJA, and as the number of primary and revision TJAs continues to rise, an increasing PJI burden is projected. Despite advances in operating room sterility, antiseptic protocols, and surgical techniques, approaches to prevent and treat PJI remain difficult, primarily due to the formation of microbial biofilms. This difficulty motivates researchers to continue searching for an effective antimicrobial strategy. The dextrorotatory-isoforms of amino acids (D-AAs) are essential components of peptidoglycan within the bacterial cell wall, providing strength and structural integrity in a diverse range of species. Among many tasks, D-AAs regulate cell morphology, spore germination, and bacterial survival, evasion, subversion, and adhesion in the host immune system. When administered exogenously, accumulating data have demonstrated that D-AAs play a pivotal role against bacterial adhesion to abiotic surfaces and subsequent biofilm formation; furthermore, D-AAs have substantial efficacy in promoting biofilm disassembly. This presents D-AAs as promising and novel targets for future therapeutic approaches. Despite their emerging antibacterial efficacy, their role in disrupting PJI biofilm formation, the disassembly of established TJA biofilm, and the host bone tissue response remains largely unexplored. This review aims to examine the role of D-AAs in the context of TJAs. Data to date suggest that D-AA bioengineering may serve as a promising future strategy in the prevention and treatment of PJI.

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