Suitability of Three Stains to Mark Shells of Concholepas Concholepas (Gastropoda) and Mesodesma Donacium (Bivalvia)

Riascos, José M.; Guzmán, Nury; Laudien, Jürgen; Heilmayer, Olaf; Oliva, Marcelo E.

doi:10.2983/0730-8000(2007)26[43:sotstm]2.0.co;2

Cited by 48 publications

(61 citation statements)

References 21 publications

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“…Linard et al (2011) reported no mortality for P. margaritifera at 0.15g/L. Other studies revealed that lower than 0.40g/L calcein concentration were not lethal for Nucella ostrina (Moran, 2000) and Mesodesma donacium (Riascos et al, 2007). Herrmann (2009) found exposure time (3h vs 6h) to calcein to produce slightly more mortality for D. hanleyanus than the actual calcein concentration used (from 4 to 6% mortality), and thus recommended calcein staining as a non-lethal method.…”

Section: Discussionmentioning

confidence: 99%

“…Calcein used as a CaCO 3 -stainer was successful by immersion as a method for age validation producing a concise bright lime-green (Riascos et al, 2007), Donax hanleyanus (Herrmann et al, 2009), Cerastoderma edule (Mahé, Bellamy, Lartaud, & de Rafelis, 2010), Pinctada margaritifera (Linard et al, 2011), Loripes lacteus (van der Geest, van Gils, van der Meer, Olff, & Piersma, 2011) and other bivalves (Rowley & Mackinnon, 1995 Regarding the calcein concentration impact on marking, our individuals produced better marking (100%) at the lower (0.4g/L) than higher concentration (73% for 0.7g/L), as well as, a better quality mark at the lesser concentration (more perfect and good marks). The 27% of oysters at the greater concentration that showed no marking, actually showed green fluorescence over the whole shell thickness, as if the greater concentration affected the complete aragonite layer.…”

Section: Discussionmentioning

confidence: 99%

“…Once laid down in the CaCO 3 growth-band, calcein emits a lime-green fluorescent color when exposed to blue light (Wilson, Beckman, & Dean, 1987;Heilmayer et al, 2005;Tada, Fujikura, Oguri, Kitazato, & Tanabe, 2010;Linard et al, 2011), which was shown highly advantageous and appropriate, over other methods, in aiding the determination of growth increments after initial calcein mark incorporation into the growingedge in certain bivalves (Kaehler & McQuaid, 1999;Fujikura et al, 2003;Heilmayer et al, 2005;Riascos, Guzman, Laudien, Heilmayer, & Oliva, 2007;Riascos, Heilmayer, Oliva, Laudien, & Arntz, 2008;Herrmann et al, 2009;Cáceres-Puig, Huato-Soberanis, Melo-Barrera, & Saucedo, 2011).…”

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Eficiencia de la tinción de bandas de crecimiento con calceina y azul de coomassie, para estimar la edad de jóvenes de Pinctada mazatlanica (Pterioida: Pteriidae)

Loría

Huato‐Soberanis

2014

RBT

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Age validation is the first step to determine shellfish species age determination. This information is vital for different inferential models used in marine ecosystem management activities. In spite that various validation techniques are used for marking carbon calcium structures, the calcein marking technique for oysters had never been used for age validation in Pinctada mazatlanica. Thus the objectives of this study included: the evaluation of calcein to mark a shell growing-edge, and the efficacy of Coomassie Blue staining on posterior shell growth, to produce visible micro growth-bands that would enable age validation of juvenile mother-ofpearl oysters. Oysters were collected and cultivated at The Perlas del Cortez S. de R. L. MI. pearl-farming operation, in Pichilingue, La Paz Bay, Baja California Sur, Mexico; a total of 36 oysters (shell height 11.5-36.4mm) were injected with calcein (0.125g/L), and another 50 oysters (shell height 14.8-42.7mm) were submersed in calcein (0.4 and 0.7g/L). Shell slices of calcein-marked oysters were posteriourly stained with Coomassie Blue R-25 for micro growth-band recognition. Our results showed that Calcein marking only worked by submersion and produced a concise bright lime-green florescent band along the growing-edge with clear boundaries for both concentrations. However, marks resulted better at the lower calcein concentration (0.4g/L) with more "perfect" and "good" marks on the growing-edge (p=0.0012). Commassie Blue staining technique was successful, and allowed to conclude that one micro growth-band was laid down per day, similar to other oyster species. Mean 15-d increment of shell growth height was slightly greater at the lower calcein concentration ( =0.735mm) than at the higher one ( =0.577mm) (not significant difference, p=0.198). Calcein marking of shell growingedges and Commassie Blue staining of posterior shell growth, as a method for age validation is recommended for shellfish shell growth-band counts. This will allow back-dating for estimation of very precise colonization dates, both spatially and temporally in future work. Rev. Biol. Trop. 62 (3): 957-968. Epub 2014 September 01.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Eficiencia de la tinción de bandas de crecimiento con calceina y azul de coomassie, para estimar la edad de jóvenes de Pinctada mazatlanica (Pterioida: Pteriidae)

Loría

Huato‐Soberanis

2014

RBT

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“…Thereafter, clams were incubated for 3 h in moderately aerated, filtered seawater containing 50 mg l À1 Calcein (Sigma, CAS 1461-15-0) to incorporate a non-deleterious fluorescent mark in the shell, that would allow the measurement of short-term (i.e. daily) growth increments (Riascos et al, 2007). Thereafter, clams were randomly assigned to a single factor (temperature), three-level (NTC, ENTC, LNTC) experimental design, with three replicate aquaria per level and 12 individuals of D. obesulus and 16 individuals of M. donacium per replicate.…”

Section: In Vitro-experimentsmentioning

confidence: 99%

Recurrent, thermally-induced shifts in species distribution range in the Humboldt current upwelling system

Carstensen¹,

Riascos²,

Heilmayer³

et al. 2010

Marine Environmental Research

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a b s t r a c tEl Niño-Southern Oscillation (ENSO) is a global climate variablility, which fundamentally influences environmental patterns of the Humboldt Current System (HCS) off Chile and Peru. The surf clams Donax obesulus and Mesodesma donacium are dominant and highly productive bivalves of exposed sandy beaches of the HCS. Existing knowledge indicates that El Niño (EN, warm phase of ENSO) and La Niña (LN, cold phase of ENSO) affect populations of both species in a different way, although understanding of the mechanisms underlying these effects is still lacking. The aim of this study was to test hypotheses attempting to explain field observations on the effect of strong EN or LN events by using controlled experimental conditions. Growth and mortality rates of both species were registered during a four-week experiment under EN temperature conditions, normal temperature conditions and LN temperature conditions. While D. obesulus exhibited reduced growth and higher mortality under LN conditions, M. donacium showed reduced growth and higher mortality under EN conditions. The results clearly indicate different temperature tolerance windows for each species, possibly reflecting the evolutionary origins of the Donacidae and Mesodesmatidae in regions with contrasting temperature regimes. These results provide experimental support for previous hypotheses suggesting that thermal tolerance is the driving factor behind observed changes in the species distributions of D. obesulus and M. donacium during the extreme phases of ENSO.

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“…Each group of 30 snails was further subdivided into groups of 5 and placed in mesh chambers which allowed the flow-through of seawater. Prior to exposure to acidified seawater, all snails were soaked for 24 h in a saltwater solution of calcein, a soluble fluorochrome which is incorporated into growing calcified structures and produces a fluorescent band which can be treated as a baseline for subsequent growth (Riascos et al, 2007). The snails were maintained in the three pH treatments for a total of 90 days, although during that time each tank was assigned a particular pH for only 30 days.…”

Section: Culture Of Biological Organismsmentioning

confidence: 99%

Technical Note: Maximising accuracy and minimising cost of a potentiometrically regulated ocean acidification simulation system

2015

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Abstract. This article describes a potentiometric ocean acidification simulation system which automatically regulates pH through the injection of 100 % CO 2 gas into temperaturecontrolled seawater. The system is ideally suited to longterm experimental studies of the effect of acidification on biological processes involving small-bodied (10-20 mm) calcifying or non-calcifying organisms. Using hobbyist-grade equipment, the system was constructed for approximately USD 1200 per treatment unit (tank, pH regulation apparatus, chiller, pump/filter unit). An overall tolerance of ±0.05 pH T units (SD) was achieved over 90 days in two acidified treatments (7.60 and 7.40) at 12 • C using glass electrodes calibrated with synthetic seawater buffers, thereby preventing liquid junction error. The performance of the system was validated through the independent calculation of pH T (12 • C) using dissolved inorganic carbon and total alkalinity data taken from discrete acidified seawater samples. The system was used to compare the shell growth of the marine gastropod Zeacumantus subcarinatus infected with the trematode parasite Maritrema novaezealandensis with that of uninfected snails at pH levels of 7.4, 7.6, and 8.1.

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Suitability of Three Stains to Mark Shells of Concholepas Concholepas (Gastropoda) and Mesodesma Donacium (Bivalvia)

Cited by 48 publications

References 21 publications

Eficiencia de la tinción de bandas de crecimiento con calceina y azul de coomassie, para estimar la edad de jóvenes de Pinctada mazatlanica (Pterioida: Pteriidae)

Eficiencia de la tinción de bandas de crecimiento con calceina y azul de coomassie, para estimar la edad de jóvenes de Pinctada mazatlanica (Pterioida: Pteriidae)

Recurrent, thermally-induced shifts in species distribution range in the Humboldt current upwelling system

Technical Note: Maximising accuracy and minimising cost of a potentiometrically regulated ocean acidification simulation system

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