Sulfated modification and immunomodulatory activity of water-soluble polysaccharides derived from fresh Chinese persimmon fruit

Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative

“…Through sulfated modification, the physicochemical properties and space structure of the polysaccharides is changed because of the groups with sulfuric acid space steric hindrance and electrostatic repulsion. In turn, increasing the degree of the sugar chain flexion and extension to improve the solubility in water, and causing the change of the biological activities (Wei, Wei, Cheng, & Zhang, 2012;Zhang, Lu, Zhang, Qin, & Zhang, 2010).…”

Structural characterization and biological activities of a novel polysaccharide from <i>Phyllanthus emblica </i>

“…The splenocytes proliferation was assessed by using MTT-based colorimetric assay as previously described (32). An aliquot of 100 μL of splenocytes was seeded into 96-well flat bottom microtitre plates, thereafter the polysaccharide (final concentration 25, 50, 100, 200 and 400 μg/mL) was added, giving a final volume of 200 μL.…”

Sulfated modification and antioxidant activity of exopolysaccahrides produced by Enterobacter cloacae Z0206

“…There are many methods to sulfate polysaccharides, such as sulfuric acid, sulfur trioxide (SO 3 )-Pyr, CSA-Pyr, and SO 3 -dimethylacetamide [9]. CSA-Pyr method is the most popular one due to advantages such as high DS, high yield and convenient production reclamation [3,20].…”

“…In this study, nine sulfated derivatives of EPS were prepared with CSA-Pyr method by varying sulfation conditions, which are the important effective factors such as the ratio of CSA-Pyr, reaction temperature and reaction time. Polysaccharides performed at different conditions result in sulfated polysaccharides with various DS and bioactivities [9]. The yield, DS and carbohydrate content of nine polymers are listed in Table 1.…”

“…Many studies have demonstrated that the biological activities of polysaccharides could be obviously improved by the sulfated method [7][8][9][10]. The sulfation of many natural polysaccharides could not only enhance water solubility but also change the chain conformation, resulting in alteration of their biological activities [11,12].…”