Sulfated Polysaccharides Derived from Dietary Seaweeds Increase the Esterase Activity of a Lymphocyte Tryptase, Granzyme A

Hirayasu, Hirofumi; Yoshikawa, Yumiko; Tsuzuki, Satoshi; Fushiki, Tohru

doi:10.3177/jnsv.51.475

Cited by 17 publications

(18 citation statements)

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“…1; Table 1). We also showed previously that inclusion of sulfated oligosaccharides (k-carrageenan, fucoidan, and heparin) enhanced rGrA-catalyzed hydrolysis of Na-benzyloxy-L-lysine thiobenzyl ester, a synthetic substrate commonly used for determination of GrA activity in vitro (Hirayasu et al 2005). Of these, k-carrageenan [(1,3)-b-D-galactose-2-sulfate-(1,4)-a-D-galactose-2,6-disulfate-polymer, which is found in seaweeds] showed the highest potency (Hirayasu et al 2005).…”

Section: Resultssupporting

confidence: 52%

“…We also showed previously that inclusion of sulfated oligosaccharides (k-carrageenan, fucoidan, and heparin) enhanced rGrA-catalyzed hydrolysis of Na-benzyloxy-L-lysine thiobenzyl ester, a synthetic substrate commonly used for determination of GrA activity in vitro (Hirayasu et al 2005). Of these, k-carrageenan [(1,3)-b-D-galactose-2-sulfate-(1,4)-a-D-galactose-2,6-disulfate-polymer, which is found in seaweeds] showed the highest potency (Hirayasu et al 2005). On the other hand, chondroitin sulfate A [a polymer of (1,4)-b-D-glucuronic acid-(1,3)-b-D-N-acetyl-galactosamine 6-sulfate, which is widely distributed in animal and plant tissues] had no effects (Hirayasu et al 2005).…”

Section: Resultssupporting

confidence: 52%

“…These findings suggest that GrA, besides its role in the killing of abnormal cells, also contributes to the pathogenesis of certain chronic inflammatory lung diseases. Previous studies indicated that sulfated oligosaccharides such as heparin modulate GrA activity in vitro (Simon et al 1990;Hirayasu et al 2005). In the present study, we investigated the effect of sulfated oligosaccharides on rGrA-induced detachment and IL-8 release in A549 cells.…”

Section: Introductionmentioning

confidence: 84%

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Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells

et al. 2008

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Granzyme A (GrA) is a lymphocyte serine protease that is believed to enter virus-infected cells and growing tumors and induce apoptosis. We found recently that recombinant rat GrA (rGrA) promotes detachment of and interleukin (IL)-8 release from alveolar epithelial A549 cells and suggested that this protease is involved in the pathogenesis of certain inflammatory lung diseases. In the present study, we found that k-carrageenan (a sulfated oligosaccharide constituting the cell walls of seaweeds) potently inhibits rGrA-induced detachment and IL-8 release of A549 cells. This sulfated oligosaccharide might be useful for suppressing the development of inflammatory lung diseases in which GrA is thought to be involved.

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Section: Resultssupporting

confidence: 52%

Section: Resultssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 84%

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Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells

et al. 2008

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“…Regardless, these findings suggest that thrombin is more effective than rGrA for promotion of IL-8 release in A549 cells. We have found that BLT-hydrolyzing activity of our rGrA is comparable to that of native GrA (Kam et al 2000;Hirayasu et al 2005). Therefore, the lower efficiency of rGrA compared with thrombin in the context of IL-8 release may not be attributed to the catalytic capacity of the recombinant enzyme.…”

Section: Resultsmentioning

confidence: 74%

“…-charged resin (HisLink TM resin, Promega, Madison, WI, USA) were performed as described previously (Hirayasu et al 2005(Hirayasu et al , 2007(Hirayasu et al , 2008Tsuzuki et al 2003). In order to obtain the active form, the purified rGrA was incubated with 2.0 units/mL recombinant enterokinase (Novagen, Madison, WI, USA) for 18 h at 22°C.…”

Section: Methodsmentioning

confidence: 99%

Granzyme A and thrombin differentially promote the release of interleukin-8 from alveolar epithelial A549 cells

et al. 2010

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Some of extracellular serine proteases with trypsin-like specificity of cleavage have been known to increase the release of inflammatory mediators from various cell types. For instance, two well-known trypsin-like serine proteases circulating in blood, granzyme A (GrA) and thrombin, have been found to promote interleukin (IL)-8 release from an alveolar epithelial A549 cell line. However, the mechanisms by which the proteases promote IL-8 release from the cells are not fully understood. In the present study, using A549 cells we found that (1) thrombin promoted IL-8 release from the cells via a mechanism partially involving activation of protease-activated receptor-1, a G-protein coupled receptor, whereas a recombinant form of GrA (rGrA) did it via a mechanism that does not involve the receptor activation; that (2) unlike rGrA, thrombin did not cause detachment and microtubule disruption of the cells; and that (3) the release of IL-8 induced by rGrA was inhibited in the presence of taxol, a microtubulestabilizing reagent, whereas that induced by thrombin was not. These findings suggest that rGrA and thrombin promote the release of IL-8 from A549 cells through distinct mechanisms.

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Microalgae in Biotechnological Application: A Commercial Approach

Khatoon

Pal

2015

Plant Biology and Biotechnology

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Microalgae are used as food, feed, and fodder and also used to produce a wide range of metabolites such as, proteins, carbohydrates, lipids, carotenoids, vitamins, fatty acids, sterols, etc. They are able to enhance the nutritional content of conventional food and feed preparations and hence positively affect humans and animal health including aquaculture animals. They also provide a key tool for phycoremediation of toxic metals and nanometal production. The use of microalgae in nanotechnology is a promising fi eld of research with a green approach. The use of genetically modifi ed algae for better production of different biotechnological compounds of interests is popular nowadays. Microalgal biomass production for sustainable biofuel production together with other high-value compounds in a costeffective way is the major challenge of algal biotechnologists. Microalgal biotechnology is similar to conventional agriculture but has received quite a lot of attention over the last decades, because they can reach substantially higher productivities than traditional crops and can use the wastelands and the large marine ecosystem. As history has shown, research studies on microalgae have been numerous and varied, but they have not always resulted in commercial applications. The aim of this review is to summarize the commercial applications of microalgae.

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Sulfated Polysaccharides Derived from Dietary Seaweeds Increase the Esterase Activity of a Lymphocyte Tryptase, Granzyme A

Cited by 17 publications

References 8 publications

Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells

Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells

Granzyme A and thrombin differentially promote the release of interleukin-8 from alveolar epithelial A549 cells

Microalgae in Biotechnological Application: A Commercial Approach

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