Sulfation Patterns and the Amounts of Chondroitin Sulfate in the Diamond Squid,<i>Thysanoteuthis rhombus</i>

Tamura, Jun’ichi; Arima, Kazuya; Imazu, Ayaka; Tsutsumishita, Nao; Fujita, Hiroyuki; Yamane, Manami; Matsumi, Yoshiaki

doi:10.1271/bbb.90037

Cited by 19 publications

(5 citation statements)

References 20 publications

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“…Although BMPs and HS are one of the effective factors for bone formation, clinical application of BMPs is not only difficult due to cost but also the result of the necessary supra physiological doses that increase the risk of side effects. Recently, a technique for extraction and purification of sqCS from diamond squid ( Thysanoteuthis rhombus ) has been established by Tamura et al [22]. Moreover, sqCS has highly stable properties and can be purified in large quantities at low cost.…”

Section: Introductionmentioning

confidence: 99%

Diamond Squid (Thysanoteuthis rhombus)-Derived Chondroitin Sulfate Stimulates Bone Healing within a Rat Calvarial Defect

et al. 2013

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Chondroitin sulfate (CS) has been suggested to be involved in bone formation and mineralization processes. A previous study showed that squid-derived CS (sqCS) has osteoblastogenesis ability in cooperation with bone morphogenetic protein (BMP)-4 in vitro. However, in vivo, osteogenic potential has not been verified. In this study, we created a critical-sized bone defect in the rat calvaria and implanted sqCS-loaded gelatin hydrogel sponges (Gel) into the defect with or without BMP-4 (CS/BMP/Gel and CS/Gel, respectively). At 15 weeks, bone repair rate of CS/Gel-treated defects and CS/BMP/Gel-treated defects were 47.2% and 51.1%, respectively, whereas empty defects and defects with untreated sponges showed significantly less bone ingrowth. The intensity of von Kossa staining of the regenerated bone was less than that of the original one. Mineral apposition rates at 9 to 10 weeks were not significantly different between all treatment groups. Although bone repair was not completed, sqCS stimulated bone regeneration without BMP-4 and without external mesenchymal cells or preosteoblasts. Therefore, sqCS is a promising substance for promotion of osteogenesis.

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Section: Introductionmentioning

confidence: 99%

Diamond Squid (Thysanoteuthis rhombus)-Derived Chondroitin Sulfate Stimulates Bone Healing within a Rat Calvarial Defect

et al. 2013

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“…By comparison, extraction from fish heads, bones, or other organs containing a higher content of GAGs than muscle tissue generally resulted in higher amounts of extracted CS. 36,37 The amounts of CS recovered from various diamond squid tissue types were between 0.021 and 3.482 μg/mg defatted tissue, 38 while another study 39 detected 0.11 μg/mg sulfated CS disaccharides in rat muscle. Therefore, the amounts recovered from salmon muscle tissue in this study were at levels comparable with those found in similar tissue of other animal species.…”

Section: ■ Results and Discussionmentioning

confidence: 98%

Characterization of Glycosaminoglycans in Gaping and Intact Connective Tissues of Farmed Atlantic Salmon (Salmo salar) Fillets by Mass Spectrometry

Jacobsen¹,

Shi

Chun

et al. 2019

ACS Omega

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In the Atlantic salmon (Salmo salar) aquaculture industry, gaping (the separation of muscle bundles from the connective tissue) is a major quality problem. This study characterized chondroitin sulfate (CS) and heparan sulfate (HS) in the connective tissue of intact and gaping salmon fillets from 30 salmon by mass spectrometry. Statistical difference was detected between gaping and intact tissues only when comparing pairwise samples from the same individual (n = 10). The gaping tissue had a lower content of monosulfated CS disaccharides (p = 0.027), and the relative distribution of CS disaccharides was significantly different (p < 0.05). The HS chains were short (average = 14.09, SD = 4.91), and the intact tissue seemed to have a more uniform HS chain structure compared to the gaping tissue. Time-series samples from the same individuals are recommended for future research to improve the understanding of reasons and implications of these differences.

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“…sal fin of the yellowfin sole (Limanda aspera) contains more than 1300 mg of CS per 100 g of dry defatted tissue, whereas other flatfish, such as the flathead flounder (Hippoglossoides dubius), contain less than 200 mg. Squid (Thysanoteuthis rhombus) contains a larger amount of CS (ca. 350 mg per 100 g of dry defatted tissue) in the skin than that in the mantle (2 mg per 100 g of dry defatted tissue) 6) . The CS in all tissues of several species of squid is characteristically composed of the CS-E-type 20 %, in contrast to other animals 4) .…”

Section: Naturally Occurring Cs Specifically Sulfated Polysaccharidesmentioning

confidence: 88%

Facile Synthesis of Biofunctional Oligosaccharides of Chondroitin Sulfate

Tamura

2018

J. Jpn. Petrol. Inst.

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Sulfation Patterns and the Amounts of Chondroitin Sulfate in the Diamond Squid,Thysanoteuthis rhombus

Cited by 19 publications

References 20 publications

Diamond Squid (Thysanoteuthis rhombus)-Derived Chondroitin Sulfate Stimulates Bone Healing within a Rat Calvarial Defect

Diamond Squid (Thysanoteuthis rhombus)-Derived Chondroitin Sulfate Stimulates Bone Healing within a Rat Calvarial Defect

Characterization of Glycosaminoglycans in Gaping and Intact Connective Tissues of Farmed Atlantic Salmon (Salmo salar) Fillets by Mass Spectrometry

Facile Synthesis of Biofunctional Oligosaccharides of Chondroitin Sulfate

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