Sulfolipid density dictates the extent of carbon nanodot interaction with chloroplast membranes

Kim, Kyoungtea; Jeon, Su‐Ji; Hu, Peiguang; Anastasia, Caroline; Beimers, William; Giraldo, Juan Pablo; Pedersen, Joel A.

doi:10.1039/d2en00158f

Cited by 7 publications

(13 citation statements)

References 73 publications

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“…This makes sense as the surface charge of LCO and cell membranes are electrostatically incompatible. For example, K. Kim et al (2022) used quartz crystal microbalance with dissipation monitoring to show that negatively charged nanodots do not interact/adhere to negatively charged plant biosurfaces due to electrostatic incompatibilities. But in terms of nutrient depletion, LCO has been reported to adsorb nutrients like phosphate (Laudadio et al, 2019), which could, in theory, partially explain some of the disturbances.…”

Section: Discussionmentioning

confidence: 99%

Physiological Impacts on Raphidocelis subcapitata in Response to Lithiated Cobalt Oxide Nanomaterials

Ostovich

Henke

Green

et al. 2023

Enviro Toxic and Chemistry

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Complex metal oxide nanomaterials, like lithiated cobalt oxide (LCO) nanosheets, are among the most widespread classes of nanomaterials on the market. Their ubiquitous application in battery storage technology drives their production to rates of environmental significance without sufficient infrastructure for proper disposal/recycling, thus posing a risk to ecosystem health and sustainability. The present study assesses the general toxicological impacts of LCO when exposed to Raphidocelis subcapitata; physiological endpoints relating to growth and energy production are considered. Algal growth inhibition was significantly increased at concentrations as low as 0.1 µg ml −1 , while exhibiting a median effect concentration of 0.057 µg ml −1 . The average biovolume of cells was significantly enlarged at 0.01 µg ml −1 , thus indicating increased instances of cell cycle arrest in LCO-treated cells. In addition, LCO-treated cells produced significantly less carbon biomass while significantly overproducing neutral lipid content starting at 0.1 µg ml −1 , thus indicating interference with CO 2 assimilation chemistry and/or carbon partitioning. However, the relative abundance of chlorophyll was significantly increased, likely to maximize light harvesting and compensate for photosynthetic interference. Cells that were treated with dissolved Li + /Co 2+ ions did not significantly impact any of the endpoints tested, suggesting that LCO phytotoxicity is mainly induced through nano-specific mechanisms rather than ion-specific ones. These results indicate that this type of nanomaterial can significantly impact the way this alga proliferates, as well as the way it produces and stores its energy, even at lower, sublethal, concentrations. Furthermore, impairments to crucial cellular pathways, like carbon assimilation, could potentially cause implications at the ecosystem level. Thus, in future work, it will be important to characterize the molecular mechanisms of LCO at the nano-bio interface.

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Section: Discussionmentioning

confidence: 99%

Physiological Impacts on Raphidocelis subcapitata in Response to Lithiated Cobalt Oxide Nanomaterials

Ostovich

Henke

Green

et al. 2023

Enviro Toxic and Chemistry

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“…The acoustic mass density analysis supports the role of electrostatics in determining the interactions between positively charged PEI-CD and negatively charged pectin. We recently reported that CD interactions with lipid membranes from chloroplasts are also dictated by electrostatic interactions . Similar to native and model plant cell walls, QCM-D analysis demonstrated that only the positively charged CD led to detectable attachment to the chloroplast model and native membranes compared to their negative and neutral counterparts.…”

Section: Resultsmentioning

confidence: 99%

“…We recently reported that CD interactions with lipid membranes from chloroplasts are also dictated by electrostatic interactions. 41 Similar to native and model plant cell walls, QCM-D analysis demonstrated that only the positively charged CD led to detectable attachment to the chloroplast model and native membranes compared to their negative and neutral counterparts. Overall, the high adsorption affinity and acoustic mass density on the pectin layer upon exposure to PEI-CD support the idea that electrostatics plays a key role in the interactions between the positively charged nanoparticles and pectin model cell wall surfaces.…”

Section: Real-time Interactions Of Carbon Dots With Pectin Model Surf...mentioning

confidence: 99%

“…XPS analysis of the N1s electrons in amide and amine groups (399.6 eV) in the presence of Ca 2+ indicated that the formation of quaternary ammonium cations between the amine groups of PEI-CDs and the carboxylic acids of pectin was not affected (Figure S11d). Interestingly, our previous study reports that CD attachment to model chloroplast membranes exhibited a different response to changes in ionic strength (KCl) 41 in a manner that is consistent with the electrical double layer compression of CDs on plant lipid membranes. Overall, our results indicate that Ca 2+ affects the initial absorption efficiency of the positively charged PEI-CD to the pectin surface but not their final accumulation on the model cell wall surface.…”

Section: Real-time Interactions Of Carbon Dots With Pectin Model Surf...mentioning

confidence: 99%

“…In these studies, the plant cell wall was identified as a main barrier for nanoparticle uptake into plant cells, but the mechanisms of these interactions were not elucidated. Studies in bacteria and green algae have reported opposite interactions and effects of nanoparticle charge on their cell walls while the underlying physical and chemical mechanisms of these interactions remain unknown. , Quantitative analysis of the interactions between nanoparticles and biosurfaces has largely focused on attachment to the organism lipid membranes − without addressing the outermost cell wall biosurface that is characteristic of major organism taxa including plants and algae. The mechanisms of interactions with bacterial cell walls formed by lipopolysaccharides and peptidoglycans cannot be extrapolated to those of cell walls of plants and algae having a distinct composition of cellulose, pectin, and other nonlipid-based molecules.…”

Section: Introductionmentioning

confidence: 99%

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Electrostatics Control Nanoparticle Interactions with Model and Native Cell Walls of Plants and Algae

Jeon,

Hu,

Kim

et al. 2023

Environ. Sci. Technol.

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A lack of mechanistic understanding of nanomaterial interactions with plants and algae cell walls limits the advancement of nanotechnology-based tools for sustainable agriculture. We systematically investigated the influence of nanoparticle charge on the interactions with model cell wall surfaces built with cellulose or pectin and performed a comparative analysis with native cell walls of Arabidopsis plants and green algae (Choleochaete). The high affinity of positively charged carbon dots (CDs) (46.0 ± 3.3 mV, 4.3 ± 1.5 nm) to both model and native cell walls was dominated by the strong ionic bonding between the surface amine groups of CDs and the carboxyl groups of pectin. In contrast, these CDs formed weaker hydrogen bonding with the hydroxyl groups of cellulose model surfaces. The CDs of similar size with negative (−46.2 ± 1.1 mV, 6.6 ± 3.8 nm) or neutral (−8.6 ± 1.3 mV, 4.3 ± 1.9 nm) ζ-potentials exhibited negligible interactions with cell walls. Real-time monitoring of CD interactions with model pectin cell walls indicated higher absorption efficiency (3.4 ± 1.3 10 −9 ) and acoustic mass density (313.3 ± 63.3 ng cm −2 ) for the positively charged CDs than negative and neutral counterparts (p < 0.001 and p < 0.01, respectively). The surface charge density of the positively charged CDs significantly enhanced these electrostatic interactions with cell walls, pointing to approaches to control nanoparticle binding to plant biosurfaces. Ca 2+ -induced cross-linking of pectin affected the initial absorption efficiency of the positively charged CD on cell wall surfaces (∼3.75 times lower) but not the accumulation of the nanoparticles on cell wall surfaces. This study developed model biosurfaces for elucidating fundamental interactions of nanomaterials with cell walls, a main barrier for nanomaterial translocation in plants and algae in the environment, and for the advancement of nanoenabled agriculture with a reduced environmental impact.

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Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Krasley,

Li,

Galeana

et al. 2024

Chem. Rev.

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Fluorescent carbon nanomaterials have broadly useful chemical and photophysical attributes that are conducive to applications in biology. In this review, we focus on materials whose photophysics allow for the use of these materials in biomedical and environmental applications, with emphasis on imaging, biosensing, and cargo delivery. The review focuses primarily on graphitic carbon nanomaterials including graphene and its derivatives, carbon nanotubes, as well as carbon dots and carbon nanohoops. Recent advances in and future prospects of these fields are discussed at depth, and where appropriate, references to reviews pertaining to older literature are provided.

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Sulfolipid density dictates the extent of carbon nanodot interaction with chloroplast membranes

Abstract: Mechanisms of nanomaterial delivery to plant chloroplasts have been explored to improve plant stress tolerance, promote photosynthesis, facilitate genetic engineering, and manufacture self-repairing biomaterials, fuels, and biopharmaceuticals. However, the molecular...

Cited by 7 publications

References 73 publications

Physiological Impacts on Raphidocelis subcapitata in Response to Lithiated Cobalt Oxide Nanomaterials

Physiological Impacts on Raphidocelis subcapitata in Response to Lithiated Cobalt Oxide Nanomaterials

Electrostatics Control Nanoparticle Interactions with Model and Native Cell Walls of Plants and Algae

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

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