Sulfonated polyaniline‐graphene oxide hybrids: Synthesis and effect of monomer composition on the electrochemical signal for direct DNA detection

Abstract

A variety of sulfonated polyaniline-graphene oxide (SPAN-GNO) nanocomposites based on GNO, aniline (ANI) and m-aminobenzenesulfonic acid (ABSA) are prepared via changing the mole ratio of ANI to ABSA for the comparison of DNA sensing behavior. Self-signals of SPAN-GNO are employed for estimating the effect of preparation conditions [component, monomer composition (mole ratio of ANI to ABSA), and reaction time] on DNA immobilization and hybridization detection. Then, we find herein that the mole ratio of ANI to ABSA plays a lead role over other factors on hybridiza-tion efficiency. Meanwhile, the parallel experiments using methylene blue as the classic indicator verifies this conclusion. The results show that, by comparison with other mole ratio SPAN-GNO nanocomposites-modified electrodes, the mole ratio (2:3) exhibits the widest dynamic detection range from 1.0 3 10 214 to 1.0 3 10 26 M, as well as the lowest detection limit (3.06 3 10 215 M).

Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

“…Similar behavior exerts polypyrrole, though its response to biochemical reactions with DNA is milder than that of polyaniline [22]. Appropriate DNA sensors were reported for the determination of anthracyclines [23,24,25,26], salicylates [22], isoproterenol [27], and phenothiazine drugs [18,28]. However, electroconductivity of polyaniline is observed in strongly acidic conditions.…”