Sulfur reducing bacteria from coal pits waste heaps of Chervonograd mining region

Diakiv, S. V.; Hnatush, S. О.; Moroz, O. M.; Prypin, O. Ya.; Kulachkovskyi, O. R; Bodnaruk, V. Y.

doi:10.30970/sbi.1002.453

Cited by 3 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excessive amounts of copper come into the environment from pesticides, in particular fungicides, sewage of galvanic plants. In the flooded quarries of sulphur deposits, waste water from industrial enterprises, waste heaps of coal mines, landfills of household and industrial waste, the content of Cu can grow by a thousand times (Frank & Lushnikov, 2006;Diakiv et al, 2016). In the technogenic reservoir which was formed in the place of the Yavoriv sulphur deposit quarry in recent years the content of copper (II) ions at depths of 0-70 m does not exceed 0.017-0.021 mg/l (Moroz et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Sulfidogenic and metal reducing activities of Desulfuromonas genus bacteria under the influence of copper chloride

et al. 2018

Self Cite

View full text Add to dashboard Cite

The selection of strains isolated from technogenically altered ecotopes and resistant to contamination, capable of metabolizing a wide range of pollutants is a task highly relevant for creation of new methods for environmental purification. Sulphur-reducing bacteria of the Desulfuromonas genus carry out dissimilatory reduction not only of S0 but also oxidized forms of metals. Intensity of anaerobic respiration of microorganisms in polluted environments is determined by level of their adaptation to stress factors, in particular, copper (II) compounds. The aim of this work was to investigate the influence of copper (II) chloride on H2S production by Desulfuromonas sp. strains isolated by us from Yavorivske Lake, to determine the efficiency of Cu2+ precipitation by hydrogen sulfide, to analyse the possibility of usage by bacteria of CuCl2 as an electron acceptor of anaerobic respiration and to study the influence of Cu2+ on usage by these microorganisms of ferric (III) citrate, potassium dichromate or manganese (IV) oxide as electron acceptors. Bacteria were grown under anaerobic conditions in Kravtsov-Sorokin medium. To study the influence of Cu2+ on production by bacteria of H2S, their cells were incubated with CuCl2 (0.5–4.0 mM), washed and cultivated in a medium with S0. To determine the level of Cu2+ binding by H2S, produced by bacteria, cells were grown in a medium with CuCl2 (0.5–4.0 mM) and S0. To investigate the ability of bacteria to use copper (II) ions as electron acceptors, they were cultivated in a medium with CuCl2 (1.74–10.41 mM). To study the influence of Cu2+ on usage by bacteria of metal compounds as electron acceptors, their cells were incubated with CuCl2 (0.5–4.0 mM), washed and cultivated in media with C6H5O7Fe, K2Cr2O7 or MnO2 (1.74–10.41 mM). Biomass was determined by the turbidimetric method. In the cultural liquid the content of H2S was determined quantitatively by the spectrophotometric method, qualitatively – presence of Cu2+. Content of CuS in the growth medium was determined by weight method. Desulfuromonas sp. bacteria was revealed to be resistant to 2.0–2.5 mM copper (II) ions. Under the influence of 3.0–4.0 mM CuCl2 in the incubation mixture, sulfidogenic activity of bacteria decreased more than twice. The efficiency of Cu2+ binding in form of CuS by H2S produced by bacteria reached 97.3–100.0% at presence in the medium with S0 of up to 1.5 mM CuCl2. Bacteria used CuCl2 (1.74–10.41 mM) as an electron acceptor in the process of anaerobic respiration. The addition of 2.5–3.0 mM CuCl2 to the incubation mixture caused inhibition of metal reducing activity of cells, growth of all strains in media with 1.74–10.41 mM ferric (III) citrate, potassium dichromate or manganese (IV) oxide as electron acceptors decreased by 2.6 times. Almost complete precipitation up to 1.5 mM copper (II) ions in form of CuS by H2S produced by bacteria and ability to reduce up to 10.41 mM CuCl2, C6H5O7Fe, K2Cr2O7 or MnO2 in the process of anaerobic respiration indicates a high adaptation of the bacteria strains investigated by us to stress factors, in particular, the influence of CuCl2. We have proved the possibility of using Desulfuromonas sp. in biotechnologies for purification of environments with complex contamination from copper (II) compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Sulfidogenic and metal reducing activities of Desulfuromonas genus bacteria under the influence of copper chloride

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Waste water treatment by exoelectrogenic bacteria isolated from technogenically transformed lands

Нnatush¹,

Maslovska²,

Sehin³

et al. 2019

View full text Add to dashboard Cite

The capacity of sulfur-reducing bacteria Desulfuromonas acetoxidans Geobacter sp. CB35 and Desulfuromusa sp. CB30 and green photosynthesizing bacteria Chlorobium limicola their growth in wastewater of industrial and municipal origin. The strains of exoelectrogens, which are characterized by resistance to heavy metal ions, were isolated from the man-made Yavorivske lake located in the Lviv Oblast in Ukraine (D. acetoxidans Ch. limicola Geobacter sp. CB 30 and Desulfuromusa sp. CB 35). Bacteria D. acetoxidans fuel cell (MFC) with the application of D. acetoxidans W/m 2 and the reduction of chemical oxygen demand of wastewater was 99%. The new approach to improving the MFC performance was investigated. It includes a combination of phototrophic microorganisms Ch. limicola and heterotrophic microorganisms, which reduce the content of nitrates, nitrites, ammonia, sulfates, sulfites, hydrogen sulfide, while simultaneously generating electric current.

show abstract

Physiological and Biochemical Characteristics of Desulfuromusa sp. CB30, Isolated from Coal Pits Waste Heaps

Diakiv¹,

Hnatush²,

Halushka³

2017

Mikrobiol. Z.

View full text Add to dashboard Cite

ФІЗІОЛОГО-БІОХІМІЧНІ ВЛАСТИВОСТІ DESULFUROMUSA SP. СВ30, ВИДІЛЕНИХ ІЗ ПОРОДНИХ ВІДВАЛІВ ВУГІЛЬНИХ ШАХТ Проведено дослідження фізіолого-біохімічних властивостей сірковідновлювальних бактерій Desulfuromusa sp. СВ30, виділених із породних відвалів вугільних шахт Червоноградського гірничопромислового району. Встановлено, що внесення фумарату до середовища Постгейта С для культивування досліджених бактерій спричиняє інтенсифікацію накопичення ними біомаси (у 1,56 рази) і гідроген сульфіду (у 1,23 рази) у порівнянні з контролем. Бактерії Desulfuromusa sp. СВ30 використовують фумарат одночасно як донор електронів, нагромаджуючи у культуральній рідині ацетат (до 3,47 г/л), та акцептор, відновлюючи його до сукцинату (4,05 г/л). За внесення натрію лактату і фумарату відбувається відновлення фумарату та його використання як додаткового джерела карбону, а натрій лактат використовується після вичерпання фумарату. Бактерії мають високу активність ензиму сульфурредуктази (55,6±9,67 мкМ H 2 S/ хв × мг білка, у діапазоні рН 7,5-9,0 і за температури 20-40ºС) та спорідненість сульфурредуктази до субстрату (Km=0,09±0,02 мкМ).

show abstract

Sulfur reducing bacteria from coal pits waste heaps of Chervonograd mining region

Cited by 3 publications

References 16 publications

Sulfidogenic and metal reducing activities of Desulfuromonas genus bacteria under the influence of copper chloride

Sulfidogenic and metal reducing activities of Desulfuromonas genus bacteria under the influence of copper chloride

Waste water treatment by exoelectrogenic bacteria isolated from technogenically transformed lands

Physiological and Biochemical Characteristics of Desulfuromusa sp. CB30, Isolated from Coal Pits Waste Heaps

Contact Info

Product

Resources

About